Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nuc...

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Detalles Bibliográficos
Autores principales: Carossino, Mariano, Ip, Hon S., Richt, Juergen A., Shultz, Kendra, Harper, Kimberly, Loynachan, Alan T., Del Piero, Fabio, Balasuriya, Udeni B. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406679/
https://www.ncbi.nlm.nih.gov/pubmed/32761270
http://dx.doi.org/10.1007/s00705-020-04737-w
Descripción
Sumario:In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

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