Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nuc...

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Autores principales: Carossino, Mariano, Ip, Hon S., Richt, Juergen A., Shultz, Kendra, Harper, Kimberly, Loynachan, Alan T., Del Piero, Fabio, Balasuriya, Udeni B. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406679/
https://www.ncbi.nlm.nih.gov/pubmed/32761270
http://dx.doi.org/10.1007/s00705-020-04737-w
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author Carossino, Mariano
Ip, Hon S.
Richt, Juergen A.
Shultz, Kendra
Harper, Kimberly
Loynachan, Alan T.
Del Piero, Fabio
Balasuriya, Udeni B. R.
author_facet Carossino, Mariano
Ip, Hon S.
Richt, Juergen A.
Shultz, Kendra
Harper, Kimberly
Loynachan, Alan T.
Del Piero, Fabio
Balasuriya, Udeni B. R.
author_sort Carossino, Mariano
collection PubMed
description In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
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spelling pubmed-74066792020-08-06 Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques Carossino, Mariano Ip, Hon S. Richt, Juergen A. Shultz, Kendra Harper, Kimberly Loynachan, Alan T. Del Piero, Fabio Balasuriya, Udeni B. R. Arch Virol Brief Report In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2. Springer Vienna 2020-08-05 2020 /pmc/articles/PMC7406679/ /pubmed/32761270 http://dx.doi.org/10.1007/s00705-020-04737-w Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Carossino, Mariano
Ip, Hon S.
Richt, Juergen A.
Shultz, Kendra
Harper, Kimberly
Loynachan, Alan T.
Del Piero, Fabio
Balasuriya, Udeni B. R.
Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques
title Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques
title_full Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques
title_fullStr Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques
title_full_unstemmed Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques
title_short Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques
title_sort detection of sars-cov-2 by rnascope(®)in situ hybridization and immunohistochemistry techniques
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406679/
https://www.ncbi.nlm.nih.gov/pubmed/32761270
http://dx.doi.org/10.1007/s00705-020-04737-w
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