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Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nuc...
Autores principales: | Carossino, Mariano, Ip, Hon S., Richt, Juergen A., Shultz, Kendra, Harper, Kimberly, Loynachan, Alan T., Del Piero, Fabio, Balasuriya, Udeni B. R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406679/ https://www.ncbi.nlm.nih.gov/pubmed/32761270 http://dx.doi.org/10.1007/s00705-020-04737-w |
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