Cargando…
Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
Sexuality is one of the fundamental mechanisms that work towards maintaining genetic diversity within a species. In diploid persimmons (Diospyros spp.), separated sexuality, the presence of separate male and female individuals (dioecy), is controlled by the Y chromosome-encoded small-RNA gene, OGI....
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406971/ https://www.ncbi.nlm.nih.gov/pubmed/32761076 http://dx.doi.org/10.1093/dnares/dsaa012 |
_version_ | 1783567525158584320 |
---|---|
author | Masuda, Kanae Yamamoto, Eiji Shirasawa, Kenta Onoue, Noriyuki Kono, Atsushi Ushijima, Koichiro Kubo, Yasutaka Tao, Ryutaro Henry, Isabelle M Akagi, Takashi |
author_facet | Masuda, Kanae Yamamoto, Eiji Shirasawa, Kenta Onoue, Noriyuki Kono, Atsushi Ushijima, Koichiro Kubo, Yasutaka Tao, Ryutaro Henry, Isabelle M Akagi, Takashi |
author_sort | Masuda, Kanae |
collection | PubMed |
description | Sexuality is one of the fundamental mechanisms that work towards maintaining genetic diversity within a species. In diploid persimmons (Diospyros spp.), separated sexuality, the presence of separate male and female individuals (dioecy), is controlled by the Y chromosome-encoded small-RNA gene, OGI. On the other hand, sexuality in hexaploid Oriental persimmon (Diospyros kaki) is more plastic, with OGI-bearing genetically male individuals, able to produce both male and female flowers (monoecy). This is thought to be linked to the partial inactivation of OGI by a retrotransposon insertion, resulting in DNA methylation of the OGI promoter region. To identify the genetic factors regulating branch sexual conversion, genome-wide correlation/association analyses were conducted using ddRAD-Seq data from an F(1) segregating population, and using both quantitative and diploidized genotypes, respectively. We found that allelic ratio at the Y-chromosomal region, including OGI, was correlated with male conversion based on quantitative genotypes, suggesting that OGI can be activated in cis in a dosage-dependent manner. Genome-wide association analysis based on diploidized genotypes, normalized for the effect of OGI allele dosage, detected three fundamental loci associated with male conversion. These loci underlie candidate genes, which could potentially act epigenetically for the activation of OGI expression. |
format | Online Article Text |
id | pubmed-7406971 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-74069712020-08-10 Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon Masuda, Kanae Yamamoto, Eiji Shirasawa, Kenta Onoue, Noriyuki Kono, Atsushi Ushijima, Koichiro Kubo, Yasutaka Tao, Ryutaro Henry, Isabelle M Akagi, Takashi DNA Res Research Article Sexuality is one of the fundamental mechanisms that work towards maintaining genetic diversity within a species. In diploid persimmons (Diospyros spp.), separated sexuality, the presence of separate male and female individuals (dioecy), is controlled by the Y chromosome-encoded small-RNA gene, OGI. On the other hand, sexuality in hexaploid Oriental persimmon (Diospyros kaki) is more plastic, with OGI-bearing genetically male individuals, able to produce both male and female flowers (monoecy). This is thought to be linked to the partial inactivation of OGI by a retrotransposon insertion, resulting in DNA methylation of the OGI promoter region. To identify the genetic factors regulating branch sexual conversion, genome-wide correlation/association analyses were conducted using ddRAD-Seq data from an F(1) segregating population, and using both quantitative and diploidized genotypes, respectively. We found that allelic ratio at the Y-chromosomal region, including OGI, was correlated with male conversion based on quantitative genotypes, suggesting that OGI can be activated in cis in a dosage-dependent manner. Genome-wide association analysis based on diploidized genotypes, normalized for the effect of OGI allele dosage, detected three fundamental loci associated with male conversion. These loci underlie candidate genes, which could potentially act epigenetically for the activation of OGI expression. Oxford University Press 2020-06-17 /pmc/articles/PMC7406971/ /pubmed/32761076 http://dx.doi.org/10.1093/dnares/dsaa012 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Masuda, Kanae Yamamoto, Eiji Shirasawa, Kenta Onoue, Noriyuki Kono, Atsushi Ushijima, Koichiro Kubo, Yasutaka Tao, Ryutaro Henry, Isabelle M Akagi, Takashi Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon |
title | Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon |
title_full | Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon |
title_fullStr | Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon |
title_full_unstemmed | Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon |
title_short | Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon |
title_sort | genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406971/ https://www.ncbi.nlm.nih.gov/pubmed/32761076 http://dx.doi.org/10.1093/dnares/dsaa012 |
work_keys_str_mv | AT masudakanae genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT yamamotoeiji genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT shirasawakenta genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT onouenoriyuki genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT konoatsushi genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT ushijimakoichiro genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT kuboyasutaka genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT taoryutaro genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT henryisabellem genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon AT akagitakashi genomewidestudyonthepolysomicgeneticfactorsconferringplasticityofflowersexualityinhexaploidpersimmon |