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Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon

Sexuality is one of the fundamental mechanisms that work towards maintaining genetic diversity within a species. In diploid persimmons (Diospyros spp.), separated sexuality, the presence of separate male and female individuals (dioecy), is controlled by the Y chromosome-encoded small-RNA gene, OGI....

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Autores principales: Masuda, Kanae, Yamamoto, Eiji, Shirasawa, Kenta, Onoue, Noriyuki, Kono, Atsushi, Ushijima, Koichiro, Kubo, Yasutaka, Tao, Ryutaro, Henry, Isabelle M, Akagi, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406971/
https://www.ncbi.nlm.nih.gov/pubmed/32761076
http://dx.doi.org/10.1093/dnares/dsaa012
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author Masuda, Kanae
Yamamoto, Eiji
Shirasawa, Kenta
Onoue, Noriyuki
Kono, Atsushi
Ushijima, Koichiro
Kubo, Yasutaka
Tao, Ryutaro
Henry, Isabelle M
Akagi, Takashi
author_facet Masuda, Kanae
Yamamoto, Eiji
Shirasawa, Kenta
Onoue, Noriyuki
Kono, Atsushi
Ushijima, Koichiro
Kubo, Yasutaka
Tao, Ryutaro
Henry, Isabelle M
Akagi, Takashi
author_sort Masuda, Kanae
collection PubMed
description Sexuality is one of the fundamental mechanisms that work towards maintaining genetic diversity within a species. In diploid persimmons (Diospyros spp.), separated sexuality, the presence of separate male and female individuals (dioecy), is controlled by the Y chromosome-encoded small-RNA gene, OGI. On the other hand, sexuality in hexaploid Oriental persimmon (Diospyros kaki) is more plastic, with OGI-bearing genetically male individuals, able to produce both male and female flowers (monoecy). This is thought to be linked to the partial inactivation of OGI by a retrotransposon insertion, resulting in DNA methylation of the OGI promoter region. To identify the genetic factors regulating branch sexual conversion, genome-wide correlation/association analyses were conducted using ddRAD-Seq data from an F(1) segregating population, and using both quantitative and diploidized genotypes, respectively. We found that allelic ratio at the Y-chromosomal region, including OGI, was correlated with male conversion based on quantitative genotypes, suggesting that OGI can be activated in cis in a dosage-dependent manner. Genome-wide association analysis based on diploidized genotypes, normalized for the effect of OGI allele dosage, detected three fundamental loci associated with male conversion. These loci underlie candidate genes, which could potentially act epigenetically for the activation of OGI expression.
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spelling pubmed-74069712020-08-10 Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon Masuda, Kanae Yamamoto, Eiji Shirasawa, Kenta Onoue, Noriyuki Kono, Atsushi Ushijima, Koichiro Kubo, Yasutaka Tao, Ryutaro Henry, Isabelle M Akagi, Takashi DNA Res Research Article Sexuality is one of the fundamental mechanisms that work towards maintaining genetic diversity within a species. In diploid persimmons (Diospyros spp.), separated sexuality, the presence of separate male and female individuals (dioecy), is controlled by the Y chromosome-encoded small-RNA gene, OGI. On the other hand, sexuality in hexaploid Oriental persimmon (Diospyros kaki) is more plastic, with OGI-bearing genetically male individuals, able to produce both male and female flowers (monoecy). This is thought to be linked to the partial inactivation of OGI by a retrotransposon insertion, resulting in DNA methylation of the OGI promoter region. To identify the genetic factors regulating branch sexual conversion, genome-wide correlation/association analyses were conducted using ddRAD-Seq data from an F(1) segregating population, and using both quantitative and diploidized genotypes, respectively. We found that allelic ratio at the Y-chromosomal region, including OGI, was correlated with male conversion based on quantitative genotypes, suggesting that OGI can be activated in cis in a dosage-dependent manner. Genome-wide association analysis based on diploidized genotypes, normalized for the effect of OGI allele dosage, detected three fundamental loci associated with male conversion. These loci underlie candidate genes, which could potentially act epigenetically for the activation of OGI expression. Oxford University Press 2020-06-17 /pmc/articles/PMC7406971/ /pubmed/32761076 http://dx.doi.org/10.1093/dnares/dsaa012 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Masuda, Kanae
Yamamoto, Eiji
Shirasawa, Kenta
Onoue, Noriyuki
Kono, Atsushi
Ushijima, Koichiro
Kubo, Yasutaka
Tao, Ryutaro
Henry, Isabelle M
Akagi, Takashi
Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
title Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
title_full Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
title_fullStr Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
title_full_unstemmed Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
title_short Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
title_sort genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406971/
https://www.ncbi.nlm.nih.gov/pubmed/32761076
http://dx.doi.org/10.1093/dnares/dsaa012
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