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Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value

Inflammatory breast cancer (IBC) is a rare yet aggressive breast cancer variant, associated with a poor prognosis. The major challenge for IBC is misdiagnosis due to the lack of molecular biomarkers. We profiled dysregulated expression of microRNAs (miRNAs) in primary samples of IBC and non-IBC tumo...

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Autores principales: Fahim, Sarah Atef, Abdullah, Mahmoud Salah, Espinoza-Sánchez, Nancy A., Hassan, Hebatallah, Ibrahim, Ayman M., Ahmed, Sarah Hamdy, Shakir, George, Badawy, Mohamed A., Zakhary, Nadia I., Greve, Burkhard, El-Shinawi, Mohamed, Götte, Martin, Ibrahim, Sherif Abdelaziz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407124/
https://www.ncbi.nlm.nih.gov/pubmed/32708601
http://dx.doi.org/10.3390/biom10071059
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author Fahim, Sarah Atef
Abdullah, Mahmoud Salah
Espinoza-Sánchez, Nancy A.
Hassan, Hebatallah
Ibrahim, Ayman M.
Ahmed, Sarah Hamdy
Shakir, George
Badawy, Mohamed A.
Zakhary, Nadia I.
Greve, Burkhard
El-Shinawi, Mohamed
Götte, Martin
Ibrahim, Sherif Abdelaziz
author_facet Fahim, Sarah Atef
Abdullah, Mahmoud Salah
Espinoza-Sánchez, Nancy A.
Hassan, Hebatallah
Ibrahim, Ayman M.
Ahmed, Sarah Hamdy
Shakir, George
Badawy, Mohamed A.
Zakhary, Nadia I.
Greve, Burkhard
El-Shinawi, Mohamed
Götte, Martin
Ibrahim, Sherif Abdelaziz
author_sort Fahim, Sarah Atef
collection PubMed
description Inflammatory breast cancer (IBC) is a rare yet aggressive breast cancer variant, associated with a poor prognosis. The major challenge for IBC is misdiagnosis due to the lack of molecular biomarkers. We profiled dysregulated expression of microRNAs (miRNAs) in primary samples of IBC and non-IBC tumors using human breast cancer miRNA PCR array. We discovered that 28 miRNAs were dysregulated (10 were upregulated, while 18 were underexpressed) in IBC vs. non-IBC tumors. We identified 128 hub genes, which are putative targets of the differentially expressed miRNAs and modulate important cancer biological processes. Furthermore, our qPCR analysis independently verified a significantly upregulated expression of miR-181b-5p, whereas a significant downregulation of miR-200b-3p, miR-200c-3p, and miR-203a-3p was detected in IBC tumors. Receiver operating characteristic (ROC) curves implied that the four miRNAs individually had a diagnostic accuracy in discriminating patients with IBC from non-IBC and that miR-203a-3p had the highest diagnostic value with an AUC of 0.821. Interestingly, a combination of miR-181b-5p, miR-200b-3p, and miR-200c-3p robustly improved the diagnostic accuracy, with an area under the curve (AUC) of 0.897. Intriguingly, qPCR revealed that the expression of zinc finger E box-binding homeobox 2 (ZEB2) mRNA, the putative target of miR-200b-3p, miR-200c-3p, and miR-203a-3p, was upregulated in IBC tumors. Overall, this study identified a set of miRNAs serving as potential biomarkers with diagnostic relevance for IBC.
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spelling pubmed-74071242020-08-11 Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value Fahim, Sarah Atef Abdullah, Mahmoud Salah Espinoza-Sánchez, Nancy A. Hassan, Hebatallah Ibrahim, Ayman M. Ahmed, Sarah Hamdy Shakir, George Badawy, Mohamed A. Zakhary, Nadia I. Greve, Burkhard El-Shinawi, Mohamed Götte, Martin Ibrahim, Sherif Abdelaziz Biomolecules Article Inflammatory breast cancer (IBC) is a rare yet aggressive breast cancer variant, associated with a poor prognosis. The major challenge for IBC is misdiagnosis due to the lack of molecular biomarkers. We profiled dysregulated expression of microRNAs (miRNAs) in primary samples of IBC and non-IBC tumors using human breast cancer miRNA PCR array. We discovered that 28 miRNAs were dysregulated (10 were upregulated, while 18 were underexpressed) in IBC vs. non-IBC tumors. We identified 128 hub genes, which are putative targets of the differentially expressed miRNAs and modulate important cancer biological processes. Furthermore, our qPCR analysis independently verified a significantly upregulated expression of miR-181b-5p, whereas a significant downregulation of miR-200b-3p, miR-200c-3p, and miR-203a-3p was detected in IBC tumors. Receiver operating characteristic (ROC) curves implied that the four miRNAs individually had a diagnostic accuracy in discriminating patients with IBC from non-IBC and that miR-203a-3p had the highest diagnostic value with an AUC of 0.821. Interestingly, a combination of miR-181b-5p, miR-200b-3p, and miR-200c-3p robustly improved the diagnostic accuracy, with an area under the curve (AUC) of 0.897. Intriguingly, qPCR revealed that the expression of zinc finger E box-binding homeobox 2 (ZEB2) mRNA, the putative target of miR-200b-3p, miR-200c-3p, and miR-203a-3p, was upregulated in IBC tumors. Overall, this study identified a set of miRNAs serving as potential biomarkers with diagnostic relevance for IBC. MDPI 2020-07-16 /pmc/articles/PMC7407124/ /pubmed/32708601 http://dx.doi.org/10.3390/biom10071059 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fahim, Sarah Atef
Abdullah, Mahmoud Salah
Espinoza-Sánchez, Nancy A.
Hassan, Hebatallah
Ibrahim, Ayman M.
Ahmed, Sarah Hamdy
Shakir, George
Badawy, Mohamed A.
Zakhary, Nadia I.
Greve, Burkhard
El-Shinawi, Mohamed
Götte, Martin
Ibrahim, Sherif Abdelaziz
Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value
title Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value
title_full Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value
title_fullStr Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value
title_full_unstemmed Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value
title_short Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value
title_sort inflammatory breast carcinoma: elevated microrna mir-181b-5p and reduced mir-200b-3p, mir-200c-3p, and mir-203a-3p expression as potential biomarkers with diagnostic value
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407124/
https://www.ncbi.nlm.nih.gov/pubmed/32708601
http://dx.doi.org/10.3390/biom10071059
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