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Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques
This work used in vivo MRI images of human colon wall motion to inform a biorelevant Dynamic Colon Model (DCM) to understand the interplay of wall motion, volume, viscosity, fluid, and particle motion within the colon lumen. Hydrodynamics and particle motion within the DCM were characterized using P...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407282/ https://www.ncbi.nlm.nih.gov/pubmed/32668624 http://dx.doi.org/10.3390/pharmaceutics12070659 |
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author | Stamatopoulos, Konstantinos Karandikar, Sharad Goldstein, Mark O’Farrell, Connor Marciani, Luca Sulaiman, Sarah Hoad, Caroline L. Simmons, Mark J. H. Batchelor, Hannah K. |
author_facet | Stamatopoulos, Konstantinos Karandikar, Sharad Goldstein, Mark O’Farrell, Connor Marciani, Luca Sulaiman, Sarah Hoad, Caroline L. Simmons, Mark J. H. Batchelor, Hannah K. |
author_sort | Stamatopoulos, Konstantinos |
collection | PubMed |
description | This work used in vivo MRI images of human colon wall motion to inform a biorelevant Dynamic Colon Model (DCM) to understand the interplay of wall motion, volume, viscosity, fluid, and particle motion within the colon lumen. Hydrodynamics and particle motion within the DCM were characterized using Positron Emission Tomography (PET) and Positron Emission Particle Tracking (PEPT), respectively. In vitro PET images showed that fluid of higher viscosity follows the wall motion with poor mixing, whereas good mixing was observed for a low viscosity fluid. PEPT data showed particle displacements comparable to the in vivo data. Increasing fluid viscosity favors the net forward propulsion of the tracked particles. The use of a floating particle demonstrated shorter residence times and greater velocities on the liquid surface, suggesting a surface wave that was moving faster than the bulk liquid. The DCM can provide an understanding of flow motion and behavior of particles with different buoyancy, which in turn may improve the design of drug formulations, whereby fragments of the dosage form and/or drug particles are suspended in the proximal colon. |
format | Online Article Text |
id | pubmed-7407282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74072822020-08-11 Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques Stamatopoulos, Konstantinos Karandikar, Sharad Goldstein, Mark O’Farrell, Connor Marciani, Luca Sulaiman, Sarah Hoad, Caroline L. Simmons, Mark J. H. Batchelor, Hannah K. Pharmaceutics Article This work used in vivo MRI images of human colon wall motion to inform a biorelevant Dynamic Colon Model (DCM) to understand the interplay of wall motion, volume, viscosity, fluid, and particle motion within the colon lumen. Hydrodynamics and particle motion within the DCM were characterized using Positron Emission Tomography (PET) and Positron Emission Particle Tracking (PEPT), respectively. In vitro PET images showed that fluid of higher viscosity follows the wall motion with poor mixing, whereas good mixing was observed for a low viscosity fluid. PEPT data showed particle displacements comparable to the in vivo data. Increasing fluid viscosity favors the net forward propulsion of the tracked particles. The use of a floating particle demonstrated shorter residence times and greater velocities on the liquid surface, suggesting a surface wave that was moving faster than the bulk liquid. The DCM can provide an understanding of flow motion and behavior of particles with different buoyancy, which in turn may improve the design of drug formulations, whereby fragments of the dosage form and/or drug particles are suspended in the proximal colon. MDPI 2020-07-13 /pmc/articles/PMC7407282/ /pubmed/32668624 http://dx.doi.org/10.3390/pharmaceutics12070659 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stamatopoulos, Konstantinos Karandikar, Sharad Goldstein, Mark O’Farrell, Connor Marciani, Luca Sulaiman, Sarah Hoad, Caroline L. Simmons, Mark J. H. Batchelor, Hannah K. Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques |
title | Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques |
title_full | Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques |
title_fullStr | Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques |
title_full_unstemmed | Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques |
title_short | Dynamic Colon Model (DCM): A Cine-MRI Informed Biorelevant In Vitro Model of the Human Proximal Large Intestine Characterized by Positron Imaging Techniques |
title_sort | dynamic colon model (dcm): a cine-mri informed biorelevant in vitro model of the human proximal large intestine characterized by positron imaging techniques |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407282/ https://www.ncbi.nlm.nih.gov/pubmed/32668624 http://dx.doi.org/10.3390/pharmaceutics12070659 |
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