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Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

BACKGROUND: The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity. Virus infectivity is conventionally examined with methods such as the plaque assay, even t...

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Detalles Bibliográficos
Autores principales: Nakaya, Yuki, Fukuda, Takashi, Ashiba, Hiroki, Yasuura, Masato, Fujimaki, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407439/
https://www.ncbi.nlm.nih.gov/pubmed/32762666
http://dx.doi.org/10.1186/s12879-020-05317-8