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Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy

[Image: see text] Using dielectric spectroscopy experiments performed at multiple temperatures and frequency ranges, we demonstrate how the chemotherapy drug paclitaxel changes the dynamic properties of water in a breast cancer cell line (MCF-7). From the measured data, we present evidence that trea...

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Autores principales: Martins, Murillo L., Bordallo, Heloisa N., Arrese-Igor, Silvia, Alegría, Angel, Colmenero de Leon, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407532/
https://www.ncbi.nlm.nih.gov/pubmed/32775862
http://dx.doi.org/10.1021/acsomega.0c00897
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author Martins, Murillo L.
Bordallo, Heloisa N.
Arrese-Igor, Silvia
Alegría, Angel
Colmenero de Leon, Juan
author_facet Martins, Murillo L.
Bordallo, Heloisa N.
Arrese-Igor, Silvia
Alegría, Angel
Colmenero de Leon, Juan
author_sort Martins, Murillo L.
collection PubMed
description [Image: see text] Using dielectric spectroscopy experiments performed at multiple temperatures and frequency ranges, we demonstrate how the chemotherapy drug paclitaxel changes the dynamic properties of water in a breast cancer cell line (MCF-7). From the measured data, we present evidence that treatment with paclitaxel leads to a slight increase in activation energy in a relaxation related to bulk-like water. More importantly, we also observe that paclitaxel changes the constraining imposed by the biological interfaces on hydration water, whose single-particle dynamics becomes slower and with higher activation energy. These variations are only observable after freezing the dynamics from other cellular components, such as proteins and DNAs, regardless of the state of the cells, that is, treated or not treated or even if the cells are no longer viable. Therefore, changes in water dynamics could be detected prior to those related to the global dynamics within the cellular environment.
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spelling pubmed-74075322020-08-07 Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy Martins, Murillo L. Bordallo, Heloisa N. Arrese-Igor, Silvia Alegría, Angel Colmenero de Leon, Juan ACS Omega [Image: see text] Using dielectric spectroscopy experiments performed at multiple temperatures and frequency ranges, we demonstrate how the chemotherapy drug paclitaxel changes the dynamic properties of water in a breast cancer cell line (MCF-7). From the measured data, we present evidence that treatment with paclitaxel leads to a slight increase in activation energy in a relaxation related to bulk-like water. More importantly, we also observe that paclitaxel changes the constraining imposed by the biological interfaces on hydration water, whose single-particle dynamics becomes slower and with higher activation energy. These variations are only observable after freezing the dynamics from other cellular components, such as proteins and DNAs, regardless of the state of the cells, that is, treated or not treated or even if the cells are no longer viable. Therefore, changes in water dynamics could be detected prior to those related to the global dynamics within the cellular environment. American Chemical Society 2020-07-20 /pmc/articles/PMC7407532/ /pubmed/32775862 http://dx.doi.org/10.1021/acsomega.0c00897 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Martins, Murillo L.
Bordallo, Heloisa N.
Arrese-Igor, Silvia
Alegría, Angel
Colmenero de Leon, Juan
Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy
title Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy
title_full Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy
title_fullStr Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy
title_full_unstemmed Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy
title_short Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy
title_sort effect of paclitaxel in the water dynamics of mcf-7 breast cancer cells revealed by dielectric spectroscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407532/
https://www.ncbi.nlm.nih.gov/pubmed/32775862
http://dx.doi.org/10.1021/acsomega.0c00897
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