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Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy
[Image: see text] Using dielectric spectroscopy experiments performed at multiple temperatures and frequency ranges, we demonstrate how the chemotherapy drug paclitaxel changes the dynamic properties of water in a breast cancer cell line (MCF-7). From the measured data, we present evidence that trea...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407532/ https://www.ncbi.nlm.nih.gov/pubmed/32775862 http://dx.doi.org/10.1021/acsomega.0c00897 |
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author | Martins, Murillo L. Bordallo, Heloisa N. Arrese-Igor, Silvia Alegría, Angel Colmenero de Leon, Juan |
author_facet | Martins, Murillo L. Bordallo, Heloisa N. Arrese-Igor, Silvia Alegría, Angel Colmenero de Leon, Juan |
author_sort | Martins, Murillo L. |
collection | PubMed |
description | [Image: see text] Using dielectric spectroscopy experiments performed at multiple temperatures and frequency ranges, we demonstrate how the chemotherapy drug paclitaxel changes the dynamic properties of water in a breast cancer cell line (MCF-7). From the measured data, we present evidence that treatment with paclitaxel leads to a slight increase in activation energy in a relaxation related to bulk-like water. More importantly, we also observe that paclitaxel changes the constraining imposed by the biological interfaces on hydration water, whose single-particle dynamics becomes slower and with higher activation energy. These variations are only observable after freezing the dynamics from other cellular components, such as proteins and DNAs, regardless of the state of the cells, that is, treated or not treated or even if the cells are no longer viable. Therefore, changes in water dynamics could be detected prior to those related to the global dynamics within the cellular environment. |
format | Online Article Text |
id | pubmed-7407532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74075322020-08-07 Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy Martins, Murillo L. Bordallo, Heloisa N. Arrese-Igor, Silvia Alegría, Angel Colmenero de Leon, Juan ACS Omega [Image: see text] Using dielectric spectroscopy experiments performed at multiple temperatures and frequency ranges, we demonstrate how the chemotherapy drug paclitaxel changes the dynamic properties of water in a breast cancer cell line (MCF-7). From the measured data, we present evidence that treatment with paclitaxel leads to a slight increase in activation energy in a relaxation related to bulk-like water. More importantly, we also observe that paclitaxel changes the constraining imposed by the biological interfaces on hydration water, whose single-particle dynamics becomes slower and with higher activation energy. These variations are only observable after freezing the dynamics from other cellular components, such as proteins and DNAs, regardless of the state of the cells, that is, treated or not treated or even if the cells are no longer viable. Therefore, changes in water dynamics could be detected prior to those related to the global dynamics within the cellular environment. American Chemical Society 2020-07-20 /pmc/articles/PMC7407532/ /pubmed/32775862 http://dx.doi.org/10.1021/acsomega.0c00897 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Martins, Murillo L. Bordallo, Heloisa N. Arrese-Igor, Silvia Alegría, Angel Colmenero de Leon, Juan Effect of Paclitaxel in the Water Dynamics of MCF-7 Breast Cancer Cells Revealed by Dielectric Spectroscopy |
title | Effect of Paclitaxel in the Water Dynamics of MCF-7
Breast Cancer Cells Revealed by Dielectric Spectroscopy |
title_full | Effect of Paclitaxel in the Water Dynamics of MCF-7
Breast Cancer Cells Revealed by Dielectric Spectroscopy |
title_fullStr | Effect of Paclitaxel in the Water Dynamics of MCF-7
Breast Cancer Cells Revealed by Dielectric Spectroscopy |
title_full_unstemmed | Effect of Paclitaxel in the Water Dynamics of MCF-7
Breast Cancer Cells Revealed by Dielectric Spectroscopy |
title_short | Effect of Paclitaxel in the Water Dynamics of MCF-7
Breast Cancer Cells Revealed by Dielectric Spectroscopy |
title_sort | effect of paclitaxel in the water dynamics of mcf-7
breast cancer cells revealed by dielectric spectroscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407532/ https://www.ncbi.nlm.nih.gov/pubmed/32775862 http://dx.doi.org/10.1021/acsomega.0c00897 |
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