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Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening

Directed evolution has revolutionized protein engineering. Still, enzyme optimization by random library screening remains sluggish, in large part due to futile probing of mutations that are catalytically neutral and/or impair stability and folding. FuncLib is a novel approach which uses phylogenetic...

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Detalles Bibliográficos
Autores principales:	Risso, Valeria A., Romero-Rivera, Adrian, Gutierrez-Rus, Luis I., Ortega-Muñoz, Mariano, Santoyo-Gonzalez, Francisco, Gavira, Jose A., Sanchez-Ruiz, Jose M., Kamerlin, Shina C. L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal Society of Chemistry 2020
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407621/ https://www.ncbi.nlm.nih.gov/pubmed/32832059 http://dx.doi.org/10.1039/d0sc01935f

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