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POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research
Prion diseases are fatal, transmissible neurodegenerative disorders whose pathogenesis is driven by the misfolding, self-templating and cell-to-cell spread of the prion protein. Other neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis and Hunti...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407827/ https://www.ncbi.nlm.nih.gov/pubmed/32698402 http://dx.doi.org/10.3390/biom10071079 |
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author | Pineau, Hailey Sim, Valerie |
author_facet | Pineau, Hailey Sim, Valerie |
author_sort | Pineau, Hailey |
collection | PubMed |
description | Prion diseases are fatal, transmissible neurodegenerative disorders whose pathogenesis is driven by the misfolding, self-templating and cell-to-cell spread of the prion protein. Other neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis and Huntington’s disease, share some of these prion-like features, with different aggregation-prone proteins. Consequently, researchers have begun to apply prion-specific techniques, like the prion organotypic slice culture assay (POSCA), to these disorders. In this review we explore the ways in which the prion phenomenon has been used in organotypic cultures to study neurodegenerative diseases from the perspective of protein aggregation and spreading, strain propagation, the role of glia in pathogenesis, and efficacy of drug treatments. We also present an overview of the advantages and disadvantages of this culture system compared to in vivo and in vitro models and provide suggestions for new directions. |
format | Online Article Text |
id | pubmed-7407827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74078272020-08-12 POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research Pineau, Hailey Sim, Valerie Biomolecules Review Prion diseases are fatal, transmissible neurodegenerative disorders whose pathogenesis is driven by the misfolding, self-templating and cell-to-cell spread of the prion protein. Other neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis and Huntington’s disease, share some of these prion-like features, with different aggregation-prone proteins. Consequently, researchers have begun to apply prion-specific techniques, like the prion organotypic slice culture assay (POSCA), to these disorders. In this review we explore the ways in which the prion phenomenon has been used in organotypic cultures to study neurodegenerative diseases from the perspective of protein aggregation and spreading, strain propagation, the role of glia in pathogenesis, and efficacy of drug treatments. We also present an overview of the advantages and disadvantages of this culture system compared to in vivo and in vitro models and provide suggestions for new directions. MDPI 2020-07-20 /pmc/articles/PMC7407827/ /pubmed/32698402 http://dx.doi.org/10.3390/biom10071079 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pineau, Hailey Sim, Valerie POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research |
title | POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research |
title_full | POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research |
title_fullStr | POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research |
title_full_unstemmed | POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research |
title_short | POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research |
title_sort | poscabilities: the application of the prion organotypic slice culture assay to neurodegenerative disease research |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407827/ https://www.ncbi.nlm.nih.gov/pubmed/32698402 http://dx.doi.org/10.3390/biom10071079 |
work_keys_str_mv | AT pineauhailey poscabilitiestheapplicationoftheprionorganotypicslicecultureassaytoneurodegenerativediseaseresearch AT simvalerie poscabilitiestheapplicationoftheprionorganotypicslicecultureassaytoneurodegenerativediseaseresearch |