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Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation
Granulocyte-monocyte colony stimulating factor (GM-CSF) is used as an adjuvant in various clinical and preclinical studies with contradictory results. These were attributed to opposing effects of GM-CSF on the immune or myeloid systems of the treated patients or to lack of optimal dosing regimens. T...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407964/ https://www.ncbi.nlm.nih.gov/pubmed/32668704 http://dx.doi.org/10.3390/cells9071683 |
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author | Moshe, Adi Izraely, Sivan Sagi-Assif, Orit Malka, Sapir Ben-Menachem, Shlomit Meshel, Tsipi Pasmanik-Chor, Metsada Hoon, Dave S.B. Witz, Isaac P. |
author_facet | Moshe, Adi Izraely, Sivan Sagi-Assif, Orit Malka, Sapir Ben-Menachem, Shlomit Meshel, Tsipi Pasmanik-Chor, Metsada Hoon, Dave S.B. Witz, Isaac P. |
author_sort | Moshe, Adi |
collection | PubMed |
description | Granulocyte-monocyte colony stimulating factor (GM-CSF) is used as an adjuvant in various clinical and preclinical studies with contradictory results. These were attributed to opposing effects of GM-CSF on the immune or myeloid systems of the treated patients or to lack of optimal dosing regimens. The results of the present study point to inter-tumor heterogeneity as a possible mechanism accounting for the contrasting responses to GM-CSF incorporating therapies. Employing xenograft models of human melanomas in nude mice developed in our lab, we detected differential functional responses of melanomas from different patients to GM-CSF both in vitro as well as in vivo. Whereas cells of one melanoma acquired pro metastatic features following exposure to GM-CSF, cells from another melanoma either did not respond or became less malignant. We propose that inter-melanoma heterogeneity as manifested by differential responses of melanoma cells (and perhaps also of other tumor) to GM-CSF may be developed into a predictive marker providing a tool to segregate melanoma patients who will benefit from GM-CSF therapy from those who will not. |
format | Online Article Text |
id | pubmed-7407964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74079642020-08-12 Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation Moshe, Adi Izraely, Sivan Sagi-Assif, Orit Malka, Sapir Ben-Menachem, Shlomit Meshel, Tsipi Pasmanik-Chor, Metsada Hoon, Dave S.B. Witz, Isaac P. Cells Article Granulocyte-monocyte colony stimulating factor (GM-CSF) is used as an adjuvant in various clinical and preclinical studies with contradictory results. These were attributed to opposing effects of GM-CSF on the immune or myeloid systems of the treated patients or to lack of optimal dosing regimens. The results of the present study point to inter-tumor heterogeneity as a possible mechanism accounting for the contrasting responses to GM-CSF incorporating therapies. Employing xenograft models of human melanomas in nude mice developed in our lab, we detected differential functional responses of melanomas from different patients to GM-CSF both in vitro as well as in vivo. Whereas cells of one melanoma acquired pro metastatic features following exposure to GM-CSF, cells from another melanoma either did not respond or became less malignant. We propose that inter-melanoma heterogeneity as manifested by differential responses of melanoma cells (and perhaps also of other tumor) to GM-CSF may be developed into a predictive marker providing a tool to segregate melanoma patients who will benefit from GM-CSF therapy from those who will not. MDPI 2020-07-13 /pmc/articles/PMC7407964/ /pubmed/32668704 http://dx.doi.org/10.3390/cells9071683 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moshe, Adi Izraely, Sivan Sagi-Assif, Orit Malka, Sapir Ben-Menachem, Shlomit Meshel, Tsipi Pasmanik-Chor, Metsada Hoon, Dave S.B. Witz, Isaac P. Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation |
title | Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation |
title_full | Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation |
title_fullStr | Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation |
title_full_unstemmed | Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation |
title_short | Inter-Tumor Heterogeneity—Melanomas Respond Differently to GM-CSF-Mediated Activation |
title_sort | inter-tumor heterogeneity—melanomas respond differently to gm-csf-mediated activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407964/ https://www.ncbi.nlm.nih.gov/pubmed/32668704 http://dx.doi.org/10.3390/cells9071683 |
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