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Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen

Tamoxifen (TAM) is a hormonal drug and is mainly used as an anti-estrogen in breast cancer patients. TAM binds to estrogen receptors (ERs), resulting in inhibition of estrogen signaling pathways and thus, a downregulation of cell proliferation. Cancer cells with negative or low ER expression will no...

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Autores principales: Alomari, Munther, Balasamy, Rabindran Jermy, Almohazey, Dana, Ravinayagam, Vijaya, Al Hamad, Mohammad, Ababneh, Deena, Bahmdan, Hiba, Alomari, Abdul-Hakeem, Mokadem, Zakaria, Elaissari, Abdelhamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408027/
https://www.ncbi.nlm.nih.gov/pubmed/32650474
http://dx.doi.org/10.3390/polym12071516
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author Alomari, Munther
Balasamy, Rabindran Jermy
Almohazey, Dana
Ravinayagam, Vijaya
Al Hamad, Mohammad
Ababneh, Deena
Bahmdan, Hiba
Alomari, Abdul-Hakeem
Mokadem, Zakaria
Elaissari, Abdelhamid
author_facet Alomari, Munther
Balasamy, Rabindran Jermy
Almohazey, Dana
Ravinayagam, Vijaya
Al Hamad, Mohammad
Ababneh, Deena
Bahmdan, Hiba
Alomari, Abdul-Hakeem
Mokadem, Zakaria
Elaissari, Abdelhamid
author_sort Alomari, Munther
collection PubMed
description Tamoxifen (TAM) is a hormonal drug and is mainly used as an anti-estrogen in breast cancer patients. TAM binds to estrogen receptors (ERs), resulting in inhibition of estrogen signaling pathways and thus, a downregulation of cell proliferation. Cancer cells with negative or low ER expression will not uptake TAM and will show low response. Poly (methyl methacrylate) (PMMA) nanoparticles were prepared using surfactant-free emulsion polymerization, then were loaded with Nile red (NR), which resulted in PMMA-NR. To enhance TAM delivery to cervical cancer cells (HELA), which is considered ER-negative, we loaded TAM and polymethyl methacrylate nanoparticles-Nile-red into silica (PMMA-NR-Si-TAM). The uptake and intracellular distribution were visualized by confocal laser scanning microscopy, and the in vitro cytotoxic activity was evaluated by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay using HELA and non-tumorigenic cell line HFF-1. The sensitivity of HELA (LC50: 207.31 µg/mL) and HFF-1 (LC50: 234.08 µg/mL) to free TAM was very low. However, after the encapsulation of TAM with PMMA-NR, the sensitivity significantly increased HELA (LC50: 71.83 µg/mL) and HFF-1 (LC50: 37.36 µg/mL). This indicates that TAM can be used for the treatment of ER-negative cervical cancer once conjugated to PMMA-NR nanoparticles. In addition, the PMMA-NR formulation appears to be highly suitable for cancer imaging and drug delivery.
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spelling pubmed-74080272020-08-12 Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen Alomari, Munther Balasamy, Rabindran Jermy Almohazey, Dana Ravinayagam, Vijaya Al Hamad, Mohammad Ababneh, Deena Bahmdan, Hiba Alomari, Abdul-Hakeem Mokadem, Zakaria Elaissari, Abdelhamid Polymers (Basel) Article Tamoxifen (TAM) is a hormonal drug and is mainly used as an anti-estrogen in breast cancer patients. TAM binds to estrogen receptors (ERs), resulting in inhibition of estrogen signaling pathways and thus, a downregulation of cell proliferation. Cancer cells with negative or low ER expression will not uptake TAM and will show low response. Poly (methyl methacrylate) (PMMA) nanoparticles were prepared using surfactant-free emulsion polymerization, then were loaded with Nile red (NR), which resulted in PMMA-NR. To enhance TAM delivery to cervical cancer cells (HELA), which is considered ER-negative, we loaded TAM and polymethyl methacrylate nanoparticles-Nile-red into silica (PMMA-NR-Si-TAM). The uptake and intracellular distribution were visualized by confocal laser scanning microscopy, and the in vitro cytotoxic activity was evaluated by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay using HELA and non-tumorigenic cell line HFF-1. The sensitivity of HELA (LC50: 207.31 µg/mL) and HFF-1 (LC50: 234.08 µg/mL) to free TAM was very low. However, after the encapsulation of TAM with PMMA-NR, the sensitivity significantly increased HELA (LC50: 71.83 µg/mL) and HFF-1 (LC50: 37.36 µg/mL). This indicates that TAM can be used for the treatment of ER-negative cervical cancer once conjugated to PMMA-NR nanoparticles. In addition, the PMMA-NR formulation appears to be highly suitable for cancer imaging and drug delivery. MDPI 2020-07-08 /pmc/articles/PMC7408027/ /pubmed/32650474 http://dx.doi.org/10.3390/polym12071516 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alomari, Munther
Balasamy, Rabindran Jermy
Almohazey, Dana
Ravinayagam, Vijaya
Al Hamad, Mohammad
Ababneh, Deena
Bahmdan, Hiba
Alomari, Abdul-Hakeem
Mokadem, Zakaria
Elaissari, Abdelhamid
Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen
title Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen
title_full Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen
title_fullStr Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen
title_full_unstemmed Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen
title_short Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen
title_sort nile red-poly(methyl methacrylate)/silica nanocomposite particles increase the sensitivity of cervical cancer cells to tamoxifen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408027/
https://www.ncbi.nlm.nih.gov/pubmed/32650474
http://dx.doi.org/10.3390/polym12071516
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