Cargando…

Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy

Organic and biological compounds (especially those related to the pharmaceutical industry) have always been of great interest for researchers due to their importance for the development of new drugs to diagnose, cure, treat or prevent disease. As many new API (active pharmaceutical ingredients) and...

Descripción completa

Detalles Bibliográficos
Autores principales: Das, Partha Pratim, Guzzinati, Giulio, Coll, Catalina, Gomez Perez, Alejandro, Nicolopoulos, Stavros, Estrade, Sonia, Peiro, Francesca, Verbeeck, Johan, Zompra, Aikaterini A., Galanis, Athanassios S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408036/
https://www.ncbi.nlm.nih.gov/pubmed/32605004
http://dx.doi.org/10.3390/polym12071434
_version_ 1783567744574160896
author Das, Partha Pratim
Guzzinati, Giulio
Coll, Catalina
Gomez Perez, Alejandro
Nicolopoulos, Stavros
Estrade, Sonia
Peiro, Francesca
Verbeeck, Johan
Zompra, Aikaterini A.
Galanis, Athanassios S.
author_facet Das, Partha Pratim
Guzzinati, Giulio
Coll, Catalina
Gomez Perez, Alejandro
Nicolopoulos, Stavros
Estrade, Sonia
Peiro, Francesca
Verbeeck, Johan
Zompra, Aikaterini A.
Galanis, Athanassios S.
author_sort Das, Partha Pratim
collection PubMed
description Organic and biological compounds (especially those related to the pharmaceutical industry) have always been of great interest for researchers due to their importance for the development of new drugs to diagnose, cure, treat or prevent disease. As many new API (active pharmaceutical ingredients) and their polymorphs are in nanocrystalline or in amorphous form blended with amorphous polymeric matrix (known as amorphous solid dispersion—ASD), their structural identification and characterization at nm scale with conventional X-Ray/Raman/IR techniques becomes difficult. During any API synthesis/production or in the formulated drug product, impurities must be identified and characterized. Electron energy loss spectroscopy (EELS) at high energy resolution by transmission electron microscope (TEM) is expected to be a promising technique to screen and identify the different (organic) compounds used in a typical pharmaceutical or biological system and to detect any impurities present, if any, during the synthesis or formulation process. In this work, we propose the use of monochromated TEM-EELS, to analyze selected peptides and organic compounds and their polymorphs. In order to validate EELS for fingerprinting (in low loss/optical region) and by further correlation with advanced DFT, simulations were utilized.
format Online
Article
Text
id pubmed-7408036
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74080362020-08-12 Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy Das, Partha Pratim Guzzinati, Giulio Coll, Catalina Gomez Perez, Alejandro Nicolopoulos, Stavros Estrade, Sonia Peiro, Francesca Verbeeck, Johan Zompra, Aikaterini A. Galanis, Athanassios S. Polymers (Basel) Article Organic and biological compounds (especially those related to the pharmaceutical industry) have always been of great interest for researchers due to their importance for the development of new drugs to diagnose, cure, treat or prevent disease. As many new API (active pharmaceutical ingredients) and their polymorphs are in nanocrystalline or in amorphous form blended with amorphous polymeric matrix (known as amorphous solid dispersion—ASD), their structural identification and characterization at nm scale with conventional X-Ray/Raman/IR techniques becomes difficult. During any API synthesis/production or in the formulated drug product, impurities must be identified and characterized. Electron energy loss spectroscopy (EELS) at high energy resolution by transmission electron microscope (TEM) is expected to be a promising technique to screen and identify the different (organic) compounds used in a typical pharmaceutical or biological system and to detect any impurities present, if any, during the synthesis or formulation process. In this work, we propose the use of monochromated TEM-EELS, to analyze selected peptides and organic compounds and their polymorphs. In order to validate EELS for fingerprinting (in low loss/optical region) and by further correlation with advanced DFT, simulations were utilized. MDPI 2020-06-27 /pmc/articles/PMC7408036/ /pubmed/32605004 http://dx.doi.org/10.3390/polym12071434 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Das, Partha Pratim
Guzzinati, Giulio
Coll, Catalina
Gomez Perez, Alejandro
Nicolopoulos, Stavros
Estrade, Sonia
Peiro, Francesca
Verbeeck, Johan
Zompra, Aikaterini A.
Galanis, Athanassios S.
Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy
title Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy
title_full Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy
title_fullStr Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy
title_full_unstemmed Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy
title_short Reliable Characterization of Organic & Pharmaceutical Compounds with High Resolution Monochromated EEL Spectroscopy
title_sort reliable characterization of organic & pharmaceutical compounds with high resolution monochromated eel spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408036/
https://www.ncbi.nlm.nih.gov/pubmed/32605004
http://dx.doi.org/10.3390/polym12071434
work_keys_str_mv AT dasparthapratim reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT guzzinatigiulio reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT collcatalina reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT gomezperezalejandro reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT nicolopoulosstavros reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT estradesonia reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT peirofrancesca reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT verbeeckjohan reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT zompraaikaterinia reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy
AT galanisathanassioss reliablecharacterizationoforganicpharmaceuticalcompoundswithhighresolutionmonochromatedeelspectroscopy