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Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
Engineered blood vessels generally recapitulate vascular function in vitro and can be utilized in drug discovery as a novel microphysiological system. Recently, various methods to fabricate vascular models in hydrogels have been reported to study the blood vessel functions in vitro; however, in gene...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408076/ https://www.ncbi.nlm.nih.gov/pubmed/32668567 http://dx.doi.org/10.3390/mi11070679 |
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author | Watanabe, Uran Sugiura, Shinji Kakehata, Masayuki Yanagawa, Fumiki Takagi, Toshiyuki Sumaru, Kimio Satoh, Taku Tamura, Masato Hosokawa, Yoichiroh Torizuka, Kenji Kanamori, Toshiyuki |
author_facet | Watanabe, Uran Sugiura, Shinji Kakehata, Masayuki Yanagawa, Fumiki Takagi, Toshiyuki Sumaru, Kimio Satoh, Taku Tamura, Masato Hosokawa, Yoichiroh Torizuka, Kenji Kanamori, Toshiyuki |
author_sort | Watanabe, Uran |
collection | PubMed |
description | Engineered blood vessels generally recapitulate vascular function in vitro and can be utilized in drug discovery as a novel microphysiological system. Recently, various methods to fabricate vascular models in hydrogels have been reported to study the blood vessel functions in vitro; however, in general, it is difficult to fabricate hollow structures with a designed size and structure with a tens of micrometers scale for blood vessel tissue engineering. This study reports a method to fabricate the hollow structures in photodegradable hydrogels prepared in a microfluidic device. An infrared femtosecond pulsed laser, employed to induce photodegradation via multi-photon excitation, was scanned in the hydrogel in a program-controlled manner for fabricating the designed hollow structures. The photodegradable hydrogel was prepared by a crosslinking reaction between an azide-modified gelatin solution and a dibenzocyclooctyl-terminated photocleavable tetra-arm polyethylene glycol crosslinker solution. After assessing the composition of the photodegradable hydrogel in terms of swelling and cell adhesion, the hydrogel prepared in the microfluidic device was processed by laser scanning to fabricate linear and branched hollow structures present in it. We introduced a microsphere suspension into the fabricated structure in photodegradable hydrogels, and confirmed the fabrication of perfusable hollow structures of designed patterns via the multi-photon excitation process. |
format | Online Article Text |
id | pubmed-7408076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74080762020-08-25 Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering Watanabe, Uran Sugiura, Shinji Kakehata, Masayuki Yanagawa, Fumiki Takagi, Toshiyuki Sumaru, Kimio Satoh, Taku Tamura, Masato Hosokawa, Yoichiroh Torizuka, Kenji Kanamori, Toshiyuki Micromachines (Basel) Article Engineered blood vessels generally recapitulate vascular function in vitro and can be utilized in drug discovery as a novel microphysiological system. Recently, various methods to fabricate vascular models in hydrogels have been reported to study the blood vessel functions in vitro; however, in general, it is difficult to fabricate hollow structures with a designed size and structure with a tens of micrometers scale for blood vessel tissue engineering. This study reports a method to fabricate the hollow structures in photodegradable hydrogels prepared in a microfluidic device. An infrared femtosecond pulsed laser, employed to induce photodegradation via multi-photon excitation, was scanned in the hydrogel in a program-controlled manner for fabricating the designed hollow structures. The photodegradable hydrogel was prepared by a crosslinking reaction between an azide-modified gelatin solution and a dibenzocyclooctyl-terminated photocleavable tetra-arm polyethylene glycol crosslinker solution. After assessing the composition of the photodegradable hydrogel in terms of swelling and cell adhesion, the hydrogel prepared in the microfluidic device was processed by laser scanning to fabricate linear and branched hollow structures present in it. We introduced a microsphere suspension into the fabricated structure in photodegradable hydrogels, and confirmed the fabrication of perfusable hollow structures of designed patterns via the multi-photon excitation process. MDPI 2020-07-13 /pmc/articles/PMC7408076/ /pubmed/32668567 http://dx.doi.org/10.3390/mi11070679 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Watanabe, Uran Sugiura, Shinji Kakehata, Masayuki Yanagawa, Fumiki Takagi, Toshiyuki Sumaru, Kimio Satoh, Taku Tamura, Masato Hosokawa, Yoichiroh Torizuka, Kenji Kanamori, Toshiyuki Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering |
title | Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering |
title_full | Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering |
title_fullStr | Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering |
title_full_unstemmed | Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering |
title_short | Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering |
title_sort | fabrication of hollow structures in photodegradable hydrogels using a multi-photon excitation process for blood vessel tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408076/ https://www.ncbi.nlm.nih.gov/pubmed/32668567 http://dx.doi.org/10.3390/mi11070679 |
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