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Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide

The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susc...

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Autores principales: Mas-Capdevila, Anna, Iglesias-Carres, Lisard, Arola-Arnal, Anna, Aragonès, Gerard, Muguerza, Begoña, Bravo, Francisca Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408493/
https://www.ncbi.nlm.nih.gov/pubmed/32630658
http://dx.doi.org/10.3390/biom10070992
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author Mas-Capdevila, Anna
Iglesias-Carres, Lisard
Arola-Arnal, Anna
Aragonès, Gerard
Muguerza, Begoña
Bravo, Francisca Isabel
author_facet Mas-Capdevila, Anna
Iglesias-Carres, Lisard
Arola-Arnal, Anna
Aragonès, Gerard
Muguerza, Begoña
Bravo, Francisca Isabel
author_sort Mas-Capdevila, Anna
collection PubMed
description The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption.
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spelling pubmed-74084932020-08-13 Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide Mas-Capdevila, Anna Iglesias-Carres, Lisard Arola-Arnal, Anna Aragonès, Gerard Muguerza, Begoña Bravo, Francisca Isabel Biomolecules Article The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption. MDPI 2020-07-02 /pmc/articles/PMC7408493/ /pubmed/32630658 http://dx.doi.org/10.3390/biom10070992 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mas-Capdevila, Anna
Iglesias-Carres, Lisard
Arola-Arnal, Anna
Aragonès, Gerard
Muguerza, Begoña
Bravo, Francisca Isabel
Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide
title Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide
title_full Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide
title_fullStr Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide
title_full_unstemmed Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide
title_short Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide
title_sort implication of opioid receptors in the antihypertensive effect of a novel chicken foot-derived peptide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408493/
https://www.ncbi.nlm.nih.gov/pubmed/32630658
http://dx.doi.org/10.3390/biom10070992
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