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Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide
The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408493/ https://www.ncbi.nlm.nih.gov/pubmed/32630658 http://dx.doi.org/10.3390/biom10070992 |
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author | Mas-Capdevila, Anna Iglesias-Carres, Lisard Arola-Arnal, Anna Aragonès, Gerard Muguerza, Begoña Bravo, Francisca Isabel |
author_facet | Mas-Capdevila, Anna Iglesias-Carres, Lisard Arola-Arnal, Anna Aragonès, Gerard Muguerza, Begoña Bravo, Francisca Isabel |
author_sort | Mas-Capdevila, Anna |
collection | PubMed |
description | The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption. |
format | Online Article Text |
id | pubmed-7408493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74084932020-08-13 Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide Mas-Capdevila, Anna Iglesias-Carres, Lisard Arola-Arnal, Anna Aragonès, Gerard Muguerza, Begoña Bravo, Francisca Isabel Biomolecules Article The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption. MDPI 2020-07-02 /pmc/articles/PMC7408493/ /pubmed/32630658 http://dx.doi.org/10.3390/biom10070992 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mas-Capdevila, Anna Iglesias-Carres, Lisard Arola-Arnal, Anna Aragonès, Gerard Muguerza, Begoña Bravo, Francisca Isabel Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide |
title | Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide |
title_full | Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide |
title_fullStr | Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide |
title_full_unstemmed | Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide |
title_short | Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide |
title_sort | implication of opioid receptors in the antihypertensive effect of a novel chicken foot-derived peptide |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408493/ https://www.ncbi.nlm.nih.gov/pubmed/32630658 http://dx.doi.org/10.3390/biom10070992 |
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