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Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes

Type 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte–endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition...

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Autores principales: Iannantuoni, Francesca, M. de Marañon, Aranzazu, Abad-Jiménez, Zaida, Canet, Francisco, Díaz-Pozo, Pedro, López-Domènech, Sandra, Morillas, Carlos, Rocha, Milagros, Víctor, Víctor M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408780/
https://www.ncbi.nlm.nih.gov/pubmed/32650465
http://dx.doi.org/10.3390/jcm9072155
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author Iannantuoni, Francesca
M. de Marañon, Aranzazu
Abad-Jiménez, Zaida
Canet, Francisco
Díaz-Pozo, Pedro
López-Domènech, Sandra
Morillas, Carlos
Rocha, Milagros
Víctor, Víctor M.
author_facet Iannantuoni, Francesca
M. de Marañon, Aranzazu
Abad-Jiménez, Zaida
Canet, Francisco
Díaz-Pozo, Pedro
López-Domènech, Sandra
Morillas, Carlos
Rocha, Milagros
Víctor, Víctor M.
author_sort Iannantuoni, Francesca
collection PubMed
description Type 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte–endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition and age-matched controls. We assessed anthropometric and metabolic parameters, oxidative stress and mitochondrial function by evaluating reactive oxygen species (ROS) production, mitochondrial ROS production, mitochondrial membrane potential and superoxide dismutase (SOD) and catalase (CAT) expression in polymorphonuclear leukocytes from type 1 diabetic patients. In addition, we evaluated interactions between leukocytes and human umbilical vein endothelial cells (HUVEC), and serum expression of adhesion molecules (P-selectin, VCAM-1 and ICAM-1), proinflammatory cytokines (IL-6 and TNFα) and myeloperoxidase (MPO). HbA(1C) and glucose levels were higher in diabetic patients than in control subjects, as expected. Mitochondrial function was altered and leukocyte–endothelium interactions were enhanced in diabetic patients, which was evident in the increase in total and mitochondrial ROS production, higher mitochondrial membrane potential, enhanced leukocyte rolling and adhesion, and decreased rolling velocity. Furthermore, we observed an increase in levels of adhesion molecules P-selectin, VCAM-1, and ICAM-1 in these subjects. In addition, type 1 diabetic patients exhibited an increase in proinflammatory mediators TNFα and MPO, and a decreased expression of SOD. The enhancement of leukocyte–endothelium interactions and proinflammatory markers correlated with glucose and HbA(1C)levels. Mitochondrial alteration, oxidative stress, and enhanced leukocyte–endothelium interactions are features of type 1 diabetes and may be related to cardiovascular implications.
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spelling pubmed-74087802020-08-13 Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes Iannantuoni, Francesca M. de Marañon, Aranzazu Abad-Jiménez, Zaida Canet, Francisco Díaz-Pozo, Pedro López-Domènech, Sandra Morillas, Carlos Rocha, Milagros Víctor, Víctor M. J Clin Med Article Type 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte–endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition and age-matched controls. We assessed anthropometric and metabolic parameters, oxidative stress and mitochondrial function by evaluating reactive oxygen species (ROS) production, mitochondrial ROS production, mitochondrial membrane potential and superoxide dismutase (SOD) and catalase (CAT) expression in polymorphonuclear leukocytes from type 1 diabetic patients. In addition, we evaluated interactions between leukocytes and human umbilical vein endothelial cells (HUVEC), and serum expression of adhesion molecules (P-selectin, VCAM-1 and ICAM-1), proinflammatory cytokines (IL-6 and TNFα) and myeloperoxidase (MPO). HbA(1C) and glucose levels were higher in diabetic patients than in control subjects, as expected. Mitochondrial function was altered and leukocyte–endothelium interactions were enhanced in diabetic patients, which was evident in the increase in total and mitochondrial ROS production, higher mitochondrial membrane potential, enhanced leukocyte rolling and adhesion, and decreased rolling velocity. Furthermore, we observed an increase in levels of adhesion molecules P-selectin, VCAM-1, and ICAM-1 in these subjects. In addition, type 1 diabetic patients exhibited an increase in proinflammatory mediators TNFα and MPO, and a decreased expression of SOD. The enhancement of leukocyte–endothelium interactions and proinflammatory markers correlated with glucose and HbA(1C)levels. Mitochondrial alteration, oxidative stress, and enhanced leukocyte–endothelium interactions are features of type 1 diabetes and may be related to cardiovascular implications. MDPI 2020-07-08 /pmc/articles/PMC7408780/ /pubmed/32650465 http://dx.doi.org/10.3390/jcm9072155 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iannantuoni, Francesca
M. de Marañon, Aranzazu
Abad-Jiménez, Zaida
Canet, Francisco
Díaz-Pozo, Pedro
López-Domènech, Sandra
Morillas, Carlos
Rocha, Milagros
Víctor, Víctor M.
Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_full Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_fullStr Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_full_unstemmed Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_short Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_sort mitochondrial alterations and enhanced human leukocyte/endothelial cell interactions in type 1 diabetes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408780/
https://www.ncbi.nlm.nih.gov/pubmed/32650465
http://dx.doi.org/10.3390/jcm9072155
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