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HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration
Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-relat...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408794/ https://www.ncbi.nlm.nih.gov/pubmed/32668747 http://dx.doi.org/10.3390/jcm9072217 |
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author | Sugita, Sunao Mandai, Michiko Hirami, Yasuhiko Takagi, Seiji Maeda, Tadao Fujihara, Masashi Matsuzaki, Mitsuhiro Yamamoto, Midori Iseki, Kyoko Hayashi, Naoko Hono, Ayumi Fujino, Shoko Koide, Naoshi Sakai, Noriko Shibata, Yumiko Terada, Motoki Nishida, Mitsuhiro Dohi, Hiromi Nomura, Masaki Amano, Naoki Sakaguchi, Hirokazu Hara, Chikako Maruyama, Kazuichi Daimon, Takashi Igeta, Masataka Oda, Toshihiko Shirono, Utako Tozaki, Misato Totani, Kota Sugiyama, Satoshi Nishida, Kohji Kurimoto, Yasuo Takahashi, Masayo |
author_facet | Sugita, Sunao Mandai, Michiko Hirami, Yasuhiko Takagi, Seiji Maeda, Tadao Fujihara, Masashi Matsuzaki, Mitsuhiro Yamamoto, Midori Iseki, Kyoko Hayashi, Naoko Hono, Ayumi Fujino, Shoko Koide, Naoshi Sakai, Noriko Shibata, Yumiko Terada, Motoki Nishida, Mitsuhiro Dohi, Hiromi Nomura, Masaki Amano, Naoki Sakaguchi, Hirokazu Hara, Chikako Maruyama, Kazuichi Daimon, Takashi Igeta, Masataka Oda, Toshihiko Shirono, Utako Tozaki, Misato Totani, Kota Sugiyama, Satoshi Nishida, Kohji Kurimoto, Yasuo Takahashi, Masayo |
author_sort | Sugita, Sunao |
collection | PubMed |
description | Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-related macular degeneration. In addition, local steroids without immunosuppressive medications were administered. We monitored immune rejections by routine ocular examinations as well as by lymphocytes-graft cells immune reaction (LGIR) tests using graft RPE and the patient’s blood cells. In all five of the cases that underwent iPS-RPE transplantation, the presence of graft cells was indicated by clumps or an area of increased pigmentation at 6 months, which became stable with no further abnormal growth in the graft during the 1-year observation period. Adverse events observed included corneal erosion, epiretinal membrane, retinal edema due to epiretinal membrane, elevated intraocular pressure, endophthalmitis, and mild immune rejection in the eye. In the one case exhibiting positive LGIR tests along with a slight fluid recurrence, we administrated local steroid therapy that subsequently resolved the suspected immune attacks. Although the cell delivery strategy must be further optimized, the present results suggest that it is possible to achieve stable survival and safety of iPS-RPE cell transplantation for a year. |
format | Online Article Text |
id | pubmed-7408794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74087942020-08-13 HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration Sugita, Sunao Mandai, Michiko Hirami, Yasuhiko Takagi, Seiji Maeda, Tadao Fujihara, Masashi Matsuzaki, Mitsuhiro Yamamoto, Midori Iseki, Kyoko Hayashi, Naoko Hono, Ayumi Fujino, Shoko Koide, Naoshi Sakai, Noriko Shibata, Yumiko Terada, Motoki Nishida, Mitsuhiro Dohi, Hiromi Nomura, Masaki Amano, Naoki Sakaguchi, Hirokazu Hara, Chikako Maruyama, Kazuichi Daimon, Takashi Igeta, Masataka Oda, Toshihiko Shirono, Utako Tozaki, Misato Totani, Kota Sugiyama, Satoshi Nishida, Kohji Kurimoto, Yasuo Takahashi, Masayo J Clin Med Article Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-related macular degeneration. In addition, local steroids without immunosuppressive medications were administered. We monitored immune rejections by routine ocular examinations as well as by lymphocytes-graft cells immune reaction (LGIR) tests using graft RPE and the patient’s blood cells. In all five of the cases that underwent iPS-RPE transplantation, the presence of graft cells was indicated by clumps or an area of increased pigmentation at 6 months, which became stable with no further abnormal growth in the graft during the 1-year observation period. Adverse events observed included corneal erosion, epiretinal membrane, retinal edema due to epiretinal membrane, elevated intraocular pressure, endophthalmitis, and mild immune rejection in the eye. In the one case exhibiting positive LGIR tests along with a slight fluid recurrence, we administrated local steroid therapy that subsequently resolved the suspected immune attacks. Although the cell delivery strategy must be further optimized, the present results suggest that it is possible to achieve stable survival and safety of iPS-RPE cell transplantation for a year. MDPI 2020-07-13 /pmc/articles/PMC7408794/ /pubmed/32668747 http://dx.doi.org/10.3390/jcm9072217 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sugita, Sunao Mandai, Michiko Hirami, Yasuhiko Takagi, Seiji Maeda, Tadao Fujihara, Masashi Matsuzaki, Mitsuhiro Yamamoto, Midori Iseki, Kyoko Hayashi, Naoko Hono, Ayumi Fujino, Shoko Koide, Naoshi Sakai, Noriko Shibata, Yumiko Terada, Motoki Nishida, Mitsuhiro Dohi, Hiromi Nomura, Masaki Amano, Naoki Sakaguchi, Hirokazu Hara, Chikako Maruyama, Kazuichi Daimon, Takashi Igeta, Masataka Oda, Toshihiko Shirono, Utako Tozaki, Misato Totani, Kota Sugiyama, Satoshi Nishida, Kohji Kurimoto, Yasuo Takahashi, Masayo HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration |
title | HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration |
title_full | HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration |
title_fullStr | HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration |
title_full_unstemmed | HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration |
title_short | HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration |
title_sort | hla-matched allogeneic ips cells-derived rpe transplantation for macular degeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408794/ https://www.ncbi.nlm.nih.gov/pubmed/32668747 http://dx.doi.org/10.3390/jcm9072217 |
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