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HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration

Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-relat...

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Autores principales: Sugita, Sunao, Mandai, Michiko, Hirami, Yasuhiko, Takagi, Seiji, Maeda, Tadao, Fujihara, Masashi, Matsuzaki, Mitsuhiro, Yamamoto, Midori, Iseki, Kyoko, Hayashi, Naoko, Hono, Ayumi, Fujino, Shoko, Koide, Naoshi, Sakai, Noriko, Shibata, Yumiko, Terada, Motoki, Nishida, Mitsuhiro, Dohi, Hiromi, Nomura, Masaki, Amano, Naoki, Sakaguchi, Hirokazu, Hara, Chikako, Maruyama, Kazuichi, Daimon, Takashi, Igeta, Masataka, Oda, Toshihiko, Shirono, Utako, Tozaki, Misato, Totani, Kota, Sugiyama, Satoshi, Nishida, Kohji, Kurimoto, Yasuo, Takahashi, Masayo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408794/
https://www.ncbi.nlm.nih.gov/pubmed/32668747
http://dx.doi.org/10.3390/jcm9072217
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author Sugita, Sunao
Mandai, Michiko
Hirami, Yasuhiko
Takagi, Seiji
Maeda, Tadao
Fujihara, Masashi
Matsuzaki, Mitsuhiro
Yamamoto, Midori
Iseki, Kyoko
Hayashi, Naoko
Hono, Ayumi
Fujino, Shoko
Koide, Naoshi
Sakai, Noriko
Shibata, Yumiko
Terada, Motoki
Nishida, Mitsuhiro
Dohi, Hiromi
Nomura, Masaki
Amano, Naoki
Sakaguchi, Hirokazu
Hara, Chikako
Maruyama, Kazuichi
Daimon, Takashi
Igeta, Masataka
Oda, Toshihiko
Shirono, Utako
Tozaki, Misato
Totani, Kota
Sugiyama, Satoshi
Nishida, Kohji
Kurimoto, Yasuo
Takahashi, Masayo
author_facet Sugita, Sunao
Mandai, Michiko
Hirami, Yasuhiko
Takagi, Seiji
Maeda, Tadao
Fujihara, Masashi
Matsuzaki, Mitsuhiro
Yamamoto, Midori
Iseki, Kyoko
Hayashi, Naoko
Hono, Ayumi
Fujino, Shoko
Koide, Naoshi
Sakai, Noriko
Shibata, Yumiko
Terada, Motoki
Nishida, Mitsuhiro
Dohi, Hiromi
Nomura, Masaki
Amano, Naoki
Sakaguchi, Hirokazu
Hara, Chikako
Maruyama, Kazuichi
Daimon, Takashi
Igeta, Masataka
Oda, Toshihiko
Shirono, Utako
Tozaki, Misato
Totani, Kota
Sugiyama, Satoshi
Nishida, Kohji
Kurimoto, Yasuo
Takahashi, Masayo
author_sort Sugita, Sunao
collection PubMed
description Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-related macular degeneration. In addition, local steroids without immunosuppressive medications were administered. We monitored immune rejections by routine ocular examinations as well as by lymphocytes-graft cells immune reaction (LGIR) tests using graft RPE and the patient’s blood cells. In all five of the cases that underwent iPS-RPE transplantation, the presence of graft cells was indicated by clumps or an area of increased pigmentation at 6 months, which became stable with no further abnormal growth in the graft during the 1-year observation period. Adverse events observed included corneal erosion, epiretinal membrane, retinal edema due to epiretinal membrane, elevated intraocular pressure, endophthalmitis, and mild immune rejection in the eye. In the one case exhibiting positive LGIR tests along with a slight fluid recurrence, we administrated local steroid therapy that subsequently resolved the suspected immune attacks. Although the cell delivery strategy must be further optimized, the present results suggest that it is possible to achieve stable survival and safety of iPS-RPE cell transplantation for a year.
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spelling pubmed-74087942020-08-13 HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration Sugita, Sunao Mandai, Michiko Hirami, Yasuhiko Takagi, Seiji Maeda, Tadao Fujihara, Masashi Matsuzaki, Mitsuhiro Yamamoto, Midori Iseki, Kyoko Hayashi, Naoko Hono, Ayumi Fujino, Shoko Koide, Naoshi Sakai, Noriko Shibata, Yumiko Terada, Motoki Nishida, Mitsuhiro Dohi, Hiromi Nomura, Masaki Amano, Naoki Sakaguchi, Hirokazu Hara, Chikako Maruyama, Kazuichi Daimon, Takashi Igeta, Masataka Oda, Toshihiko Shirono, Utako Tozaki, Misato Totani, Kota Sugiyama, Satoshi Nishida, Kohji Kurimoto, Yasuo Takahashi, Masayo J Clin Med Article Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-related macular degeneration. In addition, local steroids without immunosuppressive medications were administered. We monitored immune rejections by routine ocular examinations as well as by lymphocytes-graft cells immune reaction (LGIR) tests using graft RPE and the patient’s blood cells. In all five of the cases that underwent iPS-RPE transplantation, the presence of graft cells was indicated by clumps or an area of increased pigmentation at 6 months, which became stable with no further abnormal growth in the graft during the 1-year observation period. Adverse events observed included corneal erosion, epiretinal membrane, retinal edema due to epiretinal membrane, elevated intraocular pressure, endophthalmitis, and mild immune rejection in the eye. In the one case exhibiting positive LGIR tests along with a slight fluid recurrence, we administrated local steroid therapy that subsequently resolved the suspected immune attacks. Although the cell delivery strategy must be further optimized, the present results suggest that it is possible to achieve stable survival and safety of iPS-RPE cell transplantation for a year. MDPI 2020-07-13 /pmc/articles/PMC7408794/ /pubmed/32668747 http://dx.doi.org/10.3390/jcm9072217 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sugita, Sunao
Mandai, Michiko
Hirami, Yasuhiko
Takagi, Seiji
Maeda, Tadao
Fujihara, Masashi
Matsuzaki, Mitsuhiro
Yamamoto, Midori
Iseki, Kyoko
Hayashi, Naoko
Hono, Ayumi
Fujino, Shoko
Koide, Naoshi
Sakai, Noriko
Shibata, Yumiko
Terada, Motoki
Nishida, Mitsuhiro
Dohi, Hiromi
Nomura, Masaki
Amano, Naoki
Sakaguchi, Hirokazu
Hara, Chikako
Maruyama, Kazuichi
Daimon, Takashi
Igeta, Masataka
Oda, Toshihiko
Shirono, Utako
Tozaki, Misato
Totani, Kota
Sugiyama, Satoshi
Nishida, Kohji
Kurimoto, Yasuo
Takahashi, Masayo
HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration
title HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration
title_full HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration
title_fullStr HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration
title_full_unstemmed HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration
title_short HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration
title_sort hla-matched allogeneic ips cells-derived rpe transplantation for macular degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408794/
https://www.ncbi.nlm.nih.gov/pubmed/32668747
http://dx.doi.org/10.3390/jcm9072217
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