Cargando…

Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine th...

Descripción completa

Detalles Bibliográficos
Autores principales: Khan, Aman Ullah, Sayour, Ashraf E., Melzer, Falk, El-Soally, Sherif Abdel Ghafar Elsayed, Elschner, Mandy C., Shell, Waleed S., Moawad, Amira A., Mohamed, Shereen Aziz, Hendam, Ashraf, Roesler, Uwe, Neubauer, Heinrich, El-Adawy, Hosny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409340/
https://www.ncbi.nlm.nih.gov/pubmed/32668648
http://dx.doi.org/10.3390/microorganisms8071035
_version_ 1783568043884937216
author Khan, Aman Ullah
Sayour, Ashraf E.
Melzer, Falk
El-Soally, Sherif Abdel Ghafar Elsayed
Elschner, Mandy C.
Shell, Waleed S.
Moawad, Amira A.
Mohamed, Shereen Aziz
Hendam, Ashraf
Roesler, Uwe
Neubauer, Heinrich
El-Adawy, Hosny
author_facet Khan, Aman Ullah
Sayour, Ashraf E.
Melzer, Falk
El-Soally, Sherif Abdel Ghafar Elsayed
Elschner, Mandy C.
Shell, Waleed S.
Moawad, Amira A.
Mohamed, Shereen Aziz
Hendam, Ashraf
Roesler, Uwe
Neubauer, Heinrich
El-Adawy, Hosny
author_sort Khan, Aman Ullah
collection PubMed
description Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine the disease seroprevalence and identify the Brucella species in local camel holdings. In total, 381 serum samples were collected from male and female camels from Giza, Aswan, and Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples were serologically examined using the Rose–Bengal plate test (RBPT), indirect ELISA (i-ELISA), competitive ELISA (c-ELISA) and complement fixation test (CFT). Brucella antibodies were detected in 59 (15.5%), 87 (22.8%), 77 (20.2%) and 118 (31.0%) of sera by RBPT, i-ELISA, c-ELISA and CFT, respectively. Using real-time PCR, Brucella DNA was amplified in 32 (8.4%) seropositive samples including Brucella abortus (25/32), Brucella suis (5/32) and Brucella melitensis (2/32), defining a complex epidemiological status. To the best of our knowledge, this is the first study reporting Brucella suis DNA in camel serum. The risk-associated factors including age, sex, breed and geographical distribution were statistically analyzed, showing non-significant association with seroprevalence. The results of this study will raise awareness for camel brucellosis and help develop effective control strategies.
format Online
Article
Text
id pubmed-7409340
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74093402020-08-25 Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt Khan, Aman Ullah Sayour, Ashraf E. Melzer, Falk El-Soally, Sherif Abdel Ghafar Elsayed Elschner, Mandy C. Shell, Waleed S. Moawad, Amira A. Mohamed, Shereen Aziz Hendam, Ashraf Roesler, Uwe Neubauer, Heinrich El-Adawy, Hosny Microorganisms Article Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine the disease seroprevalence and identify the Brucella species in local camel holdings. In total, 381 serum samples were collected from male and female camels from Giza, Aswan, and Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples were serologically examined using the Rose–Bengal plate test (RBPT), indirect ELISA (i-ELISA), competitive ELISA (c-ELISA) and complement fixation test (CFT). Brucella antibodies were detected in 59 (15.5%), 87 (22.8%), 77 (20.2%) and 118 (31.0%) of sera by RBPT, i-ELISA, c-ELISA and CFT, respectively. Using real-time PCR, Brucella DNA was amplified in 32 (8.4%) seropositive samples including Brucella abortus (25/32), Brucella suis (5/32) and Brucella melitensis (2/32), defining a complex epidemiological status. To the best of our knowledge, this is the first study reporting Brucella suis DNA in camel serum. The risk-associated factors including age, sex, breed and geographical distribution were statistically analyzed, showing non-significant association with seroprevalence. The results of this study will raise awareness for camel brucellosis and help develop effective control strategies. MDPI 2020-07-13 /pmc/articles/PMC7409340/ /pubmed/32668648 http://dx.doi.org/10.3390/microorganisms8071035 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khan, Aman Ullah
Sayour, Ashraf E.
Melzer, Falk
El-Soally, Sherif Abdel Ghafar Elsayed
Elschner, Mandy C.
Shell, Waleed S.
Moawad, Amira A.
Mohamed, Shereen Aziz
Hendam, Ashraf
Roesler, Uwe
Neubauer, Heinrich
El-Adawy, Hosny
Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt
title Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt
title_full Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt
title_fullStr Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt
title_full_unstemmed Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt
title_short Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt
title_sort seroprevalence and molecular identification of brucella spp. in camels in egypt
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409340/
https://www.ncbi.nlm.nih.gov/pubmed/32668648
http://dx.doi.org/10.3390/microorganisms8071035
work_keys_str_mv AT khanamanullah seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT sayourashrafe seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT melzerfalk seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT elsoallysherifabdelghafarelsayed seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT elschnermandyc seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT shellwaleeds seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT moawadamiraa seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT mohamedshereenaziz seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT hendamashraf seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT roesleruwe seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT neubauerheinrich seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt
AT eladawyhosny seroprevalenceandmolecularidentificationofbrucellasppincamelsinegypt