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Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion
BACKGROUND: Numerous studies have shown that long noncoding RNAs play important roles in human cancer progression. Although zebrafish xenografts have recently become a novel in vivo model for human cancer research, whether such models can be used to study the function of long noncoding RNAs remains...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409423/ https://www.ncbi.nlm.nih.gov/pubmed/32774169 http://dx.doi.org/10.1186/s12935-020-01460-z |
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author | Shen, Wenyi Pu, Juan Sun, Jing Tan, Bing Wang, Wei Wang, Lili Cheng, Jianmeng Zuo, Yangsong |
author_facet | Shen, Wenyi Pu, Juan Sun, Jing Tan, Bing Wang, Wei Wang, Lili Cheng, Jianmeng Zuo, Yangsong |
author_sort | Shen, Wenyi |
collection | PubMed |
description | BACKGROUND: Numerous studies have shown that long noncoding RNAs play important roles in human cancer progression. Although zebrafish xenografts have recently become a novel in vivo model for human cancer research, whether such models can be used to study the function of long noncoding RNAs remains unknown. METHODS: In vitro studies validated the roles of LINC00152 in the proliferation and invasion of lung cancer cells. In vivo studies of zebrafish xenografts also confirmed these roles of LINC00152. In vivo confocal imaging was used to more accurately evaluate the function of LINC00152 in cell proliferation and migration. Pharmacological experiments were further performed to study the potential ability of LINC00152 downregulation combined with an EGFR inhibitor to treat tumors in cultured cells and the zebrafish xenograft model. RESULTS: Silencing of LINC00152 suppressed cell proliferation and invasion in SPCA1 and A549 lung cancer cell lines in vitro. In the zebrafish xenograft model, knockdown of LINC00152 reduced the proliferation and migration of lung cancer cells, as indicated by the two imaging methods at different magnifications. Moreover, the knockdown of LINC00152 enhanced the inhibition effect of afatinib for lung cancer progression in cultured cells and the zebrafish xenograft model. CONCLUSION: Our study reveals the oncogenic roles and potential for LINC00152 to be a target for tumor treatment in lung cancer using zebrafish xenograft models, and the findings suggest that this model could be used for functional and application studies of human long noncoding RNAs in tumor biology. |
format | Online Article Text |
id | pubmed-7409423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74094232020-08-07 Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion Shen, Wenyi Pu, Juan Sun, Jing Tan, Bing Wang, Wei Wang, Lili Cheng, Jianmeng Zuo, Yangsong Cancer Cell Int Primary Research BACKGROUND: Numerous studies have shown that long noncoding RNAs play important roles in human cancer progression. Although zebrafish xenografts have recently become a novel in vivo model for human cancer research, whether such models can be used to study the function of long noncoding RNAs remains unknown. METHODS: In vitro studies validated the roles of LINC00152 in the proliferation and invasion of lung cancer cells. In vivo studies of zebrafish xenografts also confirmed these roles of LINC00152. In vivo confocal imaging was used to more accurately evaluate the function of LINC00152 in cell proliferation and migration. Pharmacological experiments were further performed to study the potential ability of LINC00152 downregulation combined with an EGFR inhibitor to treat tumors in cultured cells and the zebrafish xenograft model. RESULTS: Silencing of LINC00152 suppressed cell proliferation and invasion in SPCA1 and A549 lung cancer cell lines in vitro. In the zebrafish xenograft model, knockdown of LINC00152 reduced the proliferation and migration of lung cancer cells, as indicated by the two imaging methods at different magnifications. Moreover, the knockdown of LINC00152 enhanced the inhibition effect of afatinib for lung cancer progression in cultured cells and the zebrafish xenograft model. CONCLUSION: Our study reveals the oncogenic roles and potential for LINC00152 to be a target for tumor treatment in lung cancer using zebrafish xenograft models, and the findings suggest that this model could be used for functional and application studies of human long noncoding RNAs in tumor biology. BioMed Central 2020-08-06 /pmc/articles/PMC7409423/ /pubmed/32774169 http://dx.doi.org/10.1186/s12935-020-01460-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Primary Research Shen, Wenyi Pu, Juan Sun, Jing Tan, Bing Wang, Wei Wang, Lili Cheng, Jianmeng Zuo, Yangsong Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion |
title | Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion |
title_full | Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion |
title_fullStr | Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion |
title_full_unstemmed | Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion |
title_short | Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion |
title_sort | zebrafish xenograft model of human lung cancer for studying the function of linc00152 in cell proliferation and invasion |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409423/ https://www.ncbi.nlm.nih.gov/pubmed/32774169 http://dx.doi.org/10.1186/s12935-020-01460-z |
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