Cargando…

Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)

BACKGROUND: Tillering is an important agronomic trait underlying the yields and reproduction of orchardgrass (Dactylis glomerata), an important perennial forage grass. Although some genes affecting tiller initiation have been identified, the tillering regulatory network is still largely unknown, esp...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Xiaoheng, Feng, Guangyan, Liang, Yueyang, Shuai, Yang, Liu, Qiuxu, Nie, Gang, Yang, Zhongfu, Hang, Linkai, Zhang, Xinquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409468/
https://www.ncbi.nlm.nih.gov/pubmed/32758131
http://dx.doi.org/10.1186/s12870-020-02582-2
_version_ 1783568068826365952
author Xu, Xiaoheng
Feng, Guangyan
Liang, Yueyang
Shuai, Yang
Liu, Qiuxu
Nie, Gang
Yang, Zhongfu
Hang, Linkai
Zhang, Xinquan
author_facet Xu, Xiaoheng
Feng, Guangyan
Liang, Yueyang
Shuai, Yang
Liu, Qiuxu
Nie, Gang
Yang, Zhongfu
Hang, Linkai
Zhang, Xinquan
author_sort Xu, Xiaoheng
collection PubMed
description BACKGROUND: Tillering is an important agronomic trait underlying the yields and reproduction of orchardgrass (Dactylis glomerata), an important perennial forage grass. Although some genes affecting tiller initiation have been identified, the tillering regulatory network is still largely unknown, especially in perennial forage grasses. Thus, unraveling the regulatory mechanisms of tillering in orchardgrass could be helpful in developing selective strategies for high-yield perennial grasses. In this study, we generated high-throughput RNA-sequencing data from multiple tissues of tillering stage plants to identify differentially expressed genes (DEGs) between high- and low-tillering orchardgrass genotypes. Gene Ontology and pathway enrichment analyses connecting the DEGs to tillering number diversity were conducted. RESULTS: In the present study, approximately 26,282 DEGs were identified between two orchardgrass genotypes, AKZ-NRGR667 (a high-tillering genotype) and D20170203 (a low-tillering genotype), which significantly differed in tiller number. Pathway enrichment analysis indicated that DEGs related to the biosynthesis of three classes of phytohormones, i.e., strigolactones (SLs), abscisic acid (ABA), and gibberellic acid (GA), as well as nitrogen metabolism dominated such differences between the high- and low-tillering genotypes. We also confirmed that under phosphorus deficiency, the expression level of the major SL biosynthesis genes encoding DWARF27 (D27), 9-cis-beta-carotene 9′,10′-cleaving dioxygenase (CCD7), carlactone synthase (CCD8), and more axillary branching1 (MAX1) proteins in the high-tillering orchardgrass genotype increased more slowly relative to the low-tillering genotype. CONCLUSIONS: Here, we used transcriptomic data to study the tillering mechanism of perennial forage grasses. We demonstrated that differential expression patterns of genes involved in SL, ABA, and GA biosynthesis may differentiate high- and low-tillering orchardgrass genotypes at the tillering stage. Furthermore, the core SL biosynthesis-associated genes in high-tillering orchardgrass were more insensitive than the low-tillering genotype to phosphorus deficiency which can lead to increases in SL biosynthesis, raising the possibility that there may be distinct SL biosynthesis way in tillering regulation in orchardgrass. Our research has revealed some candidate genes involved in the regulation of tillering in perennial grasses that is available for establishment of new breeding resources for high-yield perennial grasses and will serve as a new resource for future studies into molecular mechanism of tillering regulation in orchardgrass.
format Online
Article
Text
id pubmed-7409468
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-74094682020-08-07 Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.) Xu, Xiaoheng Feng, Guangyan Liang, Yueyang Shuai, Yang Liu, Qiuxu Nie, Gang Yang, Zhongfu Hang, Linkai Zhang, Xinquan BMC Plant Biol Research Article BACKGROUND: Tillering is an important agronomic trait underlying the yields and reproduction of orchardgrass (Dactylis glomerata), an important perennial forage grass. Although some genes affecting tiller initiation have been identified, the tillering regulatory network is still largely unknown, especially in perennial forage grasses. Thus, unraveling the regulatory mechanisms of tillering in orchardgrass could be helpful in developing selective strategies for high-yield perennial grasses. In this study, we generated high-throughput RNA-sequencing data from multiple tissues of tillering stage plants to identify differentially expressed genes (DEGs) between high- and low-tillering orchardgrass genotypes. Gene Ontology and pathway enrichment analyses connecting the DEGs to tillering number diversity were conducted. RESULTS: In the present study, approximately 26,282 DEGs were identified between two orchardgrass genotypes, AKZ-NRGR667 (a high-tillering genotype) and D20170203 (a low-tillering genotype), which significantly differed in tiller number. Pathway enrichment analysis indicated that DEGs related to the biosynthesis of three classes of phytohormones, i.e., strigolactones (SLs), abscisic acid (ABA), and gibberellic acid (GA), as well as nitrogen metabolism dominated such differences between the high- and low-tillering genotypes. We also confirmed that under phosphorus deficiency, the expression level of the major SL biosynthesis genes encoding DWARF27 (D27), 9-cis-beta-carotene 9′,10′-cleaving dioxygenase (CCD7), carlactone synthase (CCD8), and more axillary branching1 (MAX1) proteins in the high-tillering orchardgrass genotype increased more slowly relative to the low-tillering genotype. CONCLUSIONS: Here, we used transcriptomic data to study the tillering mechanism of perennial forage grasses. We demonstrated that differential expression patterns of genes involved in SL, ABA, and GA biosynthesis may differentiate high- and low-tillering orchardgrass genotypes at the tillering stage. Furthermore, the core SL biosynthesis-associated genes in high-tillering orchardgrass were more insensitive than the low-tillering genotype to phosphorus deficiency which can lead to increases in SL biosynthesis, raising the possibility that there may be distinct SL biosynthesis way in tillering regulation in orchardgrass. Our research has revealed some candidate genes involved in the regulation of tillering in perennial grasses that is available for establishment of new breeding resources for high-yield perennial grasses and will serve as a new resource for future studies into molecular mechanism of tillering regulation in orchardgrass. BioMed Central 2020-08-05 /pmc/articles/PMC7409468/ /pubmed/32758131 http://dx.doi.org/10.1186/s12870-020-02582-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Xu, Xiaoheng
Feng, Guangyan
Liang, Yueyang
Shuai, Yang
Liu, Qiuxu
Nie, Gang
Yang, Zhongfu
Hang, Linkai
Zhang, Xinquan
Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)
title Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)
title_full Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)
title_fullStr Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)
title_full_unstemmed Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)
title_short Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)
title_sort comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (dactylis glomerata l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409468/
https://www.ncbi.nlm.nih.gov/pubmed/32758131
http://dx.doi.org/10.1186/s12870-020-02582-2
work_keys_str_mv AT xuxiaoheng comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT fengguangyan comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT liangyueyang comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT shuaiyang comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT liuqiuxu comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT niegang comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT yangzhongfu comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT hanglinkai comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal
AT zhangxinquan comparativetranscriptomeanalysesrevealdifferentmechanismofhighandlowtilleringgenotypescontrollingtillergrowthinorchardgrassdactylisglomeratal