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Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation

Asian soybean rust caused by the fungal pathogen Phakopsora pachyrhizi is the most devastating disease of soybean. The host cultivar specificity of the pathogen shows considerable differentiation depending on the area and season of its emergence. Although resistance genes for P. pachyrhizi (Rpp) hav...

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Autores principales: Kashiwa, Takeshi, Muraki, Yukie, Yamanaka, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411041/
https://www.ncbi.nlm.nih.gov/pubmed/32764613
http://dx.doi.org/10.1038/s41598-020-70188-7
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author Kashiwa, Takeshi
Muraki, Yukie
Yamanaka, Naoki
author_facet Kashiwa, Takeshi
Muraki, Yukie
Yamanaka, Naoki
author_sort Kashiwa, Takeshi
collection PubMed
description Asian soybean rust caused by the fungal pathogen Phakopsora pachyrhizi is the most devastating disease of soybean. The host cultivar specificity of the pathogen shows considerable differentiation depending on the area and season of its emergence. Although resistance genes for P. pachyrhizi (Rpp) have been reported in several soybean varieties, the genetic background of these varieties is highly differentiated. Furthermore, some of the varieties harbor unknown genetic factors in addition to Rpp that could influence resistance reactions against the pathogen. In order to gain a comprehensive understanding of Rpp–P. pachyrhizi interactions, homogenous plant material harboring Rpp genes is necessary. In this study, we bred Rpp-near isogenic lines (Rpp-NILs), which retained identical plant characters originating from a single genetic background, and accordingly showed low-variant compatible/incompatible reactions against the pathogen. These Rpp-NILs can be used as genetic resources for studying P. pachyrhizi epidemiology and elucidating resistance mechanisms. Compatible/incompatible relationships between the soybean rust resistance gene Rpp and isolates of the pathogen P. pachyrhizi are clearly distinguishable using the Rpp-NILs bred in this study.
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spelling pubmed-74110412020-08-07 Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation Kashiwa, Takeshi Muraki, Yukie Yamanaka, Naoki Sci Rep Article Asian soybean rust caused by the fungal pathogen Phakopsora pachyrhizi is the most devastating disease of soybean. The host cultivar specificity of the pathogen shows considerable differentiation depending on the area and season of its emergence. Although resistance genes for P. pachyrhizi (Rpp) have been reported in several soybean varieties, the genetic background of these varieties is highly differentiated. Furthermore, some of the varieties harbor unknown genetic factors in addition to Rpp that could influence resistance reactions against the pathogen. In order to gain a comprehensive understanding of Rpp–P. pachyrhizi interactions, homogenous plant material harboring Rpp genes is necessary. In this study, we bred Rpp-near isogenic lines (Rpp-NILs), which retained identical plant characters originating from a single genetic background, and accordingly showed low-variant compatible/incompatible reactions against the pathogen. These Rpp-NILs can be used as genetic resources for studying P. pachyrhizi epidemiology and elucidating resistance mechanisms. Compatible/incompatible relationships between the soybean rust resistance gene Rpp and isolates of the pathogen P. pachyrhizi are clearly distinguishable using the Rpp-NILs bred in this study. Nature Publishing Group UK 2020-08-06 /pmc/articles/PMC7411041/ /pubmed/32764613 http://dx.doi.org/10.1038/s41598-020-70188-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kashiwa, Takeshi
Muraki, Yukie
Yamanaka, Naoki
Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation
title Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation
title_full Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation
title_fullStr Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation
title_full_unstemmed Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation
title_short Near-isogenic soybean lines carrying Asian soybean rust resistance genes for practical pathogenicity validation
title_sort near-isogenic soybean lines carrying asian soybean rust resistance genes for practical pathogenicity validation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411041/
https://www.ncbi.nlm.nih.gov/pubmed/32764613
http://dx.doi.org/10.1038/s41598-020-70188-7
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