Cargando…

Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice

Leukocyte cell-derived chemotaxin 2 (LECT2) is a hepatokine that causes skeletal muscle insulin resistance. The circulating levels of LECT2 are a possible biomarker that can predict weight cycling because they reflect liver fat and precede the onset of weight loss or gain. Herein, to clarify the dyn...

Descripción completa

Detalles Bibliográficos
Autores principales: Kikuchi, Akihiro, Takayama, Hiroaki, Tsugane, Hirohiko, Shiba, Kazuhiro, Chikamoto, Keita, Yamamoto, Tatsuya, Matsugo, Seiichi, Ishii, Kiyo-aki, Misu, Hirofumi, Takamura, Toshinari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411055/
https://www.ncbi.nlm.nih.gov/pubmed/32764719
http://dx.doi.org/10.1038/s41598-020-70192-x
_version_ 1783568296429223936
author Kikuchi, Akihiro
Takayama, Hiroaki
Tsugane, Hirohiko
Shiba, Kazuhiro
Chikamoto, Keita
Yamamoto, Tatsuya
Matsugo, Seiichi
Ishii, Kiyo-aki
Misu, Hirofumi
Takamura, Toshinari
author_facet Kikuchi, Akihiro
Takayama, Hiroaki
Tsugane, Hirohiko
Shiba, Kazuhiro
Chikamoto, Keita
Yamamoto, Tatsuya
Matsugo, Seiichi
Ishii, Kiyo-aki
Misu, Hirofumi
Takamura, Toshinari
author_sort Kikuchi, Akihiro
collection PubMed
description Leukocyte cell-derived chemotaxin 2 (LECT2) is a hepatokine that causes skeletal muscle insulin resistance. The circulating levels of LECT2 are a possible biomarker that can predict weight cycling because they reflect liver fat and precede the onset of weight loss or gain. Herein, to clarify the dynamics of this rapid change in serum LECT2 levels, we investigated the in vivo kinetics of LECT2, including its plasma half-life and tissue distribution, by injecting (125)I-labelled LECT2 into ICR mice and radioactivity tracing. The injected LECT2 was eliminated from the bloodstream within 10 min (approximate half-life, 5 min). In the kidneys, the radioactivity accumulated within 10 min after injection and declined thereafter. Conversely, the radioactivity in urine increased after 30 min of injection, indicating that LECT2 is mainly excreted by the kidneys into the urine. Finally, LECT2 accumulated in the skeletal muscle and liver until 30 min and 2 min after injection, respectively. LECT2 accumulation was not observed in the adipose tissue. These findings are in agreement with LECT2 action on the skeletal muscle. The present study indicates that LECT2 is a rapid-turnover protein, which renders the circulating level of LECT2 a useful rapid-response biomarker to predict body weight alterations.
format Online
Article
Text
id pubmed-7411055
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-74110552020-08-10 Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice Kikuchi, Akihiro Takayama, Hiroaki Tsugane, Hirohiko Shiba, Kazuhiro Chikamoto, Keita Yamamoto, Tatsuya Matsugo, Seiichi Ishii, Kiyo-aki Misu, Hirofumi Takamura, Toshinari Sci Rep Article Leukocyte cell-derived chemotaxin 2 (LECT2) is a hepatokine that causes skeletal muscle insulin resistance. The circulating levels of LECT2 are a possible biomarker that can predict weight cycling because they reflect liver fat and precede the onset of weight loss or gain. Herein, to clarify the dynamics of this rapid change in serum LECT2 levels, we investigated the in vivo kinetics of LECT2, including its plasma half-life and tissue distribution, by injecting (125)I-labelled LECT2 into ICR mice and radioactivity tracing. The injected LECT2 was eliminated from the bloodstream within 10 min (approximate half-life, 5 min). In the kidneys, the radioactivity accumulated within 10 min after injection and declined thereafter. Conversely, the radioactivity in urine increased after 30 min of injection, indicating that LECT2 is mainly excreted by the kidneys into the urine. Finally, LECT2 accumulated in the skeletal muscle and liver until 30 min and 2 min after injection, respectively. LECT2 accumulation was not observed in the adipose tissue. These findings are in agreement with LECT2 action on the skeletal muscle. The present study indicates that LECT2 is a rapid-turnover protein, which renders the circulating level of LECT2 a useful rapid-response biomarker to predict body weight alterations. Nature Publishing Group UK 2020-08-06 /pmc/articles/PMC7411055/ /pubmed/32764719 http://dx.doi.org/10.1038/s41598-020-70192-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kikuchi, Akihiro
Takayama, Hiroaki
Tsugane, Hirohiko
Shiba, Kazuhiro
Chikamoto, Keita
Yamamoto, Tatsuya
Matsugo, Seiichi
Ishii, Kiyo-aki
Misu, Hirofumi
Takamura, Toshinari
Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
title Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
title_full Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
title_fullStr Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
title_full_unstemmed Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
title_short Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
title_sort plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411055/
https://www.ncbi.nlm.nih.gov/pubmed/32764719
http://dx.doi.org/10.1038/s41598-020-70192-x
work_keys_str_mv AT kikuchiakihiro plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT takayamahiroaki plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT tsuganehirohiko plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT shibakazuhiro plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT chikamotokeita plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT yamamototatsuya plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT matsugoseiichi plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT ishiikiyoaki plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT misuhirofumi plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice
AT takamuratoshinari plasmahalflifeandtissuedistributionofleukocytecellderivedchemotaxin2inmice