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Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design

The enzymatic conversion of lignocellulosic material to sugars can provide a carbon source for the production of energy (fuels) and a wide range of renewable products. However, the efficiency of this conversion is impaired due to product (sugar) inhibition. Even though several studies investigate ho...

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Autores principales: Bussamra, Bianca Consorti, Meerman, Paulus, Viswanathan, Vidhvath, Mussatto, Solange I., Carvalho da Costa, Aline, van der Wielen, Luuk, Ottens, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411181/
https://www.ncbi.nlm.nih.gov/pubmed/32850627
http://dx.doi.org/10.3389/fchem.2020.00587
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author Bussamra, Bianca Consorti
Meerman, Paulus
Viswanathan, Vidhvath
Mussatto, Solange I.
Carvalho da Costa, Aline
van der Wielen, Luuk
Ottens, Marcel
author_facet Bussamra, Bianca Consorti
Meerman, Paulus
Viswanathan, Vidhvath
Mussatto, Solange I.
Carvalho da Costa, Aline
van der Wielen, Luuk
Ottens, Marcel
author_sort Bussamra, Bianca Consorti
collection PubMed
description The enzymatic conversion of lignocellulosic material to sugars can provide a carbon source for the production of energy (fuels) and a wide range of renewable products. However, the efficiency of this conversion is impaired due to product (sugar) inhibition. Even though several studies investigate how to overcome this challenge, concepts on the process to conduct the hydrolysis are still scarce in literature. Aqueous two-phase systems (ATPS) can be applied to design an extractive reaction due to their capacity to partition solutes to different phases in such a system. This work presents strategies on how to conduct extractive enzymatic hydrolysis in ATPS and how to explore the experimental results in order to design a feasible process. While only a limited number of ATPS was explored, the methods and strategies described could easily be applied to any further ATPS to be explored. We studied two promising ATPS as a subset of a previously high throughput screened large set of ATPS, providing two configurations of processes having the reaction in either the top phase or in the bottom phase. Enzymatic hydrolysis in these ATPS was performed to evaluate the partitioning of the substrate and the influence of solute partitioning on conversion. Because ATPS are able to partition inhibitors (sugar) between the phases, the conversion rate can be maintained. However, phase forming components should be selected to preserve the enzymatic activity. The experimental results presented here contribute to a feasible ATPS-based conceptual process design for the enzymatic conversion of lignocellulosic material.
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spelling pubmed-74111812020-08-25 Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design Bussamra, Bianca Consorti Meerman, Paulus Viswanathan, Vidhvath Mussatto, Solange I. Carvalho da Costa, Aline van der Wielen, Luuk Ottens, Marcel Front Chem Chemistry The enzymatic conversion of lignocellulosic material to sugars can provide a carbon source for the production of energy (fuels) and a wide range of renewable products. However, the efficiency of this conversion is impaired due to product (sugar) inhibition. Even though several studies investigate how to overcome this challenge, concepts on the process to conduct the hydrolysis are still scarce in literature. Aqueous two-phase systems (ATPS) can be applied to design an extractive reaction due to their capacity to partition solutes to different phases in such a system. This work presents strategies on how to conduct extractive enzymatic hydrolysis in ATPS and how to explore the experimental results in order to design a feasible process. While only a limited number of ATPS was explored, the methods and strategies described could easily be applied to any further ATPS to be explored. We studied two promising ATPS as a subset of a previously high throughput screened large set of ATPS, providing two configurations of processes having the reaction in either the top phase or in the bottom phase. Enzymatic hydrolysis in these ATPS was performed to evaluate the partitioning of the substrate and the influence of solute partitioning on conversion. Because ATPS are able to partition inhibitors (sugar) between the phases, the conversion rate can be maintained. However, phase forming components should be selected to preserve the enzymatic activity. The experimental results presented here contribute to a feasible ATPS-based conceptual process design for the enzymatic conversion of lignocellulosic material. Frontiers Media S.A. 2020-07-31 /pmc/articles/PMC7411181/ /pubmed/32850627 http://dx.doi.org/10.3389/fchem.2020.00587 Text en Copyright © 2020 Bussamra, Meerman, Viswanathan, Mussatto, Carvalho da Costa, van der Wielen and Ottens. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Bussamra, Bianca Consorti
Meerman, Paulus
Viswanathan, Vidhvath
Mussatto, Solange I.
Carvalho da Costa, Aline
van der Wielen, Luuk
Ottens, Marcel
Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design
title Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design
title_full Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design
title_fullStr Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design
title_full_unstemmed Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design
title_short Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design
title_sort enzymatic hydrolysis of sugarcane bagasse in aqueous two-phase systems (atps): exploration and conceptual process design
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411181/
https://www.ncbi.nlm.nih.gov/pubmed/32850627
http://dx.doi.org/10.3389/fchem.2020.00587
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