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An Optimized Method to Assess Viable Escherichia coli O157:H7 in Agricultural Soil Using Combined Propidium Monoazide Staining and Quantitative PCR

Agricultural soil contaminated by manure is becoming an important source for the transmission of foodborne pathogens. There is an urgent need for a rapid and accurate method for viable pathogen detection in agricultural soil samples. Propidium monoazide (PMA) is a DNA-binding dye that can inhibit th...

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Detalles Bibliográficos
Autores principales:	Fu, Yulong, Ye, Zhe, Jia, Yangyang, Fan, Jiahui, Hashmi, Muhammad Zaffar, Shen, Chaofeng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411311/ https://www.ncbi.nlm.nih.gov/pubmed/32849416 http://dx.doi.org/10.3389/fmicb.2020.01809

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