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Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells

Triple negative breast cancer (TNBC) is a breast cancer subtype associated with high rates of metastasis, heterogeneity, drug resistance and a poor prognosis. Extracellular vesicles (EVs) are vesicles of endosomal and plasma membrane origin, and are secreted by healthy and cancer cells. In cancer, E...

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Autores principales: Ramírez-Ricardo, Javier, Leal-Orta, Elizabeth, Martínez-Baeza, Elia, Ortiz-Mendoza, Carlos, Breton-Mora, Fernando, Herrera-Torres, Analy, Elizalde-Acosta, Irma, Cortes-Reynosa, Pedro, Thompson-Bonilla, Rocio, Salazar, Eduardo Perez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411406/
https://www.ncbi.nlm.nih.gov/pubmed/32582965
http://dx.doi.org/10.3892/mmr.2020.11259
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author Ramírez-Ricardo, Javier
Leal-Orta, Elizabeth
Martínez-Baeza, Elia
Ortiz-Mendoza, Carlos
Breton-Mora, Fernando
Herrera-Torres, Analy
Elizalde-Acosta, Irma
Cortes-Reynosa, Pedro
Thompson-Bonilla, Rocio
Salazar, Eduardo Perez
author_facet Ramírez-Ricardo, Javier
Leal-Orta, Elizabeth
Martínez-Baeza, Elia
Ortiz-Mendoza, Carlos
Breton-Mora, Fernando
Herrera-Torres, Analy
Elizalde-Acosta, Irma
Cortes-Reynosa, Pedro
Thompson-Bonilla, Rocio
Salazar, Eduardo Perez
author_sort Ramírez-Ricardo, Javier
collection PubMed
description Triple negative breast cancer (TNBC) is a breast cancer subtype associated with high rates of metastasis, heterogeneity, drug resistance and a poor prognosis. Extracellular vesicles (EVs) are vesicles of endosomal and plasma membrane origin, and are secreted by healthy and cancer cells. In cancer, EVs contribute to tumor progression by mediating escape from the immune system surveillance, and are involved in extracellular matrix degradation, invasion, angiogenesis, migration and metastasis. Furthermore, EVs have been identified in several human fluids. However, the role of EVs from patients with breast cancer in the migration and invasion of human breast cancer cells is not fully understood. The present study investigated whether EVs isolated from Mexican patients with breast cancer can induce cellular processes related to invasion in breast cancer. Moreover, plasma fractions enriched in EVs and deprived of platelet-derived EVs obtained from blood samples of 32 Mexican patients with biopsy-diagnosed breast cancer at different clinical stages who had not received treatment were analyzed. Furthermore, one control group was included, which consisted of 20 Mexican healthy females. The present results demonstrated that EVs from women with breast cancer promote migration and invasion, and increase matrix metalloproteinase (MMP)-2 and MMP-9 secretion in TNBC MDA-MB-231 cells. In addition, it was found that EVs from patients with breast cancer induced Src and focal adhesion kinase activation, and focal adhesions assembly with an increase in focal adhesions number, while the migration and invasion was dependent on Src activity. Collectively, EVs from Mexican patients with breast cancer induce migration and invasion via a Src-dependent pathway in TNBC MDA-MB-231 cells.
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spelling pubmed-74114062020-08-14 Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells Ramírez-Ricardo, Javier Leal-Orta, Elizabeth Martínez-Baeza, Elia Ortiz-Mendoza, Carlos Breton-Mora, Fernando Herrera-Torres, Analy Elizalde-Acosta, Irma Cortes-Reynosa, Pedro Thompson-Bonilla, Rocio Salazar, Eduardo Perez Mol Med Rep Articles Triple negative breast cancer (TNBC) is a breast cancer subtype associated with high rates of metastasis, heterogeneity, drug resistance and a poor prognosis. Extracellular vesicles (EVs) are vesicles of endosomal and plasma membrane origin, and are secreted by healthy and cancer cells. In cancer, EVs contribute to tumor progression by mediating escape from the immune system surveillance, and are involved in extracellular matrix degradation, invasion, angiogenesis, migration and metastasis. Furthermore, EVs have been identified in several human fluids. However, the role of EVs from patients with breast cancer in the migration and invasion of human breast cancer cells is not fully understood. The present study investigated whether EVs isolated from Mexican patients with breast cancer can induce cellular processes related to invasion in breast cancer. Moreover, plasma fractions enriched in EVs and deprived of platelet-derived EVs obtained from blood samples of 32 Mexican patients with biopsy-diagnosed breast cancer at different clinical stages who had not received treatment were analyzed. Furthermore, one control group was included, which consisted of 20 Mexican healthy females. The present results demonstrated that EVs from women with breast cancer promote migration and invasion, and increase matrix metalloproteinase (MMP)-2 and MMP-9 secretion in TNBC MDA-MB-231 cells. In addition, it was found that EVs from patients with breast cancer induced Src and focal adhesion kinase activation, and focal adhesions assembly with an increase in focal adhesions number, while the migration and invasion was dependent on Src activity. Collectively, EVs from Mexican patients with breast cancer induce migration and invasion via a Src-dependent pathway in TNBC MDA-MB-231 cells. D.A. Spandidos 2020-09 2020-06-18 /pmc/articles/PMC7411406/ /pubmed/32582965 http://dx.doi.org/10.3892/mmr.2020.11259 Text en Copyright: © Ramírez-Ricardo et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ramírez-Ricardo, Javier
Leal-Orta, Elizabeth
Martínez-Baeza, Elia
Ortiz-Mendoza, Carlos
Breton-Mora, Fernando
Herrera-Torres, Analy
Elizalde-Acosta, Irma
Cortes-Reynosa, Pedro
Thompson-Bonilla, Rocio
Salazar, Eduardo Perez
Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells
title Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells
title_full Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells
title_fullStr Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells
title_full_unstemmed Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells
title_short Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src-dependent pathway in MDA-MB-231 breast cancer cells
title_sort circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a src-dependent pathway in mda-mb-231 breast cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411406/
https://www.ncbi.nlm.nih.gov/pubmed/32582965
http://dx.doi.org/10.3892/mmr.2020.11259
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