Cargando…
Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease
BACKGROUND AND AIM: The T‐cell receptor (TCR) repertoire was assessed in response to various antigens and was considered to be associated with the pathogenesis of inflammatory bowel disease (IBD). Thus, we performed TCR repertoire analysis to examine the pathology of IBD from changes in the TCR repe...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wiley Publishing Asia Pty Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411559/ https://www.ncbi.nlm.nih.gov/pubmed/32782948 http://dx.doi.org/10.1002/jgh3.12305 |
_version_ | 1783568406546481152 |
---|---|
author | Kakuta, Yoichi Nakano, Takeru Naito, Takeo Watanabe, Kazuhiro Izumiyama, Yasuhiro Okamoto, Daisuke Ichikawa, Ryo Moroi, Rintaro Kuroha, Masatake Kanazawa, Yoshitake Kimura, Tomoya Shiga, Hisashi Naitoh, Takeshi Kinouchi, Yoshitaka Unno, Michiaki Masamune, Atsushi |
author_facet | Kakuta, Yoichi Nakano, Takeru Naito, Takeo Watanabe, Kazuhiro Izumiyama, Yasuhiro Okamoto, Daisuke Ichikawa, Ryo Moroi, Rintaro Kuroha, Masatake Kanazawa, Yoshitake Kimura, Tomoya Shiga, Hisashi Naitoh, Takeshi Kinouchi, Yoshitaka Unno, Michiaki Masamune, Atsushi |
author_sort | Kakuta, Yoichi |
collection | PubMed |
description | BACKGROUND AND AIM: The T‐cell receptor (TCR) repertoire was assessed in response to various antigens and was considered to be associated with the pathogenesis of inflammatory bowel disease (IBD). Thus, we performed TCR repertoire analysis to examine the pathology of IBD from changes in the TCR repertoire of memory T cells in the intestinal lamina propria mononuclear cells (LPMCs) and peripheral blood mononuclear cells (PBMCs) of patients with IBD. METHODS: LPMCs in the surgical specimens and PBMCs were isolated from 12 patients with IBD (5 patients with ulcerative colitis [UC] and 7 patients with Crohn's disease [CD]). PBMCs were collected from 10 healthy individuals as controls. Comprehensive TCR sequence analyses of adaptor‐ligation polymerase chain reaction (PCR) products were performed using MiSeq. RESULTS: The diversity of TCR‐α and TCR‐β in PBMCs was significantly lower in patients with IBD than that in controls (P = 0.00084 and 0.0013, respectively). Comparisons of TCR diversity in LPMCs and PBMCs between CD and UC showed that the diversity in LPMC was not affected by diseases, whereas that in PBMCs was significantly lower in CD than in UC (P = 0.045 and 0.049, respectively). Some TCR clones may have shown a specific increase or decrease in CD and UC, and many clones were common to both LPMCs and PBMCs in the same patients. CONCLUSION: The diversity of TCR clones in LPMCs and PBMCs in patients with IBD was significantly lower than that of PBMCs in controls. TCR diversity in PBMCs was particularly low in patients with CD. |
format | Online Article Text |
id | pubmed-7411559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wiley Publishing Asia Pty Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-74115592020-08-10 Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease Kakuta, Yoichi Nakano, Takeru Naito, Takeo Watanabe, Kazuhiro Izumiyama, Yasuhiro Okamoto, Daisuke Ichikawa, Ryo Moroi, Rintaro Kuroha, Masatake Kanazawa, Yoshitake Kimura, Tomoya Shiga, Hisashi Naitoh, Takeshi Kinouchi, Yoshitaka Unno, Michiaki Masamune, Atsushi JGH Open Original Articles BACKGROUND AND AIM: The T‐cell receptor (TCR) repertoire was assessed in response to various antigens and was considered to be associated with the pathogenesis of inflammatory bowel disease (IBD). Thus, we performed TCR repertoire analysis to examine the pathology of IBD from changes in the TCR repertoire of memory T cells in the intestinal lamina propria mononuclear cells (LPMCs) and peripheral blood mononuclear cells (PBMCs) of patients with IBD. METHODS: LPMCs in the surgical specimens and PBMCs were isolated from 12 patients with IBD (5 patients with ulcerative colitis [UC] and 7 patients with Crohn's disease [CD]). PBMCs were collected from 10 healthy individuals as controls. Comprehensive TCR sequence analyses of adaptor‐ligation polymerase chain reaction (PCR) products were performed using MiSeq. RESULTS: The diversity of TCR‐α and TCR‐β in PBMCs was significantly lower in patients with IBD than that in controls (P = 0.00084 and 0.0013, respectively). Comparisons of TCR diversity in LPMCs and PBMCs between CD and UC showed that the diversity in LPMC was not affected by diseases, whereas that in PBMCs was significantly lower in CD than in UC (P = 0.045 and 0.049, respectively). Some TCR clones may have shown a specific increase or decrease in CD and UC, and many clones were common to both LPMCs and PBMCs in the same patients. CONCLUSION: The diversity of TCR clones in LPMCs and PBMCs in patients with IBD was significantly lower than that of PBMCs in controls. TCR diversity in PBMCs was particularly low in patients with CD. Wiley Publishing Asia Pty Ltd 2020-02-06 /pmc/articles/PMC7411559/ /pubmed/32782948 http://dx.doi.org/10.1002/jgh3.12305 Text en © 2020 The Authors. JGH Open: An open access journal of gastroenterology and hepatology published by Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Kakuta, Yoichi Nakano, Takeru Naito, Takeo Watanabe, Kazuhiro Izumiyama, Yasuhiro Okamoto, Daisuke Ichikawa, Ryo Moroi, Rintaro Kuroha, Masatake Kanazawa, Yoshitake Kimura, Tomoya Shiga, Hisashi Naitoh, Takeshi Kinouchi, Yoshitaka Unno, Michiaki Masamune, Atsushi Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease |
title | Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease |
title_full | Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease |
title_fullStr | Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease |
title_full_unstemmed | Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease |
title_short | Repertoire analysis of memory T‐cell receptors in Japanese patients with inflammatory bowel disease |
title_sort | repertoire analysis of memory t‐cell receptors in japanese patients with inflammatory bowel disease |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411559/ https://www.ncbi.nlm.nih.gov/pubmed/32782948 http://dx.doi.org/10.1002/jgh3.12305 |
work_keys_str_mv | AT kakutayoichi repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT nakanotakeru repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT naitotakeo repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT watanabekazuhiro repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT izumiyamayasuhiro repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT okamotodaisuke repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT ichikawaryo repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT moroirintaro repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT kurohamasatake repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT kanazawayoshitake repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT kimuratomoya repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT shigahisashi repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT naitohtakeshi repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT kinouchiyoshitaka repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT unnomichiaki repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease AT masamuneatsushi repertoireanalysisofmemorytcellreceptorsinjapanesepatientswithinflammatoryboweldisease |