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Screening soybean cyst nematode effectors for their ability to suppress plant immunity
The soybean cyst nematode (SCN), Heterodera glycines, is one of the most destructive pathogens of soybeans. SCN is an obligate and sedentary parasite that transforms host plant root cells into an elaborate permanent feeding site, a syncytium. Formation and maintenance of a viable syncytium is an abs...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411561/ https://www.ncbi.nlm.nih.gov/pubmed/32672422 http://dx.doi.org/10.1111/mpp.12972 |
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author | Pogorelko, Gennady Wang, Jianying Juvale, Parijat S. Mitchum, Melissa G. Baum, Thomas J. |
author_facet | Pogorelko, Gennady Wang, Jianying Juvale, Parijat S. Mitchum, Melissa G. Baum, Thomas J. |
author_sort | Pogorelko, Gennady |
collection | PubMed |
description | The soybean cyst nematode (SCN), Heterodera glycines, is one of the most destructive pathogens of soybeans. SCN is an obligate and sedentary parasite that transforms host plant root cells into an elaborate permanent feeding site, a syncytium. Formation and maintenance of a viable syncytium is an absolute requirement for nematode growth and reproduction. In turn, sensing pathogen attack, plants activate defence responses and may trigger programmed cell death at the sites of infection. For successful parasitism, H. glycines must suppress these host defence responses to establish and maintain viable syncytia. Similar to other pathogens, H. glycines engages in these molecular interactions with its host via effector proteins. The goal of this study was to conduct a comprehensive screen to identify H. glycines effectors that interfere with plant immune responses. We used Nicotiana benthamiana plants infected by Pseudomonas syringae and Pseudomonas fluorescens strains. Using these pathosystems, we screened 51 H. glycines effectors to identify candidates that could inhibit effector‐triggered immunity (ETI) and/or pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI). We identified three effectors as ETI suppressors and seven effectors as PTI suppressors. We also assessed expression modulation of plant immune marker genes as a function of these suppressors. |
format | Online Article Text |
id | pubmed-7411561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74115612020-08-10 Screening soybean cyst nematode effectors for their ability to suppress plant immunity Pogorelko, Gennady Wang, Jianying Juvale, Parijat S. Mitchum, Melissa G. Baum, Thomas J. Mol Plant Pathol Short Communications The soybean cyst nematode (SCN), Heterodera glycines, is one of the most destructive pathogens of soybeans. SCN is an obligate and sedentary parasite that transforms host plant root cells into an elaborate permanent feeding site, a syncytium. Formation and maintenance of a viable syncytium is an absolute requirement for nematode growth and reproduction. In turn, sensing pathogen attack, plants activate defence responses and may trigger programmed cell death at the sites of infection. For successful parasitism, H. glycines must suppress these host defence responses to establish and maintain viable syncytia. Similar to other pathogens, H. glycines engages in these molecular interactions with its host via effector proteins. The goal of this study was to conduct a comprehensive screen to identify H. glycines effectors that interfere with plant immune responses. We used Nicotiana benthamiana plants infected by Pseudomonas syringae and Pseudomonas fluorescens strains. Using these pathosystems, we screened 51 H. glycines effectors to identify candidates that could inhibit effector‐triggered immunity (ETI) and/or pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI). We identified three effectors as ETI suppressors and seven effectors as PTI suppressors. We also assessed expression modulation of plant immune marker genes as a function of these suppressors. John Wiley and Sons Inc. 2020-07-16 /pmc/articles/PMC7411561/ /pubmed/32672422 http://dx.doi.org/10.1111/mpp.12972 Text en © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Short Communications Pogorelko, Gennady Wang, Jianying Juvale, Parijat S. Mitchum, Melissa G. Baum, Thomas J. Screening soybean cyst nematode effectors for their ability to suppress plant immunity |
title | Screening soybean cyst nematode effectors for their ability to suppress plant immunity |
title_full | Screening soybean cyst nematode effectors for their ability to suppress plant immunity |
title_fullStr | Screening soybean cyst nematode effectors for their ability to suppress plant immunity |
title_full_unstemmed | Screening soybean cyst nematode effectors for their ability to suppress plant immunity |
title_short | Screening soybean cyst nematode effectors for their ability to suppress plant immunity |
title_sort | screening soybean cyst nematode effectors for their ability to suppress plant immunity |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411561/ https://www.ncbi.nlm.nih.gov/pubmed/32672422 http://dx.doi.org/10.1111/mpp.12972 |
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