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Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean

Chitin, an N-acetylglucosamine polymer, is well-known to have unique biological functions, such as growth promotion and disease resistance induction in plants. Chitin has been expectedly used for improving crop yield using its functions; however, chitin derivatives, such as chitin oligosaccharide (C...

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Autores principales: Kaminaka, Hironori, Miura, Chihiro, Isowa, Yukiko, Tominaga, Takaya, Gonnami, Mamu, Egusa, Mayumi, Ifuku, Shinsuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411678/
https://www.ncbi.nlm.nih.gov/pubmed/32605205
http://dx.doi.org/10.3390/plants9070810
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author Kaminaka, Hironori
Miura, Chihiro
Isowa, Yukiko
Tominaga, Takaya
Gonnami, Mamu
Egusa, Mayumi
Ifuku, Shinsuke
author_facet Kaminaka, Hironori
Miura, Chihiro
Isowa, Yukiko
Tominaga, Takaya
Gonnami, Mamu
Egusa, Mayumi
Ifuku, Shinsuke
author_sort Kaminaka, Hironori
collection PubMed
description Chitin, an N-acetylglucosamine polymer, is well-known to have unique biological functions, such as growth promotion and disease resistance induction in plants. Chitin has been expectedly used for improving crop yield using its functions; however, chitin derivatives, such as chitin oligosaccharide (CO) and chitosan, are widely used instead since chitin is difficult to handle because of its insolubility. Chitin nanofiber (CNF), produced from chitin through nanofibrillation, retains its polymeric structure and can be dispersed uniformly even in water. Here, the effects of CO and CNF on plant responses were directly compared in soybeans (Glycine max) to define the most effective method to produce chitin derivatives for plant response induction. The growth promotion of aerial parts was observed only in CNF-treated plants. The transcriptome analysis showed that the number of differentially expressed genes (DEGs) in CNF-treated soybeans was higher than in CO-treated soybeans. Notably, the expression patterns of DEGs were mostly similar but were strongly induced by CNF treatment as compared with the CO group. These results reveal that CNF can induce stronger plant response to chitin than CO in soybeans, suggesting nanofibrillation, rather than oligomerization, as a more effective method to produce chitin derivatives for plant response induction.
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spelling pubmed-74116782020-08-25 Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean Kaminaka, Hironori Miura, Chihiro Isowa, Yukiko Tominaga, Takaya Gonnami, Mamu Egusa, Mayumi Ifuku, Shinsuke Plants (Basel) Article Chitin, an N-acetylglucosamine polymer, is well-known to have unique biological functions, such as growth promotion and disease resistance induction in plants. Chitin has been expectedly used for improving crop yield using its functions; however, chitin derivatives, such as chitin oligosaccharide (CO) and chitosan, are widely used instead since chitin is difficult to handle because of its insolubility. Chitin nanofiber (CNF), produced from chitin through nanofibrillation, retains its polymeric structure and can be dispersed uniformly even in water. Here, the effects of CO and CNF on plant responses were directly compared in soybeans (Glycine max) to define the most effective method to produce chitin derivatives for plant response induction. The growth promotion of aerial parts was observed only in CNF-treated plants. The transcriptome analysis showed that the number of differentially expressed genes (DEGs) in CNF-treated soybeans was higher than in CO-treated soybeans. Notably, the expression patterns of DEGs were mostly similar but were strongly induced by CNF treatment as compared with the CO group. These results reveal that CNF can induce stronger plant response to chitin than CO in soybeans, suggesting nanofibrillation, rather than oligomerization, as a more effective method to produce chitin derivatives for plant response induction. MDPI 2020-06-28 /pmc/articles/PMC7411678/ /pubmed/32605205 http://dx.doi.org/10.3390/plants9070810 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kaminaka, Hironori
Miura, Chihiro
Isowa, Yukiko
Tominaga, Takaya
Gonnami, Mamu
Egusa, Mayumi
Ifuku, Shinsuke
Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean
title Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean
title_full Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean
title_fullStr Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean
title_full_unstemmed Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean
title_short Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean
title_sort nanofibrillation is an effective method to produce chitin derivatives for induction of plant responses in soybean
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411678/
https://www.ncbi.nlm.nih.gov/pubmed/32605205
http://dx.doi.org/10.3390/plants9070810
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