Cargando…

Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas

Several oncolytic viruses (OVs) including various human and canine adenoviruses, canine distemper virus, herpes-simplex virus, reovirus, and members of the poxvirus family, such as vaccinia virus and myxoma virus, have been successfully tested for canine cancer therapy in preclinical and clinical se...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrov, Ivan, Gentschev, Ivaylo, Vyalkova, Anna, Elashry, Mohamed I., Klymiuk, Michele C., Arnhold, Stefan, Szalay, Aladar A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411685/
https://www.ncbi.nlm.nih.gov/pubmed/32664672
http://dx.doi.org/10.3390/v12070750
_version_ 1783568435487178752
author Petrov, Ivan
Gentschev, Ivaylo
Vyalkova, Anna
Elashry, Mohamed I.
Klymiuk, Michele C.
Arnhold, Stefan
Szalay, Aladar A.
author_facet Petrov, Ivan
Gentschev, Ivaylo
Vyalkova, Anna
Elashry, Mohamed I.
Klymiuk, Michele C.
Arnhold, Stefan
Szalay, Aladar A.
author_sort Petrov, Ivan
collection PubMed
description Several oncolytic viruses (OVs) including various human and canine adenoviruses, canine distemper virus, herpes-simplex virus, reovirus, and members of the poxvirus family, such as vaccinia virus and myxoma virus, have been successfully tested for canine cancer therapy in preclinical and clinical settings. The success of the cancer virotherapy is dependent on the ability of oncolytic viruses to overcome the attacks of the host immune system, to preferentially infect and lyse cancer cells, and to initiate tumor-specific immunity. To date, several different strategies have been developed to overcome the antiviral host defense barriers. In our study, we used canine adipose-derived mesenchymal stem cells (cAdMSCs) as a “Trojan horse” for the delivery of oncolytic vaccinia virus Copenhagen strain to achieve maximum oncolysis against canine soft tissue sarcoma (CSTS) tumors. A single systemic administration of vaccinia virus-loaded cAdMSCs was found to be safe and led to the significant reduction and substantial inhibition of tumor growth in a CSTS xenograft mouse model. This is the first example that vaccinia virus-loaded cAdMSCs could serve as a therapeutic agent against CSTS tumors.
format Online
Article
Text
id pubmed-7411685
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74116852020-08-25 Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas Petrov, Ivan Gentschev, Ivaylo Vyalkova, Anna Elashry, Mohamed I. Klymiuk, Michele C. Arnhold, Stefan Szalay, Aladar A. Viruses Article Several oncolytic viruses (OVs) including various human and canine adenoviruses, canine distemper virus, herpes-simplex virus, reovirus, and members of the poxvirus family, such as vaccinia virus and myxoma virus, have been successfully tested for canine cancer therapy in preclinical and clinical settings. The success of the cancer virotherapy is dependent on the ability of oncolytic viruses to overcome the attacks of the host immune system, to preferentially infect and lyse cancer cells, and to initiate tumor-specific immunity. To date, several different strategies have been developed to overcome the antiviral host defense barriers. In our study, we used canine adipose-derived mesenchymal stem cells (cAdMSCs) as a “Trojan horse” for the delivery of oncolytic vaccinia virus Copenhagen strain to achieve maximum oncolysis against canine soft tissue sarcoma (CSTS) tumors. A single systemic administration of vaccinia virus-loaded cAdMSCs was found to be safe and led to the significant reduction and substantial inhibition of tumor growth in a CSTS xenograft mouse model. This is the first example that vaccinia virus-loaded cAdMSCs could serve as a therapeutic agent against CSTS tumors. MDPI 2020-07-12 /pmc/articles/PMC7411685/ /pubmed/32664672 http://dx.doi.org/10.3390/v12070750 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Petrov, Ivan
Gentschev, Ivaylo
Vyalkova, Anna
Elashry, Mohamed I.
Klymiuk, Michele C.
Arnhold, Stefan
Szalay, Aladar A.
Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas
title Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas
title_full Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas
title_fullStr Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas
title_full_unstemmed Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas
title_short Canine Adipose-Derived Mesenchymal Stem Cells (cAdMSCs) as a “Trojan Horse” in Vaccinia Virus Mediated Oncolytic Therapy against Canine Soft Tissue Sarcomas
title_sort canine adipose-derived mesenchymal stem cells (cadmscs) as a “trojan horse” in vaccinia virus mediated oncolytic therapy against canine soft tissue sarcomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411685/
https://www.ncbi.nlm.nih.gov/pubmed/32664672
http://dx.doi.org/10.3390/v12070750
work_keys_str_mv AT petrovivan canineadiposederivedmesenchymalstemcellscadmscsasatrojanhorseinvacciniavirusmediatedoncolytictherapyagainstcaninesofttissuesarcomas
AT gentschevivaylo canineadiposederivedmesenchymalstemcellscadmscsasatrojanhorseinvacciniavirusmediatedoncolytictherapyagainstcaninesofttissuesarcomas
AT vyalkovaanna canineadiposederivedmesenchymalstemcellscadmscsasatrojanhorseinvacciniavirusmediatedoncolytictherapyagainstcaninesofttissuesarcomas
AT elashrymohamedi canineadiposederivedmesenchymalstemcellscadmscsasatrojanhorseinvacciniavirusmediatedoncolytictherapyagainstcaninesofttissuesarcomas
AT klymiukmichelec canineadiposederivedmesenchymalstemcellscadmscsasatrojanhorseinvacciniavirusmediatedoncolytictherapyagainstcaninesofttissuesarcomas
AT arnholdstefan canineadiposederivedmesenchymalstemcellscadmscsasatrojanhorseinvacciniavirusmediatedoncolytictherapyagainstcaninesofttissuesarcomas
AT szalayaladara canineadiposederivedmesenchymalstemcellscadmscsasatrojanhorseinvacciniavirusmediatedoncolytictherapyagainstcaninesofttissuesarcomas