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Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD
Panax vietnamensis (PV), a wild Panax species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little information on the accumulation of saponins i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411744/ https://www.ncbi.nlm.nih.gov/pubmed/32645855 http://dx.doi.org/10.3390/molecules25133086 |
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author | Vu-Huynh, Kim Long Nguyen, Huy Truong Van Le, Thi Hong Ma, Chi Thanh Lee, Gwang Jin Kwon, Sung Won Park, Jeong Hill Nguyen, Minh Duc |
author_facet | Vu-Huynh, Kim Long Nguyen, Huy Truong Van Le, Thi Hong Ma, Chi Thanh Lee, Gwang Jin Kwon, Sung Won Park, Jeong Hill Nguyen, Minh Duc |
author_sort | Vu-Huynh, Kim Long |
collection | PubMed |
description | Panax vietnamensis (PV), a wild Panax species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little information on the accumulation of saponins in PV during cultivation, but this information could serve as an indication of the appropriate harvest time. In this study we developed an HPLC-UV/ELSD method to simultaneously determine the content of 10 characteristic saponins in PV from 2–7 years old, including G-Rb1, G-Rd, G-Rg1, G-Re, N-R1, M-R1, M-R2, V-R2, V-R11, and p-RT4. The result indicated that from 2 to 5 years, the content of saponins in PV rhizome and radix increase 3.02 and 4.2 times, respectively, whereas from 5 to 7 years, no significant changes were observed. Hence, our study suggests that after 5 years of growth could be considered as an appropriate time for PV to be harvested. Among the analyzed saponins, G-Rg1, G-Rb1, G-Rd, and especially M-R2 were the major saponins that contributed to the change of PV’s saponin content through the years. In addition, the developed and validated HPLC method was proven to be reliable and effective for quality control of PV. |
format | Online Article Text |
id | pubmed-7411744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74117442020-08-25 Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD Vu-Huynh, Kim Long Nguyen, Huy Truong Van Le, Thi Hong Ma, Chi Thanh Lee, Gwang Jin Kwon, Sung Won Park, Jeong Hill Nguyen, Minh Duc Molecules Article Panax vietnamensis (PV), a wild Panax species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little information on the accumulation of saponins in PV during cultivation, but this information could serve as an indication of the appropriate harvest time. In this study we developed an HPLC-UV/ELSD method to simultaneously determine the content of 10 characteristic saponins in PV from 2–7 years old, including G-Rb1, G-Rd, G-Rg1, G-Re, N-R1, M-R1, M-R2, V-R2, V-R11, and p-RT4. The result indicated that from 2 to 5 years, the content of saponins in PV rhizome and radix increase 3.02 and 4.2 times, respectively, whereas from 5 to 7 years, no significant changes were observed. Hence, our study suggests that after 5 years of growth could be considered as an appropriate time for PV to be harvested. Among the analyzed saponins, G-Rg1, G-Rb1, G-Rd, and especially M-R2 were the major saponins that contributed to the change of PV’s saponin content through the years. In addition, the developed and validated HPLC method was proven to be reliable and effective for quality control of PV. MDPI 2020-07-07 /pmc/articles/PMC7411744/ /pubmed/32645855 http://dx.doi.org/10.3390/molecules25133086 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vu-Huynh, Kim Long Nguyen, Huy Truong Van Le, Thi Hong Ma, Chi Thanh Lee, Gwang Jin Kwon, Sung Won Park, Jeong Hill Nguyen, Minh Duc Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD |
title | Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD |
title_full | Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD |
title_fullStr | Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD |
title_full_unstemmed | Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD |
title_short | Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD |
title_sort | accumulation of saponins in underground parts of panax vietnamensis at different ages analyzed by hplc-uv/elsd |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411744/ https://www.ncbi.nlm.nih.gov/pubmed/32645855 http://dx.doi.org/10.3390/molecules25133086 |
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