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Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells

The aim of this study was to determine the anti-tumor activity of extracts isolated from Potentilla alba L. on human colon cancer cells of the HT-29 line and on non-cancer colon epithelial cells of the CCD 841 CoTr line. The research methods we used to determine the cytotoxic and proliferative prope...

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Autores principales: Kowalik, Klaudia, Paduch, Roman, Strawa, Jakub W., Wiater, Adrian, Wlizło, Kamila, Waśko, Adam, Wertel, Iwona, Pawłowska, Anna, Tomczykowa, Monika, Tomczyk, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411782/
https://www.ncbi.nlm.nih.gov/pubmed/32640760
http://dx.doi.org/10.3390/molecules25133080
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author Kowalik, Klaudia
Paduch, Roman
Strawa, Jakub W.
Wiater, Adrian
Wlizło, Kamila
Waśko, Adam
Wertel, Iwona
Pawłowska, Anna
Tomczykowa, Monika
Tomczyk, Michał
author_facet Kowalik, Klaudia
Paduch, Roman
Strawa, Jakub W.
Wiater, Adrian
Wlizło, Kamila
Waśko, Adam
Wertel, Iwona
Pawłowska, Anna
Tomczykowa, Monika
Tomczyk, Michał
author_sort Kowalik, Klaudia
collection PubMed
description The aim of this study was to determine the anti-tumor activity of extracts isolated from Potentilla alba L. on human colon cancer cells of the HT-29 line and on non-cancer colon epithelial cells of the CCD 841 CoTr line. The research methods we used to determine the cytotoxic and proliferative properties were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, the ability to produce nitric oxide, the Griess method, and the biochemical properties like 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods indicating reduction activity of tested samples. In order to obtain a phytochemical profile of the different extracts an analytical method based on liquid chromatography-photodiode array detection-electrospray ionization ion-trap time-of-flight mass spectrometry (LC-PDA-ESI-MS/TOF) was applied. Finally, the effects of the extracts on the morphology and cell counts were assessed by May–Grünwald–Giemsa staining. After a comprehensive analysis of all the experiments, the extracts were found to demonstrate cytotoxic properties, they stimulated the division of non-cancer cells, and they were able to scavenge free radicals. In the NR method, the cell viability dropped to approximately 80% compared to the control. In the MTT assay, tumor cell proliferation decreased to 9.5% compared to the control. Therefore, we concluded that this plant has medical potential.
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spelling pubmed-74117822020-08-25 Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells Kowalik, Klaudia Paduch, Roman Strawa, Jakub W. Wiater, Adrian Wlizło, Kamila Waśko, Adam Wertel, Iwona Pawłowska, Anna Tomczykowa, Monika Tomczyk, Michał Molecules Article The aim of this study was to determine the anti-tumor activity of extracts isolated from Potentilla alba L. on human colon cancer cells of the HT-29 line and on non-cancer colon epithelial cells of the CCD 841 CoTr line. The research methods we used to determine the cytotoxic and proliferative properties were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, the ability to produce nitric oxide, the Griess method, and the biochemical properties like 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods indicating reduction activity of tested samples. In order to obtain a phytochemical profile of the different extracts an analytical method based on liquid chromatography-photodiode array detection-electrospray ionization ion-trap time-of-flight mass spectrometry (LC-PDA-ESI-MS/TOF) was applied. Finally, the effects of the extracts on the morphology and cell counts were assessed by May–Grünwald–Giemsa staining. After a comprehensive analysis of all the experiments, the extracts were found to demonstrate cytotoxic properties, they stimulated the division of non-cancer cells, and they were able to scavenge free radicals. In the NR method, the cell viability dropped to approximately 80% compared to the control. In the MTT assay, tumor cell proliferation decreased to 9.5% compared to the control. Therefore, we concluded that this plant has medical potential. MDPI 2020-07-06 /pmc/articles/PMC7411782/ /pubmed/32640760 http://dx.doi.org/10.3390/molecules25133080 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kowalik, Klaudia
Paduch, Roman
Strawa, Jakub W.
Wiater, Adrian
Wlizło, Kamila
Waśko, Adam
Wertel, Iwona
Pawłowska, Anna
Tomczykowa, Monika
Tomczyk, Michał
Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells
title Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells
title_full Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells
title_fullStr Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells
title_full_unstemmed Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells
title_short Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells
title_sort potentilla alba extracts affect the viability and proliferation of non-cancerous and cancerous colon human epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411782/
https://www.ncbi.nlm.nih.gov/pubmed/32640760
http://dx.doi.org/10.3390/molecules25133080
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