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Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model

Ongoing Ebola virus disease outbreaks in the Democratic Republic of the Congo follow the largest recorded outbreak in Western Africa (2013–2016). To combat outbreaks, testing of medical countermeasures (therapeutics or vaccines) requires a well-defined, reproducible, animal model. Here we present Eb...

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Autores principales: Bennett, Richard S., Logue, James, Liu, David X., Reeder, Rebecca J., Janosko, Krisztina B., Perry, Donna L., Cooper, Timothy K., Byrum, Russell, Ragland, Danny, St. Claire, Marisa, Adams, Ricky, Burdette, Tracey L., Brady, Tyler M., Hadley, Kyra, Waters, M. Colin, Shim, Rebecca, Dowling, William, Qin, Jing, Crozier, Ian, Jahrling, Peter B., Hensley, Lisa E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411891/
https://www.ncbi.nlm.nih.gov/pubmed/32674252
http://dx.doi.org/10.3390/v12070753
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author Bennett, Richard S.
Logue, James
Liu, David X.
Reeder, Rebecca J.
Janosko, Krisztina B.
Perry, Donna L.
Cooper, Timothy K.
Byrum, Russell
Ragland, Danny
St. Claire, Marisa
Adams, Ricky
Burdette, Tracey L.
Brady, Tyler M.
Hadley, Kyra
Waters, M. Colin
Shim, Rebecca
Dowling, William
Qin, Jing
Crozier, Ian
Jahrling, Peter B.
Hensley, Lisa E.
author_facet Bennett, Richard S.
Logue, James
Liu, David X.
Reeder, Rebecca J.
Janosko, Krisztina B.
Perry, Donna L.
Cooper, Timothy K.
Byrum, Russell
Ragland, Danny
St. Claire, Marisa
Adams, Ricky
Burdette, Tracey L.
Brady, Tyler M.
Hadley, Kyra
Waters, M. Colin
Shim, Rebecca
Dowling, William
Qin, Jing
Crozier, Ian
Jahrling, Peter B.
Hensley, Lisa E.
author_sort Bennett, Richard S.
collection PubMed
description Ongoing Ebola virus disease outbreaks in the Democratic Republic of the Congo follow the largest recorded outbreak in Western Africa (2013–2016). To combat outbreaks, testing of medical countermeasures (therapeutics or vaccines) requires a well-defined, reproducible, animal model. Here we present Ebola virus disease kinetics in 24 Chinese-origin rhesus monkeys exposed intramuscularly to a highly characterized, commercially available Kikwit Ebola virus Filovirus Animal Non-Clinical Group (FANG) stock. Until reaching predetermined clinical disease endpoint criteria, six animals underwent anesthesia for repeated clinical sampling and were compared to six that did not. Groups of three animals were euthanized and necropsied on days 3, 4, 5, and 6 post-exposure, respectively. In addition, three uninfected animals served as controls. Here, we present detailed characterization of clinical and laboratory disease kinetics and complete blood counts, serum chemistries, Ebola virus titers, and disease kinetics for future medical countermeasure (MCM) study design and control data. We measured no statistical difference in hematology, chemistry values, or time to clinical endpoint in animals that were anesthetized for clinical sampling during the acute disease compared to those that were not.
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spelling pubmed-74118912020-08-25 Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model Bennett, Richard S. Logue, James Liu, David X. Reeder, Rebecca J. Janosko, Krisztina B. Perry, Donna L. Cooper, Timothy K. Byrum, Russell Ragland, Danny St. Claire, Marisa Adams, Ricky Burdette, Tracey L. Brady, Tyler M. Hadley, Kyra Waters, M. Colin Shim, Rebecca Dowling, William Qin, Jing Crozier, Ian Jahrling, Peter B. Hensley, Lisa E. Viruses Article Ongoing Ebola virus disease outbreaks in the Democratic Republic of the Congo follow the largest recorded outbreak in Western Africa (2013–2016). To combat outbreaks, testing of medical countermeasures (therapeutics or vaccines) requires a well-defined, reproducible, animal model. Here we present Ebola virus disease kinetics in 24 Chinese-origin rhesus monkeys exposed intramuscularly to a highly characterized, commercially available Kikwit Ebola virus Filovirus Animal Non-Clinical Group (FANG) stock. Until reaching predetermined clinical disease endpoint criteria, six animals underwent anesthesia for repeated clinical sampling and were compared to six that did not. Groups of three animals were euthanized and necropsied on days 3, 4, 5, and 6 post-exposure, respectively. In addition, three uninfected animals served as controls. Here, we present detailed characterization of clinical and laboratory disease kinetics and complete blood counts, serum chemistries, Ebola virus titers, and disease kinetics for future medical countermeasure (MCM) study design and control data. We measured no statistical difference in hematology, chemistry values, or time to clinical endpoint in animals that were anesthetized for clinical sampling during the acute disease compared to those that were not. MDPI 2020-07-14 /pmc/articles/PMC7411891/ /pubmed/32674252 http://dx.doi.org/10.3390/v12070753 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bennett, Richard S.
Logue, James
Liu, David X.
Reeder, Rebecca J.
Janosko, Krisztina B.
Perry, Donna L.
Cooper, Timothy K.
Byrum, Russell
Ragland, Danny
St. Claire, Marisa
Adams, Ricky
Burdette, Tracey L.
Brady, Tyler M.
Hadley, Kyra
Waters, M. Colin
Shim, Rebecca
Dowling, William
Qin, Jing
Crozier, Ian
Jahrling, Peter B.
Hensley, Lisa E.
Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model
title Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model
title_full Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model
title_fullStr Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model
title_full_unstemmed Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model
title_short Kikwit Ebola Virus Disease Progression in the Rhesus Monkey Animal Model
title_sort kikwit ebola virus disease progression in the rhesus monkey animal model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411891/
https://www.ncbi.nlm.nih.gov/pubmed/32674252
http://dx.doi.org/10.3390/v12070753
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