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Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani

Trichoderma is a filamentous fungus that is widely distributed in nature. As a biological control agent of agricultural pests, Trichoderma species have been widely studied in recent years. This study aimed to understand the inhibitory mechanism of Trichoderma virens ZT05 on Rhizoctonia solani throug...

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Autores principales: Halifu, Saiyaremu, Deng, Xun, Song, Xiaoshuang, Song, Ruiqing, Liang, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412022/
https://www.ncbi.nlm.nih.gov/pubmed/32707691
http://dx.doi.org/10.3390/plants9070912
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author Halifu, Saiyaremu
Deng, Xun
Song, Xiaoshuang
Song, Ruiqing
Liang, Xu
author_facet Halifu, Saiyaremu
Deng, Xun
Song, Xiaoshuang
Song, Ruiqing
Liang, Xu
author_sort Halifu, Saiyaremu
collection PubMed
description Trichoderma is a filamentous fungus that is widely distributed in nature. As a biological control agent of agricultural pests, Trichoderma species have been widely studied in recent years. This study aimed to understand the inhibitory mechanism of Trichoderma virens ZT05 on Rhizoctonia solani through the side-by-side culture of T. virens ZT05 and R. solani. To this end, we investigated the effect of volatile and nonvolatile metabolites of T. virens ZT05 on the mycelium growth and enzyme activity of R. solani and analyzed transcriptome data collected from side-by-side culture. T. virens ZT05 has a significant antagonistic effect against R. solani. The mycelium of T. virens ZT05 spirally wraps around and penetrates the mycelium of R. solani and inhibits the growth of R. solani. The volatile and nonvolatile metabolites of T. virens ZT05 have significant inhibitory effects on the growth of R. solani. The nonvolatile metabolites of T. virens ZT05 significantly affect the mycelium proteins of R. solani, including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), selenium-dependent glutathione peroxidase (GSH-Px), soluble proteins, and malondialdehyde (MDA). Twenty genes associated with hyperparasitism, including extracellular proteases, oligopeptide transporters, G-protein coupled receptors (GPCRs), chitinases, glucanases, and proteases were found to be upregulated during the antagonistic process between T. virens ZT05 and R. solani. Thirty genes related to antibiosis function, including tetracycline resistance proteins, reductases, the heat shock response, the oxidative stress response, ATP-binding cassette (ABC) efflux transporters, and multidrug resistance transporters, were found to be upregulated during the side-by-side culture of T. virens ZT05 and R. solani. T. virens ZT05 has a significant inhibitory effect on R. solani, and its mechanism of action is associated with hyperparasitism and antibiosis.
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spelling pubmed-74120222020-08-25 Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani Halifu, Saiyaremu Deng, Xun Song, Xiaoshuang Song, Ruiqing Liang, Xu Plants (Basel) Article Trichoderma is a filamentous fungus that is widely distributed in nature. As a biological control agent of agricultural pests, Trichoderma species have been widely studied in recent years. This study aimed to understand the inhibitory mechanism of Trichoderma virens ZT05 on Rhizoctonia solani through the side-by-side culture of T. virens ZT05 and R. solani. To this end, we investigated the effect of volatile and nonvolatile metabolites of T. virens ZT05 on the mycelium growth and enzyme activity of R. solani and analyzed transcriptome data collected from side-by-side culture. T. virens ZT05 has a significant antagonistic effect against R. solani. The mycelium of T. virens ZT05 spirally wraps around and penetrates the mycelium of R. solani and inhibits the growth of R. solani. The volatile and nonvolatile metabolites of T. virens ZT05 have significant inhibitory effects on the growth of R. solani. The nonvolatile metabolites of T. virens ZT05 significantly affect the mycelium proteins of R. solani, including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), selenium-dependent glutathione peroxidase (GSH-Px), soluble proteins, and malondialdehyde (MDA). Twenty genes associated with hyperparasitism, including extracellular proteases, oligopeptide transporters, G-protein coupled receptors (GPCRs), chitinases, glucanases, and proteases were found to be upregulated during the antagonistic process between T. virens ZT05 and R. solani. Thirty genes related to antibiosis function, including tetracycline resistance proteins, reductases, the heat shock response, the oxidative stress response, ATP-binding cassette (ABC) efflux transporters, and multidrug resistance transporters, were found to be upregulated during the side-by-side culture of T. virens ZT05 and R. solani. T. virens ZT05 has a significant inhibitory effect on R. solani, and its mechanism of action is associated with hyperparasitism and antibiosis. MDPI 2020-07-19 /pmc/articles/PMC7412022/ /pubmed/32707691 http://dx.doi.org/10.3390/plants9070912 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Halifu, Saiyaremu
Deng, Xun
Song, Xiaoshuang
Song, Ruiqing
Liang, Xu
Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
title Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
title_full Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
title_fullStr Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
title_full_unstemmed Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
title_short Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
title_sort inhibitory mechanism of trichoderma virens zt05 on rhizoctonia solani
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412022/
https://www.ncbi.nlm.nih.gov/pubmed/32707691
http://dx.doi.org/10.3390/plants9070912
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