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Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems

Organ-on-a-chip devices are gaining popularity in medical research due to the possibility of performing extremely complex living-body-resembling research in vitro. For this reason, there is a substantial drive in developing technologies capable of producing such structures in a simple and, at the sa...

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Autores principales: Bakhchova, Liubov, Jonušauskas, Linas, Andrijec, Dovilė, Kurachkina, Marharyta, Baravykas, Tomas, Eremin, Alexey, Steinmann, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412128/
https://www.ncbi.nlm.nih.gov/pubmed/32664211
http://dx.doi.org/10.3390/ma13143076
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author Bakhchova, Liubov
Jonušauskas, Linas
Andrijec, Dovilė
Kurachkina, Marharyta
Baravykas, Tomas
Eremin, Alexey
Steinmann, Ulrike
author_facet Bakhchova, Liubov
Jonušauskas, Linas
Andrijec, Dovilė
Kurachkina, Marharyta
Baravykas, Tomas
Eremin, Alexey
Steinmann, Ulrike
author_sort Bakhchova, Liubov
collection PubMed
description Organ-on-a-chip devices are gaining popularity in medical research due to the possibility of performing extremely complex living-body-resembling research in vitro. For this reason, there is a substantial drive in developing technologies capable of producing such structures in a simple and, at the same time, flexible manner. One of the primary challenges in producing organ-on-chip devices from a manufacturing standpoint is the prevalence of layer-by-layer bonding techniques, which result in limitations relating to the applicable materials and geometries and limited repeatability. In this work, we present an improved approach, using three dimensional (3D) laser lithography for the direct integration of a functional part—the membrane—into a closed-channel system. We show that it allows the freely choice of the geometry of the membrane and its integration into a complete organ-on-a-chip system. Considerations relating to sample preparation, the writing process, and the final preparation for operation are given. Overall, we consider that the broader application of 3D laser lithography in organ-on-a-chip fabrication is the next logical step in this field’s evolution.
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spelling pubmed-74121282020-08-25 Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems Bakhchova, Liubov Jonušauskas, Linas Andrijec, Dovilė Kurachkina, Marharyta Baravykas, Tomas Eremin, Alexey Steinmann, Ulrike Materials (Basel) Article Organ-on-a-chip devices are gaining popularity in medical research due to the possibility of performing extremely complex living-body-resembling research in vitro. For this reason, there is a substantial drive in developing technologies capable of producing such structures in a simple and, at the same time, flexible manner. One of the primary challenges in producing organ-on-chip devices from a manufacturing standpoint is the prevalence of layer-by-layer bonding techniques, which result in limitations relating to the applicable materials and geometries and limited repeatability. In this work, we present an improved approach, using three dimensional (3D) laser lithography for the direct integration of a functional part—the membrane—into a closed-channel system. We show that it allows the freely choice of the geometry of the membrane and its integration into a complete organ-on-a-chip system. Considerations relating to sample preparation, the writing process, and the final preparation for operation are given. Overall, we consider that the broader application of 3D laser lithography in organ-on-a-chip fabrication is the next logical step in this field’s evolution. MDPI 2020-07-10 /pmc/articles/PMC7412128/ /pubmed/32664211 http://dx.doi.org/10.3390/ma13143076 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bakhchova, Liubov
Jonušauskas, Linas
Andrijec, Dovilė
Kurachkina, Marharyta
Baravykas, Tomas
Eremin, Alexey
Steinmann, Ulrike
Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems
title Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems
title_full Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems
title_fullStr Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems
title_full_unstemmed Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems
title_short Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems
title_sort femtosecond laser-based integration of nano-membranes into organ-on-a-chip systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412128/
https://www.ncbi.nlm.nih.gov/pubmed/32664211
http://dx.doi.org/10.3390/ma13143076
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