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Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain

Bacteriophages are bacterial predators, which are garnering much interest nowadays vis-à-vis the global phenomenon of antimicrobial resistance. Bacteriophage preparations seem to be an alternative to antibiotics, which can be used at all levels of the food production chain. Their safety and efficacy...

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Autores principales: Wójcik, Ewelina A., Stańczyk, Małgorzata, Wojtasik, Arkadiusz, Kowalska, Justyna D., Nowakowska, Magdalena, Łukasiak, Magdalena, Bartnicka, Milena, Kazimierczak, Joanna, Dastych, Jarosław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412135/
https://www.ncbi.nlm.nih.gov/pubmed/32664206
http://dx.doi.org/10.3390/v12070742
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author Wójcik, Ewelina A.
Stańczyk, Małgorzata
Wojtasik, Arkadiusz
Kowalska, Justyna D.
Nowakowska, Magdalena
Łukasiak, Magdalena
Bartnicka, Milena
Kazimierczak, Joanna
Dastych, Jarosław
author_facet Wójcik, Ewelina A.
Stańczyk, Małgorzata
Wojtasik, Arkadiusz
Kowalska, Justyna D.
Nowakowska, Magdalena
Łukasiak, Magdalena
Bartnicka, Milena
Kazimierczak, Joanna
Dastych, Jarosław
author_sort Wójcik, Ewelina A.
collection PubMed
description Bacteriophages are bacterial predators, which are garnering much interest nowadays vis-à-vis the global phenomenon of antimicrobial resistance. Bacteriophage preparations seem to be an alternative to antibiotics, which can be used at all levels of the food production chain. Their safety and efficacy, however, are of public concern. In this study, a detailed evaluation of BAFASAL(®) preparation was performed. BAFASAL(®) is a bacteriophage cocktail that reduces Salmonella in poultry farming. In vivo acute and sub-chronic toxicity studies on rats and tolerance study on targeted animals (chicken broiler) conducted according to GLP and OECD guidelines did not reveal any signs of toxicity, which could be associated with BAFASAL(®) administration. In addition, no evidences of genotoxicity were observed. The tolerance study with 100-times concentrated dose also did not show any statistically significant differences in the assessed parameters. The in vitro crop assay, mimicking normal feed storage and feed application conditions showed that BAFASAL(®) reduced the number of Salmonella bacteria in experimentally contaminated feed. Moreover, reductions were observed for all examined forms (liquid, powder, spray). Furthermore, the in vivo efficacy study showed that treatment with BAFASAL(®) significantly decreased Salmonella content in caeca of birds infected with Salmonella Enteritidis. Detailed examination of BAFASAL(®) in terms of safety and efficacy, adds to the body of evidence that bacteriophages are harmless to animals and effective in the struggle against bacteria.
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spelling pubmed-74121352020-08-25 Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain Wójcik, Ewelina A. Stańczyk, Małgorzata Wojtasik, Arkadiusz Kowalska, Justyna D. Nowakowska, Magdalena Łukasiak, Magdalena Bartnicka, Milena Kazimierczak, Joanna Dastych, Jarosław Viruses Article Bacteriophages are bacterial predators, which are garnering much interest nowadays vis-à-vis the global phenomenon of antimicrobial resistance. Bacteriophage preparations seem to be an alternative to antibiotics, which can be used at all levels of the food production chain. Their safety and efficacy, however, are of public concern. In this study, a detailed evaluation of BAFASAL(®) preparation was performed. BAFASAL(®) is a bacteriophage cocktail that reduces Salmonella in poultry farming. In vivo acute and sub-chronic toxicity studies on rats and tolerance study on targeted animals (chicken broiler) conducted according to GLP and OECD guidelines did not reveal any signs of toxicity, which could be associated with BAFASAL(®) administration. In addition, no evidences of genotoxicity were observed. The tolerance study with 100-times concentrated dose also did not show any statistically significant differences in the assessed parameters. The in vitro crop assay, mimicking normal feed storage and feed application conditions showed that BAFASAL(®) reduced the number of Salmonella bacteria in experimentally contaminated feed. Moreover, reductions were observed for all examined forms (liquid, powder, spray). Furthermore, the in vivo efficacy study showed that treatment with BAFASAL(®) significantly decreased Salmonella content in caeca of birds infected with Salmonella Enteritidis. Detailed examination of BAFASAL(®) in terms of safety and efficacy, adds to the body of evidence that bacteriophages are harmless to animals and effective in the struggle against bacteria. MDPI 2020-07-10 /pmc/articles/PMC7412135/ /pubmed/32664206 http://dx.doi.org/10.3390/v12070742 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wójcik, Ewelina A.
Stańczyk, Małgorzata
Wojtasik, Arkadiusz
Kowalska, Justyna D.
Nowakowska, Magdalena
Łukasiak, Magdalena
Bartnicka, Milena
Kazimierczak, Joanna
Dastych, Jarosław
Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain
title Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain
title_full Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain
title_fullStr Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain
title_full_unstemmed Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain
title_short Comprehensive Evaluation of the Safety and Efficacy of BAFASAL(®) Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain
title_sort comprehensive evaluation of the safety and efficacy of bafasal(®) bacteriophage preparation for the reduction of salmonella in the food chain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412135/
https://www.ncbi.nlm.nih.gov/pubmed/32664206
http://dx.doi.org/10.3390/v12070742
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