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Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line

Dendropanax morbifera is a versatile plant that has been used as a herbal medicine due to its various useful medicinal effects. To protect its active component from biological stress and increase its drug efficacy as well as drug bioavailability, nanoemulsion was prepared. Dendropanax morbifera zinc...

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Autores principales: Rupa, Esrat Jahan, Arunkumar, Lakshminarayanan, Han, Yaxi, Kang, Jong Pyo, Ahn, Jong Chan, Jung, Seok-Kyu, Kim, Mia, Kim, Jong Yun, Yang, Deok-Chun, Lee, Gyong Jai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412136/
https://www.ncbi.nlm.nih.gov/pubmed/32709058
http://dx.doi.org/10.3390/ma13143197
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author Rupa, Esrat Jahan
Arunkumar, Lakshminarayanan
Han, Yaxi
Kang, Jong Pyo
Ahn, Jong Chan
Jung, Seok-Kyu
Kim, Mia
Kim, Jong Yun
Yang, Deok-Chun
Lee, Gyong Jai
author_facet Rupa, Esrat Jahan
Arunkumar, Lakshminarayanan
Han, Yaxi
Kang, Jong Pyo
Ahn, Jong Chan
Jung, Seok-Kyu
Kim, Mia
Kim, Jong Yun
Yang, Deok-Chun
Lee, Gyong Jai
author_sort Rupa, Esrat Jahan
collection PubMed
description Dendropanax morbifera is a versatile plant that has been used as a herbal medicine due to its various useful medicinal effects. To protect its active component from biological stress and increase its drug efficacy as well as drug bioavailability, nanoemulsion was prepared. Dendropanax morbifera zinc oxide nanoparticles (DM-ZnO NPs) were synthesized using the plant extract via the co-precipitation method and loaded with active indole-3-carbinol for nanoemulsion formulation using the ultrasonication process. Field emission transmission electron microscope revealed the flower shape of the Dendropanax morbifera indole-3-carbinol zinc oxide nanoemulsion (DM-ZnO-I3C-NE). In contrast, DM-ZnO NPs showed a spheroid shape that coincides agreeably with field emission electron scanning microscope. The hydrodynamic sizes by dynamic light scattering are about 65 ± 3 nm and 239.6 ± 6 nm and the crystallite sizes from X-ray diffraction are 11.52 nm and 16.07 nm for DM-ZnO NPs and DM-ZnO-I3C-NE, respectively. In vitro analysis revealed the cytotoxicity of DM-ZnO-I3C-NE against a human lung cancer cell line (A549) at 12.5 µg/mL as well as reactive oxygen species (ROS) production. The DM-ZnO-I3C-NE-induced ROS generation level was higher than that of DM-ZnO NPs and free indole-3-carbinol. The synergistic effect of DM-ZnO and indole-3-carbinol indicates DM-ZnO-I3C-NE as a potential candidate for future lung cancer drug and could be scope for functional food.
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spelling pubmed-74121362020-08-25 Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line Rupa, Esrat Jahan Arunkumar, Lakshminarayanan Han, Yaxi Kang, Jong Pyo Ahn, Jong Chan Jung, Seok-Kyu Kim, Mia Kim, Jong Yun Yang, Deok-Chun Lee, Gyong Jai Materials (Basel) Article Dendropanax morbifera is a versatile plant that has been used as a herbal medicine due to its various useful medicinal effects. To protect its active component from biological stress and increase its drug efficacy as well as drug bioavailability, nanoemulsion was prepared. Dendropanax morbifera zinc oxide nanoparticles (DM-ZnO NPs) were synthesized using the plant extract via the co-precipitation method and loaded with active indole-3-carbinol for nanoemulsion formulation using the ultrasonication process. Field emission transmission electron microscope revealed the flower shape of the Dendropanax morbifera indole-3-carbinol zinc oxide nanoemulsion (DM-ZnO-I3C-NE). In contrast, DM-ZnO NPs showed a spheroid shape that coincides agreeably with field emission electron scanning microscope. The hydrodynamic sizes by dynamic light scattering are about 65 ± 3 nm and 239.6 ± 6 nm and the crystallite sizes from X-ray diffraction are 11.52 nm and 16.07 nm for DM-ZnO NPs and DM-ZnO-I3C-NE, respectively. In vitro analysis revealed the cytotoxicity of DM-ZnO-I3C-NE against a human lung cancer cell line (A549) at 12.5 µg/mL as well as reactive oxygen species (ROS) production. The DM-ZnO-I3C-NE-induced ROS generation level was higher than that of DM-ZnO NPs and free indole-3-carbinol. The synergistic effect of DM-ZnO and indole-3-carbinol indicates DM-ZnO-I3C-NE as a potential candidate for future lung cancer drug and could be scope for functional food. MDPI 2020-07-17 /pmc/articles/PMC7412136/ /pubmed/32709058 http://dx.doi.org/10.3390/ma13143197 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rupa, Esrat Jahan
Arunkumar, Lakshminarayanan
Han, Yaxi
Kang, Jong Pyo
Ahn, Jong Chan
Jung, Seok-Kyu
Kim, Mia
Kim, Jong Yun
Yang, Deok-Chun
Lee, Gyong Jai
Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line
title Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line
title_full Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line
title_fullStr Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line
title_full_unstemmed Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line
title_short Dendropanax Morbifera Extract-Mediated ZnO Nanoparticles Loaded with Indole-3-Carbinol for Enhancement of Anticancer Efficacy in the A549 Human Lung Carcinoma Cell Line
title_sort dendropanax morbifera extract-mediated zno nanoparticles loaded with indole-3-carbinol for enhancement of anticancer efficacy in the a549 human lung carcinoma cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412136/
https://www.ncbi.nlm.nih.gov/pubmed/32709058
http://dx.doi.org/10.3390/ma13143197
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