Cargando…

Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis

The fungus Ascosphaera apis, an obligate fungal pathogen of honey bee brood, causes chalkbrood disease in honey bee larvae worldwide. Biological characteristics of the fungal pathogen and the molecular interactions between A. apis and honey bees have been studied extensively. However, little is know...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Zhiguo, Hou, Mengshang, Qiu, Yuanmei, Zhao, Bian, Nie, Hongyi, Su, Songkun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412215/
https://www.ncbi.nlm.nih.gov/pubmed/32640515
http://dx.doi.org/10.3390/insects11070419
_version_ 1783568556484460544
author Li, Zhiguo
Hou, Mengshang
Qiu, Yuanmei
Zhao, Bian
Nie, Hongyi
Su, Songkun
author_facet Li, Zhiguo
Hou, Mengshang
Qiu, Yuanmei
Zhao, Bian
Nie, Hongyi
Su, Songkun
author_sort Li, Zhiguo
collection PubMed
description The fungus Ascosphaera apis, an obligate fungal pathogen of honey bee brood, causes chalkbrood disease in honey bee larvae worldwide. Biological characteristics of the fungal pathogen and the molecular interactions between A. apis and honey bees have been studied extensively. However, little is known about the effects of A. apis infection on antioxidant enzyme activities and metabolic profiles of the gut of honey bee larvae. In this study, sandwich enzyme-linked immunosorbent assay and LC-MS based untargeted metabolomic analysis were employed to determine the changes in the specific activities of antioxidant enzymes and the metabolomic profiles in gut tissues of A. apis-infected larvae (10(5) A. apis spores per larva) and controls. Results showed that specific activities of superoxide dismutase, catalase and glutathione S-transferase were significantly higher in the guts of the control larvae than in the guts of the A. apis-infected larvae. The metabolomic data revealed that levels of 28 and 52 metabolites were significantly higher and lower, respectively, in the guts of A. apis-infected larvae than in the guts of control larvae. The 5-oxo-ETE level in the infected larvae was two times higher than that in the control larvae. Elevated 5-oxo-ETE levels may act as a potential metabolic biomarker for chalkbrood disease diagnosis, suggesting that A. apis infection induced obvious oxidative stress in the honey bee larvae. The levels of metabolites such as taurine, docosahexaenoic acid, and L-carnitine involved in combating oxidative stress were significantly decreased in the gut of A. apis-infected larvae. Overall, our results suggest that A. apis infection may compromise the ability of infected larvae to cope with oxidative stress, providing new insight into changing patterns of physiological responses to A. apis infection in honey bee larvae by concurrent use of conventional biochemical assays and untargeted metabolomics.
format Online
Article
Text
id pubmed-7412215
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74122152020-08-17 Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis Li, Zhiguo Hou, Mengshang Qiu, Yuanmei Zhao, Bian Nie, Hongyi Su, Songkun Insects Article The fungus Ascosphaera apis, an obligate fungal pathogen of honey bee brood, causes chalkbrood disease in honey bee larvae worldwide. Biological characteristics of the fungal pathogen and the molecular interactions between A. apis and honey bees have been studied extensively. However, little is known about the effects of A. apis infection on antioxidant enzyme activities and metabolic profiles of the gut of honey bee larvae. In this study, sandwich enzyme-linked immunosorbent assay and LC-MS based untargeted metabolomic analysis were employed to determine the changes in the specific activities of antioxidant enzymes and the metabolomic profiles in gut tissues of A. apis-infected larvae (10(5) A. apis spores per larva) and controls. Results showed that specific activities of superoxide dismutase, catalase and glutathione S-transferase were significantly higher in the guts of the control larvae than in the guts of the A. apis-infected larvae. The metabolomic data revealed that levels of 28 and 52 metabolites were significantly higher and lower, respectively, in the guts of A. apis-infected larvae than in the guts of control larvae. The 5-oxo-ETE level in the infected larvae was two times higher than that in the control larvae. Elevated 5-oxo-ETE levels may act as a potential metabolic biomarker for chalkbrood disease diagnosis, suggesting that A. apis infection induced obvious oxidative stress in the honey bee larvae. The levels of metabolites such as taurine, docosahexaenoic acid, and L-carnitine involved in combating oxidative stress were significantly decreased in the gut of A. apis-infected larvae. Overall, our results suggest that A. apis infection may compromise the ability of infected larvae to cope with oxidative stress, providing new insight into changing patterns of physiological responses to A. apis infection in honey bee larvae by concurrent use of conventional biochemical assays and untargeted metabolomics. MDPI 2020-07-06 /pmc/articles/PMC7412215/ /pubmed/32640515 http://dx.doi.org/10.3390/insects11070419 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Zhiguo
Hou, Mengshang
Qiu, Yuanmei
Zhao, Bian
Nie, Hongyi
Su, Songkun
Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis
title Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis
title_full Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis
title_fullStr Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis
title_full_unstemmed Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis
title_short Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis
title_sort changes in antioxidant enzymes activity and metabolomic profiles in the guts of honey bee (apis mellifera) larvae infected with ascosphaera apis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412215/
https://www.ncbi.nlm.nih.gov/pubmed/32640515
http://dx.doi.org/10.3390/insects11070419
work_keys_str_mv AT lizhiguo changesinantioxidantenzymesactivityandmetabolomicprofilesinthegutsofhoneybeeapismelliferalarvaeinfectedwithascosphaeraapis
AT houmengshang changesinantioxidantenzymesactivityandmetabolomicprofilesinthegutsofhoneybeeapismelliferalarvaeinfectedwithascosphaeraapis
AT qiuyuanmei changesinantioxidantenzymesactivityandmetabolomicprofilesinthegutsofhoneybeeapismelliferalarvaeinfectedwithascosphaeraapis
AT zhaobian changesinantioxidantenzymesactivityandmetabolomicprofilesinthegutsofhoneybeeapismelliferalarvaeinfectedwithascosphaeraapis
AT niehongyi changesinantioxidantenzymesactivityandmetabolomicprofilesinthegutsofhoneybeeapismelliferalarvaeinfectedwithascosphaeraapis
AT susongkun changesinantioxidantenzymesactivityandmetabolomicprofilesinthegutsofhoneybeeapismelliferalarvaeinfectedwithascosphaeraapis