Cargando…

The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells

Microglial cells clear the brain of pathogens and harmful debris, including amyloid-β (Aβ) deposits that are formed during Alzheimer’s disease (AD). We studied the expression of Msr1, Ager and Cd36 receptors involved in Aβ uptake and expression of Cd33 protein, which is considered a risk factor in A...

Descripción completa

Detalles Bibliográficos
Autores principales: Sikorska, Katarzyna, Grądzka, Iwona, Wasyk, Iwona, Brzóska, Kamil, Stępkowski, Tomasz M., Czerwińska, Malwina, Kruszewski, Marcin K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412234/
https://www.ncbi.nlm.nih.gov/pubmed/32698417
http://dx.doi.org/10.3390/ma13143227
_version_ 1783568560969220096
author Sikorska, Katarzyna
Grądzka, Iwona
Wasyk, Iwona
Brzóska, Kamil
Stępkowski, Tomasz M.
Czerwińska, Malwina
Kruszewski, Marcin K.
author_facet Sikorska, Katarzyna
Grądzka, Iwona
Wasyk, Iwona
Brzóska, Kamil
Stępkowski, Tomasz M.
Czerwińska, Malwina
Kruszewski, Marcin K.
author_sort Sikorska, Katarzyna
collection PubMed
description Microglial cells clear the brain of pathogens and harmful debris, including amyloid-β (Aβ) deposits that are formed during Alzheimer’s disease (AD). We studied the expression of Msr1, Ager and Cd36 receptors involved in Aβ uptake and expression of Cd33 protein, which is considered a risk factor in AD. The effect of silver nanoparticles (AgNP) and cadmium telluride quantum dots (CdTeQD) on the expression of the above receptors and Aβ uptake by microglial cells was investigated. Absorption of Aβ and NP was confirmed by confocal microscopy. AgNP, but not CdTeQD, caused a decrease in Aβ accumulation. By using a specific inhibitor—polyinosinic acid—we demonstrated that Aβ and AgNP compete for scavenger receptors. Real-time PCR showed up-regulation of Cd33 and Cd36 gene expression after treatment with CdTeQD for 24 h. Analysis of the abundance of the receptors on the cell surface revealed that AgNP treatment significantly reduced the presence of Msr1, Cd33, Ager and Cd36 receptors (6 and 24 h), whereas CdTeQD increased the levels of Msr1 and Cd36 (24 h). To summarize, we showed that AgNP uptake competes with Aβ uptake by microglial cells and consequently can impair the removal of the aggregates. In turn, CdTeQD treatment led to the accumulation of proinflammatory Cd36 protein on the cell surface.
format Online
Article
Text
id pubmed-7412234
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74122342020-08-17 The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells Sikorska, Katarzyna Grądzka, Iwona Wasyk, Iwona Brzóska, Kamil Stępkowski, Tomasz M. Czerwińska, Malwina Kruszewski, Marcin K. Materials (Basel) Article Microglial cells clear the brain of pathogens and harmful debris, including amyloid-β (Aβ) deposits that are formed during Alzheimer’s disease (AD). We studied the expression of Msr1, Ager and Cd36 receptors involved in Aβ uptake and expression of Cd33 protein, which is considered a risk factor in AD. The effect of silver nanoparticles (AgNP) and cadmium telluride quantum dots (CdTeQD) on the expression of the above receptors and Aβ uptake by microglial cells was investigated. Absorption of Aβ and NP was confirmed by confocal microscopy. AgNP, but not CdTeQD, caused a decrease in Aβ accumulation. By using a specific inhibitor—polyinosinic acid—we demonstrated that Aβ and AgNP compete for scavenger receptors. Real-time PCR showed up-regulation of Cd33 and Cd36 gene expression after treatment with CdTeQD for 24 h. Analysis of the abundance of the receptors on the cell surface revealed that AgNP treatment significantly reduced the presence of Msr1, Cd33, Ager and Cd36 receptors (6 and 24 h), whereas CdTeQD increased the levels of Msr1 and Cd36 (24 h). To summarize, we showed that AgNP uptake competes with Aβ uptake by microglial cells and consequently can impair the removal of the aggregates. In turn, CdTeQD treatment led to the accumulation of proinflammatory Cd36 protein on the cell surface. MDPI 2020-07-20 /pmc/articles/PMC7412234/ /pubmed/32698417 http://dx.doi.org/10.3390/ma13143227 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sikorska, Katarzyna
Grądzka, Iwona
Wasyk, Iwona
Brzóska, Kamil
Stępkowski, Tomasz M.
Czerwińska, Malwina
Kruszewski, Marcin K.
The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells
title The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells
title_full The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells
title_fullStr The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells
title_full_unstemmed The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells
title_short The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells
title_sort impact of ag nanoparticles and cdte quantum dots on expression and function of receptors involved in amyloid-β uptake by bv-2 microglial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412234/
https://www.ncbi.nlm.nih.gov/pubmed/32698417
http://dx.doi.org/10.3390/ma13143227
work_keys_str_mv AT sikorskakatarzyna theimpactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT gradzkaiwona theimpactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT wasykiwona theimpactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT brzoskakamil theimpactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT stepkowskitomaszm theimpactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT czerwinskamalwina theimpactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT kruszewskimarcink theimpactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT sikorskakatarzyna impactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT gradzkaiwona impactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT wasykiwona impactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT brzoskakamil impactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT stepkowskitomaszm impactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT czerwinskamalwina impactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells
AT kruszewskimarcink impactofagnanoparticlesandcdtequantumdotsonexpressionandfunctionofreceptorsinvolvedinamyloidbuptakebybv2microglialcells