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Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles

Propolis natural extracts have been used since ancient times due to their antioxidant, anti-inflammatory, antiviral, and antimicrobial activities. In this study, we produced scaffolds of type I collagen, extracted from Wistar Hanover rat tail tendons, and impregnated them with propolis nanoparticles...

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Autores principales: González-Masís, Jeimmy, Cubero-Sesin, Jorge M., Corrales-Ureña, Yendry R., González-Camacho, Sara, Mora-Ugalde, Nohelia, Baizán-Rojas, Mónica, Loaiza, Randall, Vega-Baudrit, José Roberto, Gonzalez-Paz, Rodolfo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412245/
https://www.ncbi.nlm.nih.gov/pubmed/32668654
http://dx.doi.org/10.3390/ma13143118
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author González-Masís, Jeimmy
Cubero-Sesin, Jorge M.
Corrales-Ureña, Yendry R.
González-Camacho, Sara
Mora-Ugalde, Nohelia
Baizán-Rojas, Mónica
Loaiza, Randall
Vega-Baudrit, José Roberto
Gonzalez-Paz, Rodolfo J.
author_facet González-Masís, Jeimmy
Cubero-Sesin, Jorge M.
Corrales-Ureña, Yendry R.
González-Camacho, Sara
Mora-Ugalde, Nohelia
Baizán-Rojas, Mónica
Loaiza, Randall
Vega-Baudrit, José Roberto
Gonzalez-Paz, Rodolfo J.
author_sort González-Masís, Jeimmy
collection PubMed
description Propolis natural extracts have been used since ancient times due to their antioxidant, anti-inflammatory, antiviral, and antimicrobial activities. In this study, we produced scaffolds of type I collagen, extracted from Wistar Hanover rat tail tendons, and impregnated them with propolis nanoparticles (NPs) for applications in regenerative medicine. Our results show that the impregnation of propolis NPs to collagen scaffolds affected the collagen denaturation temperature and tensile strength. The changes in structural collagen self-assembly due to contact with organic nanoparticles were shown for the first time. The fibril collagen secondary structure was preserved, and the D-pattern gap increased to 135 ± 28 nm, without losing the microfiber structure. We also show that the properties of the collagen scaffolds depended on the concentration of propolis NPs. A concentration of 100 μg/mL of propolis NPs with 1 mg of collagen, with a hydrodynamic diameter of 173 nm, was found to be an optimal concentration to enhance 3T3 fibroblast cell metabolic activity and cell proliferation. The expected outcome from this research is both scientifically and socially relevant since the home scaffold using natural nanoparticles can be produced using a simple method and could be widely used for local medical care in developing communities.
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spelling pubmed-74122452020-08-17 Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles González-Masís, Jeimmy Cubero-Sesin, Jorge M. Corrales-Ureña, Yendry R. González-Camacho, Sara Mora-Ugalde, Nohelia Baizán-Rojas, Mónica Loaiza, Randall Vega-Baudrit, José Roberto Gonzalez-Paz, Rodolfo J. Materials (Basel) Article Propolis natural extracts have been used since ancient times due to their antioxidant, anti-inflammatory, antiviral, and antimicrobial activities. In this study, we produced scaffolds of type I collagen, extracted from Wistar Hanover rat tail tendons, and impregnated them with propolis nanoparticles (NPs) for applications in regenerative medicine. Our results show that the impregnation of propolis NPs to collagen scaffolds affected the collagen denaturation temperature and tensile strength. The changes in structural collagen self-assembly due to contact with organic nanoparticles were shown for the first time. The fibril collagen secondary structure was preserved, and the D-pattern gap increased to 135 ± 28 nm, without losing the microfiber structure. We also show that the properties of the collagen scaffolds depended on the concentration of propolis NPs. A concentration of 100 μg/mL of propolis NPs with 1 mg of collagen, with a hydrodynamic diameter of 173 nm, was found to be an optimal concentration to enhance 3T3 fibroblast cell metabolic activity and cell proliferation. The expected outcome from this research is both scientifically and socially relevant since the home scaffold using natural nanoparticles can be produced using a simple method and could be widely used for local medical care in developing communities. MDPI 2020-07-13 /pmc/articles/PMC7412245/ /pubmed/32668654 http://dx.doi.org/10.3390/ma13143118 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
González-Masís, Jeimmy
Cubero-Sesin, Jorge M.
Corrales-Ureña, Yendry R.
González-Camacho, Sara
Mora-Ugalde, Nohelia
Baizán-Rojas, Mónica
Loaiza, Randall
Vega-Baudrit, José Roberto
Gonzalez-Paz, Rodolfo J.
Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles
title Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles
title_full Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles
title_fullStr Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles
title_full_unstemmed Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles
title_short Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles
title_sort increased fibroblast metabolic activity of collagen scaffolds via the addition of propolis nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412245/
https://www.ncbi.nlm.nih.gov/pubmed/32668654
http://dx.doi.org/10.3390/ma13143118
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