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Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression

Microbiome management is a promising way to suppress verticillium wilt, a severe disease in Brassica caused by Verticillium longisporum. In order to improve current biocontrol strategies, we compared bacterial Verticillium antagonists in different assays using a hierarchical selection and evaluation...

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Autores principales: Rybakova, Daria, Wikström, Mariann, Birch-Jensen, Fia, Postma, Joeke, Ehlers, Ralf Udo, Schmuck, Maria, Kollmann, René, Köhl, Jürgen, Berg, Gabriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412322/
https://www.ncbi.nlm.nih.gov/pubmed/32650549
http://dx.doi.org/10.3390/plants9070866
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author Rybakova, Daria
Wikström, Mariann
Birch-Jensen, Fia
Postma, Joeke
Ehlers, Ralf Udo
Schmuck, Maria
Kollmann, René
Köhl, Jürgen
Berg, Gabriele
author_facet Rybakova, Daria
Wikström, Mariann
Birch-Jensen, Fia
Postma, Joeke
Ehlers, Ralf Udo
Schmuck, Maria
Kollmann, René
Köhl, Jürgen
Berg, Gabriele
author_sort Rybakova, Daria
collection PubMed
description Microbiome management is a promising way to suppress verticillium wilt, a severe disease in Brassica caused by Verticillium longisporum. In order to improve current biocontrol strategies, we compared bacterial Verticillium antagonists in different assays using a hierarchical selection and evaluation scheme, and we integrated outcomes of our previous studies. The result was strongly dependent on the assessment method chosen (in vitro, in vivo, in situ), on the growth conditions of the plants and their genotype. The most promising biocontrol candidate identified was a Brassica endophyte Serratia plymuthica F20. Positive results were confirmed in field trials and by microscopically visualizing the three-way interaction. Applying antagonists in seed treatment contributes to an exceptionally low ecological footprint, supporting efficient economic and ecological solutions to controlling verticillium wilt. Indigenous microbiome, especially soil and seed microbiome, has been identified as key to understanding disease outbreaks and suppression. We suggest that verticillium wilt is a microbiome-driven disease caused by a reduction in microbial diversity within seeds and in the soil surrounding them. We strongly recommend integrating microbiome data in the development of new biocontrol and breeding strategies and combining both strategies with the aim of designing healthy microbiomes, thus making plants more resilient toward soil-borne pathogens.
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spelling pubmed-74123222020-08-17 Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression Rybakova, Daria Wikström, Mariann Birch-Jensen, Fia Postma, Joeke Ehlers, Ralf Udo Schmuck, Maria Kollmann, René Köhl, Jürgen Berg, Gabriele Plants (Basel) Article Microbiome management is a promising way to suppress verticillium wilt, a severe disease in Brassica caused by Verticillium longisporum. In order to improve current biocontrol strategies, we compared bacterial Verticillium antagonists in different assays using a hierarchical selection and evaluation scheme, and we integrated outcomes of our previous studies. The result was strongly dependent on the assessment method chosen (in vitro, in vivo, in situ), on the growth conditions of the plants and their genotype. The most promising biocontrol candidate identified was a Brassica endophyte Serratia plymuthica F20. Positive results were confirmed in field trials and by microscopically visualizing the three-way interaction. Applying antagonists in seed treatment contributes to an exceptionally low ecological footprint, supporting efficient economic and ecological solutions to controlling verticillium wilt. Indigenous microbiome, especially soil and seed microbiome, has been identified as key to understanding disease outbreaks and suppression. We suggest that verticillium wilt is a microbiome-driven disease caused by a reduction in microbial diversity within seeds and in the soil surrounding them. We strongly recommend integrating microbiome data in the development of new biocontrol and breeding strategies and combining both strategies with the aim of designing healthy microbiomes, thus making plants more resilient toward soil-borne pathogens. MDPI 2020-07-08 /pmc/articles/PMC7412322/ /pubmed/32650549 http://dx.doi.org/10.3390/plants9070866 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rybakova, Daria
Wikström, Mariann
Birch-Jensen, Fia
Postma, Joeke
Ehlers, Ralf Udo
Schmuck, Maria
Kollmann, René
Köhl, Jürgen
Berg, Gabriele
Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression
title Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression
title_full Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression
title_fullStr Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression
title_full_unstemmed Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression
title_short Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression
title_sort verticillium wilt in oilseed rape—the microbiome is crucial for disease outbreaks as well as for efficient suppression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412322/
https://www.ncbi.nlm.nih.gov/pubmed/32650549
http://dx.doi.org/10.3390/plants9070866
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