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Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana

In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of...

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Detalles Bibliográficos
Autores principales: Shiva, Sunitha, Samarakoon, Thilani, Lowe, Kaleb A., Roach, Charles, Vu, Hieu Sy, Colter, Madeline, Porras, Hollie, Hwang, Caroline, Roth, Mary R., Tamura, Pamela, Li, Maoyin, Schrick, Kathrin, Shah, Jyoti, Wang, Xuemin, Wang, Haiyan, Welti, Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412450/
https://www.ncbi.nlm.nih.gov/pubmed/32635518
http://dx.doi.org/10.3390/plants9070845
Descripción
Sumario:In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of galactolipids, increases in phosphatidic acid and triacylglycerols, and formation of sterol glucosides and acyl sterol glucosides, have been observed more recently. In this work, by measuring lipid levels with mass spectrometry, we confirm the previously observed changes in Arabidopsis thaliana leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. We also observed increased levels of digalactosylmonoacylglycerols and monogalactosylmonoacylglycerols. The hypothesis that a defect in sterol glycosylation would adversely affect regrowth of plants after a severe heat stress regimen was tested, but differences between wild-type and sterol glycosylation-defective plants were not detected.