Cargando…
Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana
In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412450/ https://www.ncbi.nlm.nih.gov/pubmed/32635518 http://dx.doi.org/10.3390/plants9070845 |
_version_ | 1783568610769240064 |
---|---|
author | Shiva, Sunitha Samarakoon, Thilani Lowe, Kaleb A. Roach, Charles Vu, Hieu Sy Colter, Madeline Porras, Hollie Hwang, Caroline Roth, Mary R. Tamura, Pamela Li, Maoyin Schrick, Kathrin Shah, Jyoti Wang, Xuemin Wang, Haiyan Welti, Ruth |
author_facet | Shiva, Sunitha Samarakoon, Thilani Lowe, Kaleb A. Roach, Charles Vu, Hieu Sy Colter, Madeline Porras, Hollie Hwang, Caroline Roth, Mary R. Tamura, Pamela Li, Maoyin Schrick, Kathrin Shah, Jyoti Wang, Xuemin Wang, Haiyan Welti, Ruth |
author_sort | Shiva, Sunitha |
collection | PubMed |
description | In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of galactolipids, increases in phosphatidic acid and triacylglycerols, and formation of sterol glucosides and acyl sterol glucosides, have been observed more recently. In this work, by measuring lipid levels with mass spectrometry, we confirm the previously observed changes in Arabidopsis thaliana leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. We also observed increased levels of digalactosylmonoacylglycerols and monogalactosylmonoacylglycerols. The hypothesis that a defect in sterol glycosylation would adversely affect regrowth of plants after a severe heat stress regimen was tested, but differences between wild-type and sterol glycosylation-defective plants were not detected. |
format | Online Article Text |
id | pubmed-7412450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74124502020-08-26 Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana Shiva, Sunitha Samarakoon, Thilani Lowe, Kaleb A. Roach, Charles Vu, Hieu Sy Colter, Madeline Porras, Hollie Hwang, Caroline Roth, Mary R. Tamura, Pamela Li, Maoyin Schrick, Kathrin Shah, Jyoti Wang, Xuemin Wang, Haiyan Welti, Ruth Plants (Basel) Article In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of galactolipids, increases in phosphatidic acid and triacylglycerols, and formation of sterol glucosides and acyl sterol glucosides, have been observed more recently. In this work, by measuring lipid levels with mass spectrometry, we confirm the previously observed changes in Arabidopsis thaliana leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. We also observed increased levels of digalactosylmonoacylglycerols and monogalactosylmonoacylglycerols. The hypothesis that a defect in sterol glycosylation would adversely affect regrowth of plants after a severe heat stress regimen was tested, but differences between wild-type and sterol glycosylation-defective plants were not detected. MDPI 2020-07-04 /pmc/articles/PMC7412450/ /pubmed/32635518 http://dx.doi.org/10.3390/plants9070845 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shiva, Sunitha Samarakoon, Thilani Lowe, Kaleb A. Roach, Charles Vu, Hieu Sy Colter, Madeline Porras, Hollie Hwang, Caroline Roth, Mary R. Tamura, Pamela Li, Maoyin Schrick, Kathrin Shah, Jyoti Wang, Xuemin Wang, Haiyan Welti, Ruth Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana |
title | Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana |
title_full | Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana |
title_fullStr | Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana |
title_full_unstemmed | Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana |
title_short | Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana |
title_sort | leaf lipid alterations in response to heat stress of arabidopsis thaliana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412450/ https://www.ncbi.nlm.nih.gov/pubmed/32635518 http://dx.doi.org/10.3390/plants9070845 |
work_keys_str_mv | AT shivasunitha leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT samarakoonthilani leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT lowekaleba leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT roachcharles leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT vuhieusy leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT coltermadeline leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT porrashollie leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT hwangcaroline leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT rothmaryr leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT tamurapamela leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT limaoyin leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT schrickkathrin leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT shahjyoti leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT wangxuemin leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT wanghaiyan leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana AT weltiruth leaflipidalterationsinresponsetoheatstressofarabidopsisthaliana |