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Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions

Papillomaviruses (PVs) usually cause benign proliferative lesions in the stratified epithelium of various animal species. However, some high-risk types of PVs have been proven to lead to malignant transformations. In dogs, several canine papillomaviruses (CPVs) have been identified in malignant lesi...

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Autores principales: Chang, Chia-Yu, Yamashita-Kawanishi, Nanako, Tomizawa, Sonoka, Liu, I-Li, Chen, Wei-Tao, Chang, Yen-Chen, Huang, Wei-Hsiang, Tsai, Pei-Shiue, Shirota, Kinji, Chambers, James K, Uchida, Kazuyuki, Haga, Takeshi, Chang, Hui-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412457/
https://www.ncbi.nlm.nih.gov/pubmed/32650357
http://dx.doi.org/10.3390/v12070736
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author Chang, Chia-Yu
Yamashita-Kawanishi, Nanako
Tomizawa, Sonoka
Liu, I-Li
Chen, Wei-Tao
Chang, Yen-Chen
Huang, Wei-Hsiang
Tsai, Pei-Shiue
Shirota, Kinji
Chambers, James K
Uchida, Kazuyuki
Haga, Takeshi
Chang, Hui-Wen
author_facet Chang, Chia-Yu
Yamashita-Kawanishi, Nanako
Tomizawa, Sonoka
Liu, I-Li
Chen, Wei-Tao
Chang, Yen-Chen
Huang, Wei-Hsiang
Tsai, Pei-Shiue
Shirota, Kinji
Chambers, James K
Uchida, Kazuyuki
Haga, Takeshi
Chang, Hui-Wen
author_sort Chang, Chia-Yu
collection PubMed
description Papillomaviruses (PVs) usually cause benign proliferative lesions in the stratified epithelium of various animal species. However, some high-risk types of PVs have been proven to lead to malignant transformations. In dogs, several canine papillomaviruses (CPVs) have been identified in malignant lesions and are suggested as one of the risk factors for the development of squamous cell carcinomas (SCCs). In the present study, the full genomes of two CPV9 strains from recurrent SCCs of Dog 1 and skin viral papilloma (viral plaque) of Dog 2 were sequenced. Alignment of the two CPV9 sequences with the genome of the reference CPV9 strain (accession no. JF800656.1) derived from a solitary pigmented plaque was performed. Compared with the reference strain, a 27 bp in-frame insertion in the E1 gene was identified in both CPV9 strains in this study. In comparison with the CPV9 strains derived from benign lesions, the CPV9 from the SCCs of Dog 1 exhibited a 328 bp deletion at the 3′ end of the E2 and spacer sequence, which encoded a truncated deduced E2 protein and a chimeric E8^E2 protein. However, there was no difference in the mRNA expression levels of viral oncoproteins of E6 and E7 between the two CPV9 cases, suggesting that the oncogenesis of CPV9 for malignant transformation might be different from that of human papillomaviruses. The roles of E2 and E8^E2 deleted CPV9 in the oncogenesis of benign and malignant lesions should be further investigated.
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spelling pubmed-74124572020-08-26 Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions Chang, Chia-Yu Yamashita-Kawanishi, Nanako Tomizawa, Sonoka Liu, I-Li Chen, Wei-Tao Chang, Yen-Chen Huang, Wei-Hsiang Tsai, Pei-Shiue Shirota, Kinji Chambers, James K Uchida, Kazuyuki Haga, Takeshi Chang, Hui-Wen Viruses Article Papillomaviruses (PVs) usually cause benign proliferative lesions in the stratified epithelium of various animal species. However, some high-risk types of PVs have been proven to lead to malignant transformations. In dogs, several canine papillomaviruses (CPVs) have been identified in malignant lesions and are suggested as one of the risk factors for the development of squamous cell carcinomas (SCCs). In the present study, the full genomes of two CPV9 strains from recurrent SCCs of Dog 1 and skin viral papilloma (viral plaque) of Dog 2 were sequenced. Alignment of the two CPV9 sequences with the genome of the reference CPV9 strain (accession no. JF800656.1) derived from a solitary pigmented plaque was performed. Compared with the reference strain, a 27 bp in-frame insertion in the E1 gene was identified in both CPV9 strains in this study. In comparison with the CPV9 strains derived from benign lesions, the CPV9 from the SCCs of Dog 1 exhibited a 328 bp deletion at the 3′ end of the E2 and spacer sequence, which encoded a truncated deduced E2 protein and a chimeric E8^E2 protein. However, there was no difference in the mRNA expression levels of viral oncoproteins of E6 and E7 between the two CPV9 cases, suggesting that the oncogenesis of CPV9 for malignant transformation might be different from that of human papillomaviruses. The roles of E2 and E8^E2 deleted CPV9 in the oncogenesis of benign and malignant lesions should be further investigated. MDPI 2020-07-08 /pmc/articles/PMC7412457/ /pubmed/32650357 http://dx.doi.org/10.3390/v12070736 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chang, Chia-Yu
Yamashita-Kawanishi, Nanako
Tomizawa, Sonoka
Liu, I-Li
Chen, Wei-Tao
Chang, Yen-Chen
Huang, Wei-Hsiang
Tsai, Pei-Shiue
Shirota, Kinji
Chambers, James K
Uchida, Kazuyuki
Haga, Takeshi
Chang, Hui-Wen
Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions
title Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions
title_full Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions
title_fullStr Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions
title_full_unstemmed Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions
title_short Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions
title_sort whole genomic analysis and comparison of two canine papillomavirus type 9 strains in malignant and benign skin lesions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412457/
https://www.ncbi.nlm.nih.gov/pubmed/32650357
http://dx.doi.org/10.3390/v12070736
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