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Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use

In this work, synthesis and investigations on chitosan-based hydrogels modified with Aloe vera juice are presented. These materials were synthesized by UV radiation. Investigations involved analysis of chemical structure by FTIR spectroscopy, sorption properties in physiological liquids, strength pr...

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Autores principales: Drabczyk, Anna, Kudłacik-Kramarczyk, Sonia, Głąb, Magdalena, Kędzierska, Magdalena, Jaromin, Anna, Mierzwiński, Dariusz, Tyliszczak, Bożena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412484/
https://www.ncbi.nlm.nih.gov/pubmed/32660077
http://dx.doi.org/10.3390/ma13143073
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author Drabczyk, Anna
Kudłacik-Kramarczyk, Sonia
Głąb, Magdalena
Kędzierska, Magdalena
Jaromin, Anna
Mierzwiński, Dariusz
Tyliszczak, Bożena
author_facet Drabczyk, Anna
Kudłacik-Kramarczyk, Sonia
Głąb, Magdalena
Kędzierska, Magdalena
Jaromin, Anna
Mierzwiński, Dariusz
Tyliszczak, Bożena
author_sort Drabczyk, Anna
collection PubMed
description In this work, synthesis and investigations on chitosan-based hydrogels modified with Aloe vera juice are presented. These materials were synthesized by UV radiation. Investigations involved analysis of chemical structure by FTIR spectroscopy, sorption properties in physiological liquids, strength properties by texture analyzer, surface topography by Atomic Force Microscopy (AFM technique), and in vitro cytotoxicity by MTT test using L929 murine fibroblasts. Particular attention was focused both on determining the impact of the amount and the molecular weight of the crosslinker used for the synthesis as well as on the introduced additive on the properties of hydrogels. It was proven that modified hydrogels exhibited higher swelling ability. Introduced additive affected the tensile strength of hydrogels—modified materials showed 23% higher elongation. The greater amount of the crosslinker used in the synthesis, the more compact the structure, leading to the lower elasticity and lower sorption of hydrogels was reported. Above 95%, murine fibroblasts remained viable after 24 h incubation with hydrogels. It indicates that tested materials did not exhibit cytotoxicity toward these lines. Additionally, materials with Aloe vera juice were characterized by lower surface roughness. Conducted investigations allowed us to state that such modified hydrogels may be considered as useful for biomedical purposes.
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spelling pubmed-74124842020-08-26 Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use Drabczyk, Anna Kudłacik-Kramarczyk, Sonia Głąb, Magdalena Kędzierska, Magdalena Jaromin, Anna Mierzwiński, Dariusz Tyliszczak, Bożena Materials (Basel) Article In this work, synthesis and investigations on chitosan-based hydrogels modified with Aloe vera juice are presented. These materials were synthesized by UV radiation. Investigations involved analysis of chemical structure by FTIR spectroscopy, sorption properties in physiological liquids, strength properties by texture analyzer, surface topography by Atomic Force Microscopy (AFM technique), and in vitro cytotoxicity by MTT test using L929 murine fibroblasts. Particular attention was focused both on determining the impact of the amount and the molecular weight of the crosslinker used for the synthesis as well as on the introduced additive on the properties of hydrogels. It was proven that modified hydrogels exhibited higher swelling ability. Introduced additive affected the tensile strength of hydrogels—modified materials showed 23% higher elongation. The greater amount of the crosslinker used in the synthesis, the more compact the structure, leading to the lower elasticity and lower sorption of hydrogels was reported. Above 95%, murine fibroblasts remained viable after 24 h incubation with hydrogels. It indicates that tested materials did not exhibit cytotoxicity toward these lines. Additionally, materials with Aloe vera juice were characterized by lower surface roughness. Conducted investigations allowed us to state that such modified hydrogels may be considered as useful for biomedical purposes. MDPI 2020-07-09 /pmc/articles/PMC7412484/ /pubmed/32660077 http://dx.doi.org/10.3390/ma13143073 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Drabczyk, Anna
Kudłacik-Kramarczyk, Sonia
Głąb, Magdalena
Kędzierska, Magdalena
Jaromin, Anna
Mierzwiński, Dariusz
Tyliszczak, Bożena
Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use
title Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use
title_full Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use
title_fullStr Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use
title_full_unstemmed Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use
title_short Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use
title_sort physicochemical investigations of chitosan-based hydrogels containing aloe vera designed for biomedical use
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412484/
https://www.ncbi.nlm.nih.gov/pubmed/32660077
http://dx.doi.org/10.3390/ma13143073
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