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In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells

3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated...

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Autores principales: Cao, Shuaishuai, Han, Jonghyeuk, Sharma, Neha, Msallem, Bilal, Jeong, Wonwoo, Son, Jeonghyun, Kunz, Christoph, Kang, Hyun-Wook, Thieringer, Florian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412522/
https://www.ncbi.nlm.nih.gov/pubmed/32650530
http://dx.doi.org/10.3390/ma13143057
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author Cao, Shuaishuai
Han, Jonghyeuk
Sharma, Neha
Msallem, Bilal
Jeong, Wonwoo
Son, Jeonghyun
Kunz, Christoph
Kang, Hyun-Wook
Thieringer, Florian M.
author_facet Cao, Shuaishuai
Han, Jonghyeuk
Sharma, Neha
Msallem, Bilal
Jeong, Wonwoo
Son, Jeonghyun
Kunz, Christoph
Kang, Hyun-Wook
Thieringer, Florian M.
author_sort Cao, Shuaishuai
collection PubMed
description 3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated 3D poly (lactic-co-glycolic acid)/β-tricalcium phosphate (3D-PLGA/TCP) and 3D β-tricalcium phosphate (3D-TCP) scaffolds using two relatively distinct 3D printing (3DP) technologies. Conjunctively, we compared and investigated mechanical and biological responses on human dental pulp stem cells (hDPSCs). Physicochemical properties of the scaffolds, including pore structure, chemical elements, and compression modulus, were characterized. hDPSCs were cultured on scaffolds for subsequent investigations of biocompatibility and osteoconductivity. Our findings indicate that 3D printed PLGA/TCP and β-tricalcium phosphate (β-TCP) scaffolds possessed a highly interconnected and porous structure. 3D-TCP scaffolds exhibited better compressive strength than 3D-PLGA/TCP scaffolds, while the 3D-PLGA/TCP scaffolds revealed a flexible mechanical performance. The introduction of 3D structure and β-TCP components increased the adhesion and proliferation of hDPSCs and promoted osteogenic differentiation. In conclusion, 3D-PLGA/TCP and 3D-TCP scaffolds, with the incorporation of hDPSCs as a personalized restoration approach, has a prospective potential to repair minor and critical bone defects in oral and maxillofacial surgery, respectively.
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spelling pubmed-74125222020-08-26 In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells Cao, Shuaishuai Han, Jonghyeuk Sharma, Neha Msallem, Bilal Jeong, Wonwoo Son, Jeonghyun Kunz, Christoph Kang, Hyun-Wook Thieringer, Florian M. Materials (Basel) Article 3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated 3D poly (lactic-co-glycolic acid)/β-tricalcium phosphate (3D-PLGA/TCP) and 3D β-tricalcium phosphate (3D-TCP) scaffolds using two relatively distinct 3D printing (3DP) technologies. Conjunctively, we compared and investigated mechanical and biological responses on human dental pulp stem cells (hDPSCs). Physicochemical properties of the scaffolds, including pore structure, chemical elements, and compression modulus, were characterized. hDPSCs were cultured on scaffolds for subsequent investigations of biocompatibility and osteoconductivity. Our findings indicate that 3D printed PLGA/TCP and β-tricalcium phosphate (β-TCP) scaffolds possessed a highly interconnected and porous structure. 3D-TCP scaffolds exhibited better compressive strength than 3D-PLGA/TCP scaffolds, while the 3D-PLGA/TCP scaffolds revealed a flexible mechanical performance. The introduction of 3D structure and β-TCP components increased the adhesion and proliferation of hDPSCs and promoted osteogenic differentiation. In conclusion, 3D-PLGA/TCP and 3D-TCP scaffolds, with the incorporation of hDPSCs as a personalized restoration approach, has a prospective potential to repair minor and critical bone defects in oral and maxillofacial surgery, respectively. MDPI 2020-07-08 /pmc/articles/PMC7412522/ /pubmed/32650530 http://dx.doi.org/10.3390/ma13143057 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cao, Shuaishuai
Han, Jonghyeuk
Sharma, Neha
Msallem, Bilal
Jeong, Wonwoo
Son, Jeonghyun
Kunz, Christoph
Kang, Hyun-Wook
Thieringer, Florian M.
In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells
title In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells
title_full In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells
title_fullStr In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells
title_full_unstemmed In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells
title_short In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells
title_sort in vitro mechanical and biological properties of 3d printed polymer composite and β-tricalcium phosphate scaffold on human dental pulp stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412522/
https://www.ncbi.nlm.nih.gov/pubmed/32650530
http://dx.doi.org/10.3390/ma13143057
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