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Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum

Thoracic ossification of the ligamentum flavum (TOLF) causes serious spinal canal stenosis. The underlying aetiology may relate to genetic and inflammatory factors. DNA methylation plays a critical role in osteogenesis and inflammation, whereas there is no genome‐wide DNA methylation analysis about...

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Autores principales: Fan, Tianqi, Meng, Xiangyu, Sun, Chuiguo, Yang, Xiaoxi, Chen, Guanghui, Li, Weishi, Chen, Zhongqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412700/
https://www.ncbi.nlm.nih.gov/pubmed/32583558
http://dx.doi.org/10.1111/jcmm.15509
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author Fan, Tianqi
Meng, Xiangyu
Sun, Chuiguo
Yang, Xiaoxi
Chen, Guanghui
Li, Weishi
Chen, Zhongqiang
author_facet Fan, Tianqi
Meng, Xiangyu
Sun, Chuiguo
Yang, Xiaoxi
Chen, Guanghui
Li, Weishi
Chen, Zhongqiang
author_sort Fan, Tianqi
collection PubMed
description Thoracic ossification of the ligamentum flavum (TOLF) causes serious spinal canal stenosis. The underlying aetiology may relate to genetic and inflammatory factors. DNA methylation plays a critical role in osteogenesis and inflammation, whereas there is no genome‐wide DNA methylation analysis about TOLF. The two subtypes of TOLF (single‐level and multiple‐level) have distinct clinical features. Using micro‐computed tomography (micro‐CT), we showed the ossification arose from the joint between two vertebrae at one/both sides of ligament flavum. With Illumina Infinium Human Methylation 850 BeadChip arrays, genome‐wide DNA methylation profile was measured in ligament flavum of eight healthy and eight TOLF samples. Only 65 of the differentially methylated cytosine‐phosphate‐guanine dinucleotides were found in both subtype groups. Principal component analysis and heat map analysis showed a different methylation pattern in TOLF samples, and methylation patterns of two subtypes are also distinct. The Gene Ontology enrichment analysis was significantly enriched in differentiation and inflammation. Pyrosequencing analysis and quantitative real‐time polymerase chain reaction were performed to validate the arrays results and expression levels, to test six differentially methylated genes (SLC7A11, HOXA10, HOXA11AS, TNIK, homeobox transcript antisense RNA, IFITM1), using another independent samples (P < 0.05). Our findings first demonstrated an altered Genome‐wide DNA methylation profile in TOLF, and implied distinct methylated features in two subtypes.
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spelling pubmed-74127002020-08-10 Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum Fan, Tianqi Meng, Xiangyu Sun, Chuiguo Yang, Xiaoxi Chen, Guanghui Li, Weishi Chen, Zhongqiang J Cell Mol Med Original Articles Thoracic ossification of the ligamentum flavum (TOLF) causes serious spinal canal stenosis. The underlying aetiology may relate to genetic and inflammatory factors. DNA methylation plays a critical role in osteogenesis and inflammation, whereas there is no genome‐wide DNA methylation analysis about TOLF. The two subtypes of TOLF (single‐level and multiple‐level) have distinct clinical features. Using micro‐computed tomography (micro‐CT), we showed the ossification arose from the joint between two vertebrae at one/both sides of ligament flavum. With Illumina Infinium Human Methylation 850 BeadChip arrays, genome‐wide DNA methylation profile was measured in ligament flavum of eight healthy and eight TOLF samples. Only 65 of the differentially methylated cytosine‐phosphate‐guanine dinucleotides were found in both subtype groups. Principal component analysis and heat map analysis showed a different methylation pattern in TOLF samples, and methylation patterns of two subtypes are also distinct. The Gene Ontology enrichment analysis was significantly enriched in differentiation and inflammation. Pyrosequencing analysis and quantitative real‐time polymerase chain reaction were performed to validate the arrays results and expression levels, to test six differentially methylated genes (SLC7A11, HOXA10, HOXA11AS, TNIK, homeobox transcript antisense RNA, IFITM1), using another independent samples (P < 0.05). Our findings first demonstrated an altered Genome‐wide DNA methylation profile in TOLF, and implied distinct methylated features in two subtypes. John Wiley and Sons Inc. 2020-06-24 2020-08 /pmc/articles/PMC7412700/ /pubmed/32583558 http://dx.doi.org/10.1111/jcmm.15509 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Fan, Tianqi
Meng, Xiangyu
Sun, Chuiguo
Yang, Xiaoxi
Chen, Guanghui
Li, Weishi
Chen, Zhongqiang
Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum
title Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum
title_full Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum
title_fullStr Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum
title_full_unstemmed Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum
title_short Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum
title_sort genome‐wide dna methylation profile analysis in thoracic ossification of the ligamentum flavum
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412700/
https://www.ncbi.nlm.nih.gov/pubmed/32583558
http://dx.doi.org/10.1111/jcmm.15509
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