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Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA
The follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA) are malignant and benign thyroid neoplasms, respectively. MicroRNA (miRNA) expressions have been touted as an indicator for prognostic outcome in thyroid cancer. The study objective was to explore genes suppressed by miRNA-2...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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SAGE Publications
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412895/ https://www.ncbi.nlm.nih.gov/pubmed/32821081 http://dx.doi.org/10.1177/1176935120948474 |
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author | Nwadiugwu, Martin C |
author_facet | Nwadiugwu, Martin C |
author_sort | Nwadiugwu, Martin C |
collection | PubMed |
description | The follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA) are malignant and benign thyroid neoplasms, respectively. MicroRNA (miRNA) expressions have been touted as an indicator for prognostic outcome in thyroid cancer. The study objective was to explore genes suppressed by miRNA-21-3p and miRNA-21-5p for potential therapeutic insights. Differentially expressed genes and their functional enrichment were obtained from 25 FTA and 27 FTC gene microarray dataset GSE82208 using R and Bioconductor tools. The miRNA target sites were obtained from miR-TarBase database. A unique gene list of differentially expressed FTC and FTA were entered into miR-TarBase database to obtain target genes for both miRNA-21-3p and miRNA-21-5p. The result showed that miRNA-21-3p and miRNA-21-5p downregulated TIMP3, MAT2A, TGFBR2, and PLAT gene in FTC and FTA leading to significant expression of acute phase-response to metallothionein, metal ions, and unfolded protein response (UPR). The computational analysis suggests that the suppression of miRNA-21-3p and miRNA-21-5p could be an intervention strategy for therapeutically targeting FTC and FTA treatments. |
format | Online Article Text |
id | pubmed-7412895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-74128952020-08-19 Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA Nwadiugwu, Martin C Cancer Inform Original Research The follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA) are malignant and benign thyroid neoplasms, respectively. MicroRNA (miRNA) expressions have been touted as an indicator for prognostic outcome in thyroid cancer. The study objective was to explore genes suppressed by miRNA-21-3p and miRNA-21-5p for potential therapeutic insights. Differentially expressed genes and their functional enrichment were obtained from 25 FTA and 27 FTC gene microarray dataset GSE82208 using R and Bioconductor tools. The miRNA target sites were obtained from miR-TarBase database. A unique gene list of differentially expressed FTC and FTA were entered into miR-TarBase database to obtain target genes for both miRNA-21-3p and miRNA-21-5p. The result showed that miRNA-21-3p and miRNA-21-5p downregulated TIMP3, MAT2A, TGFBR2, and PLAT gene in FTC and FTA leading to significant expression of acute phase-response to metallothionein, metal ions, and unfolded protein response (UPR). The computational analysis suggests that the suppression of miRNA-21-3p and miRNA-21-5p could be an intervention strategy for therapeutically targeting FTC and FTA treatments. SAGE Publications 2020-08-05 /pmc/articles/PMC7412895/ /pubmed/32821081 http://dx.doi.org/10.1177/1176935120948474 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Nwadiugwu, Martin C Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA |
title | Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA |
title_full | Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA |
title_fullStr | Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA |
title_full_unstemmed | Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA |
title_short | Thyroid Tumor: Investigating MicroRNA-21 Gene Suppression in FTC and FTA |
title_sort | thyroid tumor: investigating microrna-21 gene suppression in ftc and fta |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412895/ https://www.ncbi.nlm.nih.gov/pubmed/32821081 http://dx.doi.org/10.1177/1176935120948474 |
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