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New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life”
The African baobab (Adansonia digitata L.), also referred to as the “Tree of Life”, is a majestic, long-lived and multipurpose tree of sub-Saharan Africa. Internationally, a growing demand for baobab products in the food, pharmaceutical and cosmetics industries has been observed. Considering this, t...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413363/ https://www.ncbi.nlm.nih.gov/pubmed/32764541 http://dx.doi.org/10.1038/s41598-020-68697-6 |
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author | Islam-Faridi, Nurul Sakhanokho, Hamidou F. Dana Nelson, C. |
author_facet | Islam-Faridi, Nurul Sakhanokho, Hamidou F. Dana Nelson, C. |
author_sort | Islam-Faridi, Nurul |
collection | PubMed |
description | The African baobab (Adansonia digitata L.), also referred to as the “Tree of Life”, is a majestic, long-lived and multipurpose tree of sub-Saharan Africa. Internationally, a growing demand for baobab products in the food, pharmaceutical and cosmetics industries has been observed. Considering this, there is a need for scientific information on the genetics and breeding of A. digitata, including cytogenetics, genetic diversity and reproductive biology. The objectives of our cytogenetic research were to determine the genome size, chromosome number, and organization of ribosomal DNA (45S and 5SrDNA) of A. digitata. Flow cytometry analysis revealed a 2C-DNA value of 3.8 ± 0.6 pg (1Cx monoploid genome size 919.1 ± 62.9 Mbp). Using our improved chromosome preparation technique, we were able to unequivocally count the chromosomes resulting in 2n = 4x = 168, a revised chromosome number for A. digitata. Fluorescent in situ hybridization (FISH) analysis revealed two massively large variants of 45S rDNA and their corresponding nucleolus organizer regions (NOR). The NOR variants were about two to four times larger than the main body of their respective chromosomes. To our knowledge, this is the first report of this phenomenon in a plant species. Furthermore, we found that FISH analysis using the Arabidopsis-type telomere repeat sequence probe clarified and confirmed the new chromosome number and characterized the 45S rDNA structural organization. |
format | Online Article Text |
id | pubmed-7413363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74133632020-08-10 New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life” Islam-Faridi, Nurul Sakhanokho, Hamidou F. Dana Nelson, C. Sci Rep Article The African baobab (Adansonia digitata L.), also referred to as the “Tree of Life”, is a majestic, long-lived and multipurpose tree of sub-Saharan Africa. Internationally, a growing demand for baobab products in the food, pharmaceutical and cosmetics industries has been observed. Considering this, there is a need for scientific information on the genetics and breeding of A. digitata, including cytogenetics, genetic diversity and reproductive biology. The objectives of our cytogenetic research were to determine the genome size, chromosome number, and organization of ribosomal DNA (45S and 5SrDNA) of A. digitata. Flow cytometry analysis revealed a 2C-DNA value of 3.8 ± 0.6 pg (1Cx monoploid genome size 919.1 ± 62.9 Mbp). Using our improved chromosome preparation technique, we were able to unequivocally count the chromosomes resulting in 2n = 4x = 168, a revised chromosome number for A. digitata. Fluorescent in situ hybridization (FISH) analysis revealed two massively large variants of 45S rDNA and their corresponding nucleolus organizer regions (NOR). The NOR variants were about two to four times larger than the main body of their respective chromosomes. To our knowledge, this is the first report of this phenomenon in a plant species. Furthermore, we found that FISH analysis using the Arabidopsis-type telomere repeat sequence probe clarified and confirmed the new chromosome number and characterized the 45S rDNA structural organization. Nature Publishing Group UK 2020-08-06 /pmc/articles/PMC7413363/ /pubmed/32764541 http://dx.doi.org/10.1038/s41598-020-68697-6 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Islam-Faridi, Nurul Sakhanokho, Hamidou F. Dana Nelson, C. New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life” |
title | New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life” |
title_full | New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life” |
title_fullStr | New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life” |
title_full_unstemmed | New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life” |
title_short | New chromosome number and cyto-molecular characterization of the African Baobab (Adansonia digitata L.) - “The Tree of Life” |
title_sort | new chromosome number and cyto-molecular characterization of the african baobab (adansonia digitata l.) - “the tree of life” |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413363/ https://www.ncbi.nlm.nih.gov/pubmed/32764541 http://dx.doi.org/10.1038/s41598-020-68697-6 |
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