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High-throughput investigation of molecular and cellular biomarkers in NMOSD

OBJECTIVE: To identify candidate biomarkers associated with neuromyelitis optica spectrum disorder (NMOSD) using high-throughput technologies that broadly assay the concentrations of serum analytes and frequencies of immune cell subsets. METHODS: Sera, peripheral blood mononuclear cells (PBMCs), and...

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Autores principales: Yandamuri, Soumya S., Jiang, Ruoyi, Sharma, Aditi, Cotzomi, Elizabeth, Zografou, Chrysoula, Ma, Anthony K., Alvey, Jessica S., Cook, Lawrence J., Smith, Terry J., Yeaman, Michael R., O'Connor, Kevin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413712/
https://www.ncbi.nlm.nih.gov/pubmed/32753407
http://dx.doi.org/10.1212/NXI.0000000000000852
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author Yandamuri, Soumya S.
Jiang, Ruoyi
Sharma, Aditi
Cotzomi, Elizabeth
Zografou, Chrysoula
Ma, Anthony K.
Alvey, Jessica S.
Cook, Lawrence J.
Smith, Terry J.
Yeaman, Michael R.
O'Connor, Kevin C.
author_facet Yandamuri, Soumya S.
Jiang, Ruoyi
Sharma, Aditi
Cotzomi, Elizabeth
Zografou, Chrysoula
Ma, Anthony K.
Alvey, Jessica S.
Cook, Lawrence J.
Smith, Terry J.
Yeaman, Michael R.
O'Connor, Kevin C.
author_sort Yandamuri, Soumya S.
collection PubMed
description OBJECTIVE: To identify candidate biomarkers associated with neuromyelitis optica spectrum disorder (NMOSD) using high-throughput technologies that broadly assay the concentrations of serum analytes and frequencies of immune cell subsets. METHODS: Sera, peripheral blood mononuclear cells (PBMCs), and matched clinical data from participants with NMOSD and healthy controls (HCs) were obtained from the Collaborative International Research in Clinical and Longitudinal Experience Study NMOSD biorepository. Flow cytometry panels were used to measure the frequencies of 39 T-cell, B-cell, regulatory T-cell, monocyte, natural killer (NK) cell, and dendritic cell subsets in unstimulated PBMCs. In parallel, multiplex proteomics assays were used to measure 46 serum cytokines and chemokines in 2 independent NMOSD and HC cohorts. Multivariable regression models were used to assess molecular and cellular profiles in NMOSD compared with HC. RESULTS: NMOSD samples had a lower frequency of CD16(+)CD56(+) NK cells. Both serum cohorts and multivariable logistic regression revealed increased levels of B-cell activating factor associated with NMOSD. Interleukin 6, CCL22, and CCL3 were also elevated in 1 NMOSD cohort of the 2 analyzed. Multivariable linear regression of serum analyte levels revealed a correlation between CX3CL1 (fractalkine) levels and the number of days since most recent disease relapse. CONCLUSIONS: Integrative analyses of cytokines, chemokines, and immune cells in participants with NMOSD and HCs provide congruence with previously identified biomarkers of NMOSD and highlight CD16(+)CD56(+) NK cells and CX3CL1 as potential novel biomarker candidates.
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spelling pubmed-74137122020-08-14 High-throughput investigation of molecular and cellular biomarkers in NMOSD Yandamuri, Soumya S. Jiang, Ruoyi Sharma, Aditi Cotzomi, Elizabeth Zografou, Chrysoula Ma, Anthony K. Alvey, Jessica S. Cook, Lawrence J. Smith, Terry J. Yeaman, Michael R. O'Connor, Kevin C. Neurol Neuroimmunol Neuroinflamm Article OBJECTIVE: To identify candidate biomarkers associated with neuromyelitis optica spectrum disorder (NMOSD) using high-throughput technologies that broadly assay the concentrations of serum analytes and frequencies of immune cell subsets. METHODS: Sera, peripheral blood mononuclear cells (PBMCs), and matched clinical data from participants with NMOSD and healthy controls (HCs) were obtained from the Collaborative International Research in Clinical and Longitudinal Experience Study NMOSD biorepository. Flow cytometry panels were used to measure the frequencies of 39 T-cell, B-cell, regulatory T-cell, monocyte, natural killer (NK) cell, and dendritic cell subsets in unstimulated PBMCs. In parallel, multiplex proteomics assays were used to measure 46 serum cytokines and chemokines in 2 independent NMOSD and HC cohorts. Multivariable regression models were used to assess molecular and cellular profiles in NMOSD compared with HC. RESULTS: NMOSD samples had a lower frequency of CD16(+)CD56(+) NK cells. Both serum cohorts and multivariable logistic regression revealed increased levels of B-cell activating factor associated with NMOSD. Interleukin 6, CCL22, and CCL3 were also elevated in 1 NMOSD cohort of the 2 analyzed. Multivariable linear regression of serum analyte levels revealed a correlation between CX3CL1 (fractalkine) levels and the number of days since most recent disease relapse. CONCLUSIONS: Integrative analyses of cytokines, chemokines, and immune cells in participants with NMOSD and HCs provide congruence with previously identified biomarkers of NMOSD and highlight CD16(+)CD56(+) NK cells and CX3CL1 as potential novel biomarker candidates. Lippincott Williams & Wilkins 2020-08-04 /pmc/articles/PMC7413712/ /pubmed/32753407 http://dx.doi.org/10.1212/NXI.0000000000000852 Text en Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Article
Yandamuri, Soumya S.
Jiang, Ruoyi
Sharma, Aditi
Cotzomi, Elizabeth
Zografou, Chrysoula
Ma, Anthony K.
Alvey, Jessica S.
Cook, Lawrence J.
Smith, Terry J.
Yeaman, Michael R.
O'Connor, Kevin C.
High-throughput investigation of molecular and cellular biomarkers in NMOSD
title High-throughput investigation of molecular and cellular biomarkers in NMOSD
title_full High-throughput investigation of molecular and cellular biomarkers in NMOSD
title_fullStr High-throughput investigation of molecular and cellular biomarkers in NMOSD
title_full_unstemmed High-throughput investigation of molecular and cellular biomarkers in NMOSD
title_short High-throughput investigation of molecular and cellular biomarkers in NMOSD
title_sort high-throughput investigation of molecular and cellular biomarkers in nmosd
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413712/
https://www.ncbi.nlm.nih.gov/pubmed/32753407
http://dx.doi.org/10.1212/NXI.0000000000000852
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