Cargando…

Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice

OBJECTIVE: Gestational diabetes mellitus (GDM) is a pathological condition, affecting an increasing number of pregnant women worldwide. Safe and effective treatment for GDM is very important for the public health. In this study, we utilized a high-fat diet-induced GDM model to evaluate the effects o...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiao, Ya, Chen, Weihao, Chen, Ruixue, Luo, Anling, Chen, Dayi, Liang, Qiuer, Liu, Tianhao, Chen, Xudong, Tan, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414337/
https://www.ncbi.nlm.nih.gov/pubmed/32802120
http://dx.doi.org/10.1155/2020/2953502
_version_ 1783568956071608320
author Xiao, Ya
Chen, Weihao
Chen, Ruixue
Luo, Anling
Chen, Dayi
Liang, Qiuer
Liu, Tianhao
Chen, Xudong
Tan, Wei
author_facet Xiao, Ya
Chen, Weihao
Chen, Ruixue
Luo, Anling
Chen, Dayi
Liang, Qiuer
Liu, Tianhao
Chen, Xudong
Tan, Wei
author_sort Xiao, Ya
collection PubMed
description OBJECTIVE: Gestational diabetes mellitus (GDM) is a pathological condition, affecting an increasing number of pregnant women worldwide. Safe and effective treatment for GDM is very important for the public health. In this study, we utilized a high-fat diet-induced GDM model to evaluate the effects of LBP on GDM and examined the changes of exosomal microRNA expression profiling to decipher the potential underlying mechanism of LBP. METHODS: Female C57BL/6J mice were fed a control diet, HFD, or 150 mg/kg LBP-supplemented HFD for 6 weeks before conception and throughout gestation. Oral glucose tolerance test and plasma lipid levels were determined, and liver histopathology was assessed. Sequencing was used to define the microRNA expression profiling of plasma exosomes in the three groups of mice, and protein expression levels of the candidate target genes were analyzed. RESULTS: LBP significantly relieved glucose intolerance, abnormal plasma lipid levels, and pathomorphological changes of liver histopathology in HFD-induced GDM mice. Moreover, we found that this effect of LBP was mediated by downregulation of the increase of 6 miRNAs (miR-93-3p, miR-188-5p, miR-466k, miR-1188-5p, miR-7001-3p, and miR-7115-5p) and reversing the increase of the protein expression of CPT1A, which is the target gene of miR-188-5p. CONCLUSIONS: Our findings provide novel insights into the biological activities of LBP in the treatment of GDM.
format Online
Article
Text
id pubmed-7414337
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-74143372020-08-14 Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice Xiao, Ya Chen, Weihao Chen, Ruixue Luo, Anling Chen, Dayi Liang, Qiuer Liu, Tianhao Chen, Xudong Tan, Wei Evid Based Complement Alternat Med Research Article OBJECTIVE: Gestational diabetes mellitus (GDM) is a pathological condition, affecting an increasing number of pregnant women worldwide. Safe and effective treatment for GDM is very important for the public health. In this study, we utilized a high-fat diet-induced GDM model to evaluate the effects of LBP on GDM and examined the changes of exosomal microRNA expression profiling to decipher the potential underlying mechanism of LBP. METHODS: Female C57BL/6J mice were fed a control diet, HFD, or 150 mg/kg LBP-supplemented HFD for 6 weeks before conception and throughout gestation. Oral glucose tolerance test and plasma lipid levels were determined, and liver histopathology was assessed. Sequencing was used to define the microRNA expression profiling of plasma exosomes in the three groups of mice, and protein expression levels of the candidate target genes were analyzed. RESULTS: LBP significantly relieved glucose intolerance, abnormal plasma lipid levels, and pathomorphological changes of liver histopathology in HFD-induced GDM mice. Moreover, we found that this effect of LBP was mediated by downregulation of the increase of 6 miRNAs (miR-93-3p, miR-188-5p, miR-466k, miR-1188-5p, miR-7001-3p, and miR-7115-5p) and reversing the increase of the protein expression of CPT1A, which is the target gene of miR-188-5p. CONCLUSIONS: Our findings provide novel insights into the biological activities of LBP in the treatment of GDM. Hindawi 2020-07-30 /pmc/articles/PMC7414337/ /pubmed/32802120 http://dx.doi.org/10.1155/2020/2953502 Text en Copyright © 2020 Ya Xiao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xiao, Ya
Chen, Weihao
Chen, Ruixue
Luo, Anling
Chen, Dayi
Liang, Qiuer
Liu, Tianhao
Chen, Xudong
Tan, Wei
Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice
title Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice
title_full Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice
title_fullStr Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice
title_full_unstemmed Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice
title_short Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice
title_sort exosomal microrna expression profiling analysis of the effects of lycium barbarum polysaccharide on gestational diabetes mellitus mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414337/
https://www.ncbi.nlm.nih.gov/pubmed/32802120
http://dx.doi.org/10.1155/2020/2953502
work_keys_str_mv AT xiaoya exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT chenweihao exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT chenruixue exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT luoanling exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT chendayi exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT liangqiuer exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT liutianhao exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT chenxudong exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice
AT tanwei exosomalmicrornaexpressionprofilinganalysisoftheeffectsoflyciumbarbarumpolysaccharideongestationaldiabetesmellitusmice